Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effects of opioid and non-opioid antagonists, pH and enzymes on Corchorus olitorius antinociception in mice

  • Zakaria Zainul Amiruddin (School of Biotechnology and Life Sciences, Universiti Industri Selangor) ;
  • Neelendran M (School of Biotechnology and Life Sciences, Universiti Industri Selangor) ;
  • Pubalan S (School of Biotechnology and Life Sciences, Universiti Industri Selangor) ;
  • Sulaiman MR (Department of Biomedical Sciences, Faculty of Medicine and Health Science, Universiti Putra Malaysia) ;
  • Fatimah CA (School of Biotechnology and Life Sciences, Universiti Industri Selangor)
  • Published : 2006.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was carried out to determine the involvement of opioid and non-opioid receptor and the effect of pH and enzymes on the recently reported antinociceptive activity of aqueous extract of Corchorus olitorius (AECO) leaves using the abdominal constriction test. The extract was prepared by soaking the dried powdered leaves of Corchorus (C.) olitorius in distilled water overnight, and the supernatant obtained was considered as a stock solution with 100% concentration/ strength. The extract, administered subcutaneously in the concentrations/ strength of 10, 50 and 100%, was found to show a significant concentration-independent antinociception. The 50% concentration AECO were further used to study on the above mentioned parameters. The extract exhibited: significant (P < 0.05) decreased in activity when pre-treated (s.c.) against 10 mg/kg naloxonazine, bicuculine (10 mg/kg), phenoxybenzamine (10 mg/kg), 10 mg/kg pindolol, and 5 mg/kg mecamylamme, but not 10 mg/kg naltrindole, 10 mg/kg atropine, respectively; significant (P < 0.05) decreased in activity after pre-treatment against 10% a-amylase, but not 1 % protease or 10% lipase and; significant (P < 0.05) decreased in activity after exposure to alkaline condition (pH between 9 and 13) while maintaining the activity at acidic condition, respectively. The C. olitorius leaves antinociception, which involved, at least in part, activation of $\mu-opioid,\;\alpha-and\;\beta-adrenergic$, and nicotinic receptors, was found to decrease under alkaline condition and in the presence of $\alpha-amylase$.

Keywords

References

  1. Abu-Hadid AF, El-Shinawy MZ, El-Bethagy AS, Gaafer SA, Medany M. (1994) Studies on the production of off-season Jew's mallow (molokhia) in Egypt. Egypt J. Hartie. 21, 187-193.
  2. Baratti CM, Opezzo JW, Kopf SR. (1993) Facilitation of memory storage by the acetylcholine $M-2$ muscarinic receptor antagonist AF-DX 116. Behav. Neural Biol. 60, 69-74. https://doi.org/10.1016/0163-1047(93)90742-Z
  3. Barocelli E, Ballabeni V, Bertoni S, de Amici M, Impicciatore M. (2001) Evidence for specific analgesic activity of a muscarinic agonist selected among a new series of acetylenic derivatives. Life Sci. 68, 1775-1785. https://doi.org/10.1016/S0024-3205(01)00973-0
  4. Bentley GA, Newton SH, Starr J. (1981) Evidence for an action of morphine and the enkephalins on sensory nerve endings in the mouse peritoneum. Br. J. Pharmacol. 73, 325-332.
  5. Bentley GA, Newton SH, Starr J. (1983) Studies on the antinociceptive action of ${\alpha}$-agonist drugs and their interactions with opioid mechanisms. Br. J. Pharmacol. 79, 125-134. https://doi.org/10.1111/j.1476-5381.1983.tb10504.x
  6. Bentley GA, Starr J. (1986) The antinociceptive action of some beta-adrenoceptor agonists in mice. Br. J. Pharmacol. 88, 515-521. https://doi.org/10.1111/j.1476-5381.1986.tb10231.x
  7. Bowman WC, Rand MJ. (1980) Textbook of pharmacology. 2nd ed. London: Blackwell Scientific Publications.
  8. Calixto JB, Beirith A, Ferreira J, Santos AR, Cechinel Filho V, Yunes RA. (2000) Naturally occuring antinociceptive substances from plants. Phytother. Res. 14, 401-418. https://doi.org/10.1002/1099-1573(200009)14:6<401::AID-PTR762>3.0.CO;2-H
  9. Clojnacka-Wojcik E, Klodzinska A, Deren-Wesolek A. (1994) Involvement of $5-HT_{2C}$ receptors in the M-chlorophenylpiperazine-induced antinociception in mice. Pol. J. Pharmacol. 46, 423-428.
  10. Correa CR, Kyle DJ, Chakraverty S, Calixto JB. (1996) Analgesic profile of the pseudopeptide $B_2$ bradykinin receptor antagonist NPC 18688 in mice. Br. J. Pharmacol. 117, 552-558.
