Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparison of Antioxidant Activities of Enzymatic and Methanolic Extracts from Ecklonia cava Stem and Leave

감태(Ecklonia cava) 줄기 및 잎의 효소적 추출물과 메탄올 추출물에 의한 항산화 활성비교

  • Lee, Seung-Hong (Faculty of Applied Marine Biotechnology, Cheju National University) ;
  • Kim, Kil-Nam (Faculty of Applied Marine Biotechnology, Cheju National University) ;
  • Cha, Seon-Heui (Faculty of Applied Marine Biotechnology, Cheju National University) ;
  • Ahn, Gin-Nae (Faculty of Applied Marine Biotechnology, Cheju National University) ;
  • Jeon, You-Jin (Faculty of Applied Marine Biotechnology, Cheju National University)
  • 이승홍 (제주대학교 해양생물공학과) ;
  • 김길남 (제주대학교 해양생물공학과) ;
  • 차선희 (제주대학교 해양생물공학과) ;
  • 안긴내 (제주대학교 해양생물공학과) ;
  • 전유진 (제주대학교 해양생물공학과)
  • Published : 2006.09.01
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Abstract

In this study, antioxidant activities of enzymatic and methanolic extracts from E. cava stem and leave were evaluated by measuring the scavenging activities on 1,1 diphenyl 2 picrylhydrazyl (DPPH), hydroxyl radical, hydrogen peroxide and the inhibitory effects on DNA damage induced by oxidative stress of cells. Enzymatic extracts were prepared by enzymatic hydrolysis of both stem and leave using food grade five different carbohydrases (Viscozyme, Celluclast, AMG, Termamyl, Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase). The enzymatic extracts were lower than methanolic extracts in polyphenol contents, but higher in extraction yield by approximately 30%. The enzymatic extracts were superior to methanolic extracts in DPPH and H2O2 scavenging activities and DNA damage protective effect. There were no significant antioxidant activity difference between stem and leave, but the extracts of leave were relatively better than those of stem. In this study it is suggested that E. cava stem as well as its leave would be a good raw materials for antioxidants compound extraction and enzymatic hydrolysis would be a good strategy to prepare antioxidant extracts from seaweeds.

