Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Separation and Purification of Angiotensin Converting Enzyme Inhibitory Peptides derived from Goat's Milk Whey Hydrolysates

산양유 Whey로부터 ACE 억제 Peptide의 분리 및 정제

  • Lee, K.J. (Division of Animal Science and Technology, College of Agriculture and Life Sciences, Gyeongsang National University, Institute of Agriculture & Life Sciences) ;
  • Kim, S.B. (Dairy Science Division, National Livestock Research Institute, RDA) ;
  • Ryu, J.S. (Division of Animal Science and Technology, College of Agriculture and Life Sciences, Gyeongsang National University, Institute of Agriculture & Life Sciences) ;
  • Shin, H.S. (Nam Yang Research & Development Center) ;
  • Lim, J.W. (Division of Animal Science and Technology, College of Agriculture and Life Sciences, Gyeongsang National University, Institute of Agriculture & Life Sciences)
  • 이계준 (경상대학교 농업생명과학대학 동물자원과학부, 농업생명과학연구원) ;
  • 김상범 (농촌진흥청 축산연구소) ;
  • 류진수 (경상대학교 농업생명과학대학 동물자원과학부, 농업생명과학연구원) ;
  • 신현수 (남양유업 (주) 중앙연구소) ;
  • 임종우 (경상대학교 농업생명과학대학 동물자원과학부, 농업생명과학연구원)
  • Published : 2005.02.28
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Abstract

ACE-inhibitory peptides derived from goat's whey hydrolyzed by various proteolytic enzymes were separated and purified for antihypertension materials. The highest ACE-inhibitory activity of goat's whey hydrolysates was 85.5 % by pepsin for 72 hrs. Also the highest ACE-inhibitory activity of goat's whey hydrolysates was F-4 by pepsin for 72 hrs by Sephadex G-25 gel chromatograms. F-4e and F-4ed from F-4 by RP-HPLC to first and second purification were the highest in ACE-inhibitory activity, respectively. The most abundant amino acid was leucine(I 8.54 %) in F-4ed of ACE-inhibitory peptides after second purification. Amino acid sequence of F-4ed of ACE-inhibitory peptides showed Leu-Lys-Asp-Tyr-Gly-GlyVal- Ser-Leu and Leu-Gly-Asp-Gly-Ala-Gly- Asp-Val-Ala-Phe. $IC_{50}$ calibrated in peptic hydrolysates(72 hrs), F-4, F-4e and F-4ed from goat's whey hydrolysates by pepsin for 72 hrs were 33.93, 28.75, 11.74 and 1.09 mg/ml, respectively. From the results of this experiment, goat's whey hydrolysate by pepsin was shown to have ACE-inhibitory activity.

Keywords

References

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