  11. Costa LG, Doctor SV, Murphy SD. (1982) Antinociceptive and hypothermic effects of trimethyltin. Life Sci. 31, 1093-1102. https://doi.org/10.1016/0024-3205(82)90082-0
  12. Dambisya YM, Lee TL, Sathivalu V, Mat Jais AM. (1999) Influence of temperature, pH and naloxone on the antinociceptive activity of Channa striatus(Haruan) extracts in mice. J. Ethnopharmacol. 66, 181-186. https://doi.org/10.1016/S0378-8741(98)00169-X
  13. Dambisya YM, Lee TL. (1995) Effects of L-NAME, L-NMMA and L-arginine on the analgesic effects of morphine in mice. Methods Find. Exp. Clin. Pharmacol. 17, 577-582.
  14. di Rosa M, Giroud JP, Willoughby DA. (1971) Studies of the mediators of the acute inflammatory response induced in rat in different site by carrageenan and turpentine. J. Path. 104, 15-29. https://doi.org/10.1002/path.1711040103
  15. Ginsburg V, Robbins P. (1981) Biology of carbohydrates. 2nd ed. New York: Wiley-Interscience.
  16. Gupta M, Mazumder UK, Pal D, Bhattacharya S, Chakrabarty S. (2003) Studies on brain biogenic amines in methanolic extract of Cuscuta reflexa Roxb. and Corchorus olitorius Linn. seed treated mice. Acta Pol. Pharm. 60, 207-210.
  17. Hendershot LC, Forsaith J. (1959) Antagonism of the frequency of phenylbenzoquinone induced writhing in the mouse by weak analgesics and non-analgesics. J. Pharmacol. Exp. Ther. 125, 237-240.
  18. Innami S, Ishida H, Nakamura K, Kondo M, Tabata K, Koguchi T, Shimizu J, Furusho T. (2005) Jew's mellow leaves (Corchorus olitorius) suppress elevation of postprandial blood glucose levels in rats and humans. Int. J. Vito Nutr. Res. 75, 39-46. https://doi.org/10.1024/0300-9831.75.1.39
  19. Kaneda N, Pezzuto JM, Soejarto DD, Kinghorn AD, Farnworth NR, Santisuk T, Tuchinda P, Udchachon J, Reutrakul V. (1991) Plant anticancer agents, XLVIII. New cytotoxic flavonoids from Muntingia calabura roots. J. Nat. Prod. 54, 196-206. https://doi.org/10.1021/np50073a019
  20. Katzung BG. (1995) Basic and clinical pharmacology (6th ed.). Appleton and Lange, Connecticut, U.S.A.
  21. Kirtikar KR, Basu BD. (1975) Indian medicinal plants (4th ed.). Jayyed Press, New Delhi, India.
  22. Mat Jais MA, Dambisya YM, Lee TL. (1997) Analgesic activity of Channa striatus (haruan) extracts in mice. J. Ethnopharmacol. 57, 125-130. https://doi.org/10.1016/S0378-8741(97)00057-3
  23. Negm S, EI-Shabrawy O, Arbid M, Radwan AS. (1980) Toxicological study of the different organs of Corchorus olitorius L. plant with special reference to their cardiac glycosides content. Z. Ernahrungswiss. 19, 28-32. https://doi.org/10.1007/BF02021069
  24. Ohtani K, Okai K, Yamashita U, Yuasa I, Misaki A. (1995) Characterization of an acidic polysaccharide isolated from the leaves of Corchorus olitorius(Moroheiya). Biosci. Biotechnol. Biochem. 59, 378-381. https://doi.org/10.1271/bbb.59.378
  25. Ono M, Satoh T. (1992) Pharmacological studies on Iappoconitine: Possible interaction with endogenous noradrenergic and serotonergic pathways to induce antinociception. Jpn. J. Pharmacol. 58, 251-257. https://doi.org/10.1254/jjp.58.251
  26. Peres MTLP, Delle Monache F, Pizollatti MG, Santos ARS, Beirith A, Calixto JB, Yunes RA. (1998) Analgesic compounds of Croton urucurana Baillon. Pharmacochemical criteria used in their isolation. Phytother. Res. 12, 209-214. https://doi.org/10.1002/(SICI)1099-1573(199805)12:3<209::AID-PTR215>3.0.CO;2-P
  27. Pieretti S, Dal Piaz V, Matucci R, Giovannoni MP, Galli A. (1999) Antinociceptive activity of a 3(2H)-pyridazinone derivative in mice. Life Sci. 65, 1381-1394. https://doi.org/10.1016/S0024-3205(99)00377-X
  28. Santos AR, Filho VC, Yunes RA, Calixto JB. (1995) Analysis of the mechanism underlying the antinociceptive effect of the extracts of plants from the genus Phyllanthus. Gen. Pharmacol. 26, 1499-1506.