본 연구에서는 제주 연안에 서식하는 갈조류 중 감태의 줄기와 잎의 항산화활성을 비교하기 위하여 감태 줄기와 잎으로부터 효소적 가수분해와 메탄올을 이용하여 각각의 추출물을 제조한 후 이들로부터 활성산소종의 소거활성 및 세포의 산화적 손상 억제활성을 포함하는 항산화활성에 대한 차이를 비교 검토하였다. 여기에서 효소적 가수분해를 이용하여 제조된 감태의 효소적 추출물은 메탄올 추출물에 비하여 폴리페놀 함량은 낮았으나, 수율은 약 30% 이상 높게 나타났다. 감태는 줄기와 잎의 모든 부분에서 항산화활성이 우수하게 나타났으며, 두 부위 간의 유의적인 차이는 없었으나, 대체로 잎의 추출물이 줄기의 추출물보다 약간 우수한 항산화효과를 나타내었다. 이러한 결과는 감태의 잎 뿐만 아니라 줄기 또한 마찬가지로 우수한 생리활성 재료임을 확인할 수 있었다. 그리고 메탄올 추출물에 비해 효소적 추출물은 hydroxyl radical 소거활성을 제외한 전반적인 항산화효과 분석에 있어서 더 유용한 경향을 보였다. 이와 같은 결과는 여러 가지 효소들이 복합되어진 효소가 세포벽에 있는 섬유질이나 당단백질 혹은 알긴산 고분자물질 등을 분해시키는 작용을 하여 활성물질들이 원활히 추출될 수 있도록 유도해 주는 작용을 하였기 때문이라고 사료된다. 그리고 높은 수율을 바탕으로 한 수용성의 추출물이기 때문에 그들이 가지고 있는 생리활성물질을 식품산업에 쉽게 응용시킬 수 있으며, 유기용매와 같은 화학약품을 사용하였을 때에 발생할 수도 있는 안전성에 대한 문제도 해결할 수 있을 것이다. 이상의 결과로 감태는 잎 뿐만 아니라 줄기도 잠재적 의약품 소재 및 기능성식품 소재로서의 가능성이 충분하다고 판단된다.의 서로 반대되는 위치에서 온도차가 13.7도에서 -8.3도까지 차이가 나는 것으로 관찰되었으며, 미디어 높이 위치의 변화에 따라서도 21도에서 2도가지 차이를 나타냈다. 바이오필터 함수비는 실험기간 동안 지속적으로 변화가 발생하였는데, 스팀이 제공되는 동안에는 미디어 함수비가 훨씬 빠른 속도로 증가됨이 관찰되어 졌다.EX>$4.9{\sim}5.1^{\circ}Brix$ 수준이었으며, 소형과와 기형과는 S-3에서 많이 나왔다. 이상 연구결과에서 입도분포가 1.2-5mm인 것이 바람직한 것으로 나타났다.omopolysaccharides로 확인되었다. EPS 생성량이 가장 좋은 Leu. kimchii GJ2의 평균 분자량은 360,606 Da이었으며, 나머지 두 균주에 대해서는 생성 EPS 형태와 점도의 차이로 미루어 보아 생성 EPS의 분자구조와 분자량이 서로 다른 것으로 판단하였다.TEX>개로 통계학적으로 유의한 차이가 없었다. Heat shock protein-70 (HSP70)과 neuronal nitric oxide synthase (nNOS)에 대한 면역조직화학검사에서 실험군 Cs2군의 신경세포가 대조군 12군에 비해 HSP70과 nNOS의 과발현을 보였으며, 이는 통계학적으로 유의한 차이를 보였다(p<0.05). nNOS와 HSP70의 발현은 강한 연관성을 보였고(상관계수 0.91, p=0.000), nNOS를 발현하는 세포가 동시에 HSP70도 발현함을 확인할 수 있었다. 결론: 우리는 cyclosporin A가 토끼의 25분간의 척수허혈에 대해 척수보호 효과가 있었으며 이는 HSP70의 과발현과 연관이 있으리라 생각한다.

