Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Detection of Novel Mutations in the FABP3 Promoter Region and Association Analysis with Intramuscular Fat Content in Pigs

돼지 FABP3 Promoter 부위 내 신규 돌연변이 탐색과 근내지방도와의 연관성 분석

  • Kim, J.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Park, E.W. (National Livestock Research Institute, R.D.A.) ;
  • Park, J.J. (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi, B.W. (National Livestock Research Institute, R.D.A.) ;
  • Kim, T.H. (National Livestock Research Institute, R.D.A.) ;
  • Seo, B.Y. (Division of Applied Life science, Gyeongsang National University) ;
  • Cheong, I.C. (National Livestock Research Institute, R.D.A.) ;
  • Lim, H.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Oh, S.J. (National Livestock Research Institute, R.D.A.) ;
  • Lee, J.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang Natioal University)
  • 김재환 (경상대학교 응용생명과학부) ;
  • 박응우 (농촌진흥청 축산연구소) ;
  • 박정진 (경상대학교 응용생명과학부) ;
  • 최봉환 (농촌진흥청 축산연구소) ;
  • 김태헌 (농촌진흥청 축산연구소) ;
  • 서보영 (경상대학교 응용생명과학부) ;
  • 정일정 (농촌진흥청 축산연구소) ;
  • 임현태 (경상대학교 응용생명과학부) ;
  • 오성종 (농촌진흥청 축산연구소) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부)
  • Published : 2005.02.28
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Abstract

Intramuscular fat content(lMF) is considered as one of major economic traits in the pig breeding and industry. In general, high IMF results in better meat quality. Several approaches to detect quantitative trait 10ci( QTL) for IMF indicated a strong possibility of the existence of a QTL related to IMF between the microsatellite marker SW71 and SW1881 on SSC6q. Porcine FABP3 has been considered as a candidate gene affecting IMF due to its physiological roles and position on the pig genome. Two novel mutations, g.-114T> C and g.-158T>G were detected by duplicate sequencing of the porcine FABP3 promoter region. These two mutations were identified as absolute linkage disequilibrium. The g.-158T> G mutation was used for investigating relationships with growth and fat deposition traits. The GG genotype of the g.-158T> G polymorphism showed highly negative effects(P< 0.01) on body weights at 3 and 12 weeks of age, and a positive effect(P< 0.05) on IMF. However, backfat thickness(BF) and carcass fat(CF) content were not significantly associated with the genotype. The result indicates that the novel mutations, identified in this study, could be utilized as possible genetic markers to improve IMF, independent with BF.

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References

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