  29. Sharaf A, Kamel SH, Salama A, Arbid MS. (1979) Oestrogenicity of Corchorus olitorius L. seed oil. Egyptian J. Vet. Med. 14, 87-93.
  30. Siegmund EA, Cadmus RA, Lu G. (1957) A method for evaluating both non-narcotic and narcotic analgesics. Proc. Soc. Exp. Biol. 95, 729-731.
  31. Spector WG. (1962) The inflammatory response. J. Path. Bacteriol. 84, 391-403. https://doi.org/10.1002/path.1700840213
  32. Su BN, Jung Park E, Vigo JS, Graham JG, Cabiess F, Fong HH, Pezzuto JM, Kingorn AD. (2003) Activity-guided isolation of the chemical constituents of Muntingia calabura using a quinone reductase induction assay. Phytochemistry 63, 335-341. https://doi.org/10.1016/S0031-9422(03)00112-2
  33. Sulaiman MR, Somchit MN, Israf DA, Ahmad Z, Moin S. (2004) Analgesic effect of Melastoma malabathricum ethanolic extract in mice. Fitoterapia 75, 667-672. https://doi.org/10.1016/j.fitote.2004.07.002
  34. Tripathi KD. (1994) Essentials of Medical Pharmacology (3rd ed.) Jaypee Brothers Medical Publishers Ltd., New Delhi, India.
  35. Verma A, Kulkarni SK. (1991) Alpha 2-adrenoceptor-and D2-dopamine receptor-mediated analgesic response of B-HT 920. J. Pharm. Pharmacol. 43, 131-133.
  36. Watt JM, Breyer-Brandwijk MG. (1962) The medicinal and poisonous plants of southern and eastern Africa. (2nd ed.). E&S Livingstone, London, Britain.
  37. Yen GC, Chen HY, Peng HH. (2001) Evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants. Food Chem. Toxicol. 39, 1045-1053. https://doi.org/10.1016/S0278-6915(01)00053-9
  38. Yoshikawa M, Shimada H, Saka M, Yoshizumi S, Yamahara J, Matsuda H. (1997) Medicinal foodstuffs. V. Moroheiya. (1): Absolute stereostructures of corchoionosides A, B, and C, histamine release inhibitors from the leaves of Vietnamese Corchorus olitorius L. (Tiliaceae). Chem. Pharm. Bull. 45, 464-469. https://doi.org/10.1248/cpb.45.464
  39. Zakaria ZA, Corazon CA, Asma H, Sulaiman MR, Arifah AK, Somchit MN, Mat Jais AM, Johari R, Kirisnaveni K, Punnitharanni D, Safaru M, Valsala R. (2005) The analgesic, anti-inflammatory and antipyretic activities of Corchorus olitorius aqueous extract in mice. Poster presented at the 20th Scientific Meeting of the Malaysian Society of Pharmacology and Physiology, 25th-27th April 2005b, Penang, Malaysia.
  40. Zakaria ZA, Mat Jais AM, Sulaiman MR, Somchit MN. (2004) Effects of a-amylase, protease and lipase on Haman (Channa striatus) mucus extracts antinociceptive activity in mice. Pakistan J. Biol. Sci. 7, 2202-2207. https://doi.org/10.3923/pjbs.2004.2202.2207
  41. Zakaria ZA, Safarul M, Valsala R, Sulaiman MR, Fatimah CA, Mat Jais AM. (2005) Influence of temperature on the opioid-mediated antinociceptive activity of Corchorus olitorius L. in mice. Naunyn-Schmiedeberg's Arch. Pharmacol. 372, 55-62. https://doi.org/10.1007/s00210-005-1089-8
  42. Zakaria ZA, Sulaiman MR, Somchit MN, Mat Jais AM. (2005) Effects of various antagonists on Haman (Channa striatus) fillet extract antinociception in mice. Can. J. Physiol. Pharmacol. 83, 635-642. https://doi.org/10.1139/y05-050
  43. Zeghichi S, Kallithkara S, Simopoulus AP. (2003) Nutritional composition of Molokhia (Chorchorus olitorius) and Stamnagathi (Cichorium spinosum). In Plants in Human Health and Nutrition Policy. Edited by A.P. Simopoulus and C. Gopalan. Karger, Basel, pp. 1-21.
  44. Zimmermann M. (1983). Ethical Guidelines for Investigations of Experimental Pain in Conscious Animals. Pain 16, 109-110. https://doi.org/10.1016/0304-3959(83)90201-4