Keywords

References

  1. Wiseman H. 1996. Dietary influences on membarane function; important in protection against oxidative damage and disease. Nutr Biochem 7: 2-6 https://doi.org/10.1016/0955-2863(95)00152-2
  2. Gung GT, Ju IO, Choi JS, Hong JS. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT (Omija) seed. Korean J Food Sci Technol 32: 928-935
  3. Cook NC, Samman S. 1996. Flavonoids chemistry, mechanism, cardioprotective effects and dietary source. Nutr Biochem 7: 66-76 https://doi.org/10.1016/0955-2863(95)00168-9
  4. 강제원. 1968. 한국동식물도감(해조류). 삼화출판사, 서울. p 155-157
  5. Nagayama K, Iwamura Y, Shibata T, Hirayama I, Nakamura T. 2002. Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J Antimicrob Chemoth 50: 889-893 https://doi.org/10.1093/jac/dkf222
  6. Okai Y, Higashi-Okai K, Ishizaka S, Ohtani K, Matsui- Yuasa I, Yamashita U. 1998. Possible immunodulating activities in an extract of edible brown alga, Hijikia fusiforme (Hijiki). J Sci Food Agric 76: 56-62 https://doi.org/10.1002/(SICI)1097-0010(199801)76:1<56::AID-JSFA927>3.0.CO;2-L
  7. Chen CY, Chou HN. 2002. Screening of red algae filaments as a potential alternative source of eicosapentaenoic acid. Mar Biotechnol 4: 189-192 https://doi.org/10.1007/s10126-002-0002-4
  8. Frlich I, Riederer P. 1995. Free radical mechanisms in dementia of Alzheimer type and the potential for antioxidant treatment. Drug Res 45: 443-449
  9. Heo SJ, Jeon YJ. 2005. Antioxidant effect and protecting effect against cell damage by enzymatic hydrolysates from marine algae. Food Ind Nutr 10(1): 31-41
  10. Heo SJ, Jeon YJ, Lee J, Kim HT, Lee KW. 2003a. Antioxidant effect of enzymatic hydrolyzate from a kelp, Ecklonia cava. Algae 18: 341-347 https://doi.org/10.4490/ALGAE.2003.18.4.341
  11. Kim KN, Lee KW, Song CB, Jeon YJ. 2006. Cytotoxic activities of green and brown seaweeds collected from Jeju island against four tumor cell lines. J Food Sci Nutr 11: 17-24 https://doi.org/10.3746/jfn.2006.11.1.017
  12. Yasantha A, Jeon YJ. 2005. Screening for angiotensin-I converting enzyme inhibitory activity of Ecklonia cava. J Food Sci Nutr 10: 134-139 https://doi.org/10.3746/jfn.2005.10.2.134
  13. Cha SH, Ahn GN, Heo SJ, Kim KN, Lee KW, Song CB, Cho SM, Jeon YJ. 2006. Screening of extracts from marine green and brown algae in Jeju for potential marine angiotensin-I converting enzyme (ACE) inhibitory activity. J Korean Soc Food Sci Nutr 35: 307-314 https://doi.org/10.3746/jkfn.2006.35.3.307
  14. AOAC. 1990. Official Methods of Analysis. 16th ed. Association of official analytical chemists, Virginia, USA
  15. Heo SJ, Lee KW, Song CB, Jeon YJ. 2003b. Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 18: 71-81 https://doi.org/10.4490/ALGAE.2003.18.1.071
  16. Shetty K, Curtis OF, Levin RE, Witkowsky R, Ang V. 1995. Prevention of vitrification associated with the in vitro shoot culture of oregano (Origanum vulgare) by Pseudomonas spp. J Plant Physiol 147: 447-451 https://doi.org/10.1016/S0176-1617(11)82181-4
  17. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  18. Chung SK, Osawa T, Kawakishi S. 1997. Hydroxyl radical scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem 61: 118-123 https://doi.org/10.1271/bbb.61.118
  19. Muller HE. 1985. Detection of hydrogen peroxide produced by microorganism on ABTS-peroxidase medium. Zentrabl Bakteriol Mikrobio Hyg 259: 151-158 https://doi.org/10.1016/S0176-6724(85)80045-6
  20. Singh NP, Graham MM, Singh V, Khan A. 1995. Induction of DNA single-strand breaks in human lymphocytes by low doses of X-rays. Int Radiat Biol 68: 563-569 https://doi.org/10.1080/09553009514551551
  21. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. 2000. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctiorius L.). J Korean Soc Food Sci Nutr 29: 1127-1132
  22. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. 1988. Study on the inhibitory effect of tannins and fiavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical. Biochem Pharm 56: 213-222
  23. Heo SJ, Park EJ, Lee KW, Jeon YJ. 2005 Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresource Technol 96: 1613-1623 https://doi.org/10.1016/j.biortech.2004.07.013
  24. Siriwardhana N, Lee KW, Kim SH, Ha JW, Jeon YJ. 2003. Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci Tech Int 9: 339-346 https://doi.org/10.1177/1082013203039014
  25. Oki T, Masuda M, Furuta S, Nishibia Y, Terahara N, Suda I. 2002. Involvement of anthocyanins and other phenolic compounds in radical-scavenging activity of purple-fleshed sweet potato cultivars. J Food Chem Toxicol 67: 1752- 1756
  26. Kim JG, Kang YM, Eum GS, Ko YM, Kim TY. 2003. Antioxidative activity and antimicrobial activity of extracts from medicinal plants (Akebia quinate Decaisn, Scirusfluviatilis A. Gray, Gardenia jasminoides for. grandiflora Makino). J Agric Life Sci 37(4): 69-75
  27. Park EJ, Kang MH. 2002. Application of the alkaline comet assay for detecting oxidative DNA damage in human biomonitoring. Kor J Nutr 35: 213-222
  28. Kang MH. 2003. Green vegetable juice reduces in vitro and in vivo DNA damage and plasma lipid concentrations. Food Ind Nutr 8(1): 8-14

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