Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Antler Velvet Ethanol Extract on Common Serum Chemistry Panels and Histopathological Change in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin

녹용 에탄올 추출물이 2,3,7,8 Tetrachlorodibenzo-p-dioxin에 노출된 랫드의 일반 혈액 화학 지수 및 조직 병변에 미치는 효과

  • Choi, Kyung-Yun (Dept. of Life Science, College of Biomedical & Health Science, Konkuk University) ;
  • Hwang, Seock-Yeon (Dept. of Clinical Lab. Science, Joosung College) ;
  • Lee, Su-Chan (Dept. of Life Science, College of Biomedical & Health Science, Konkuk University) ;
  • Kim, Si-Kwan (Dept. of Life Science, College of Biomedical & Health Science, Konkuk University)
  • 최경운 (건국대학교 의료생명대학 생명과학부) ;
  • 황석연 (주성대학 임상병리과) ;
  • 이수찬 (건국대학교 의료생명대학 생명과학부) ;
  • 김시관 (건국대학교 의료생명대학 생명과학부)
  • Published : 2006.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the effect of ethanol extract of antler velvet (EAV) on common serum chemistry panels and histopathological change in rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Administration of TCDD ($50{\mu}g/kg$ body weight) induced significant decrease in platelet count (p<0.01), creatine phosphokinase (CPK, p<0.01), lactatate dehydrogenase (LDH, p<0.05) and glucose (p<0.05) levels and increase in hemoglobin (p<0.05), aspartate aminotransferase (AST, p<0.01), alanine amino transferase (ALT, p<0.05) and lipase activities (p<0.05), and blood urea nitrogen (BUN, p<0.05), triglyceride (p<0.01) and low density lipoptotein cholesterol (LDL-C, p<0.05) levels. However, pretreatment of EAV at daily dose of 20 mg/kg b.w. from 1 wk before TCDD exposure for 5 wks attenuated the abnormality of the overall serum chemistry panels but statistical difference between TE and TA groups was observed only in testicular weight (p<0.01), LDH activity (p<0.05), glucose (p<0.05) and lipase activity (p<0.01). In addition, TCDD induced significant histopathological changes including swelling, fatty metamorphosis, and vacuolar degeneration in liver; edema in proximal and distal convoluted tubules, and glomerulus in kidney; severe atrophy of red purple and appearance of significant number of macrophage in spleen; prominent atrophy and decrease in immune cells in thymus. On the other hand, administration of EAV attenuated histopathological damage induced by TCDD. These results further suggest that administration of EAV attenuates TCDD induced testicular, liver, pancreatic, hematopoietic and nephrotic toxicities in rats.

본 연구에서는 TCDD에 노출된 랫드에 있어 녹용의 에탄올 추출물(EAV)이 혈액상(complete blood count) 및 임상화학지수(common serum chemistry panels) 및 장기별 조직 병변에 미치는 효과를 구명하기 위하여 수행되었다. 본 연구 결과 TCDD에 노출된 랫드의 혈액상 및 혈액화학적 지수 중 혈소판 수 감소(p<0.01), AST(p<0.01) 및 ALT (p<0.05) 활성 증가, CPK (p<0.01) 및 LDH(p<0.05) 활성 감소, BUN 활성 증가(p<0.05), glucose 함량 감소(p<0.05), lipase 활성 증가(p<0.05), TG(p<0.01) 및 LDH-cholesterol(p<0.05) 함량 증가가 대조군에 비하여 유의하게 변화한 것으로 관찰되었다. 특히 혈소판 수 감소, serum lipase 활성 증가, abdominal lipoprotein lipase(LPL) 활성 저하 및 male에서 serum estrogen 함량 증가는 동물 종과 관계없이 매우 재현성이 있게 나타나는 독성 반응으로 본 실험에서도 대조군 대비 현저한 차이를 보였다. 본 실험에서 TCDD-노출 랫드의 체중 감소는 복부 지방 조직내 LPL 활성 저화와 serum에 존재하는 HSL의 활성 증가와 관련이 있을 것으로 사료된다. 또한 장기별 무게에 있어서도 TCDD는 고환의 무게를 현저하게 감소시켰다(p<0.01). 한편, 녹용의 에탄올 추출물(EAV)은 TCDD에 노출된 랫드에 있어 LDH의 활성 감소(p<0.05), glucose 함량 감소(p<0.05), lipase 활성 증가(p<0.05), 고환 무게 감소(p<0.01)에 대하여 유의하게 억제하는 것으로 관찰되었다. TA군의 흉선은 거의 퇴화되어 CO 흉선 무게의 3.8%에 불과하였다. TCDD에 노출된 랫드에 EAV를 전처리하면 TA군과 비교하여 비록 무게 감소는 유의하게 억제되나 CO군과 비교하면 현저하게 작은 값을 나타냈다(p<0.01). 이상의 결과로 보아 녹용은 랫드에 있어 다이옥신에 의하여 야기되는 일반 혈액화학지수 및 조직 병변에 미치는 효과를 개선시킬 수 있다고 판단한다.

Keywords

References

  1. Tsujibo H, Miyake Y, Maruyama K, Inamori Y. 1987. Hypotensive compounds isolated from an alcohol extract of the unossified horn of Cervis elaphus L var xanthopyugus Milne-Edwang (Rokujo). Isolation of lysophosphatidylcholine as a hypotensive principle and structure-activity study of related compounds. Chem Pharm Bull 35: 654-659 https://doi.org/10.1248/cpb.35.654
  2. Kim KW, Park SW. 1982. A study on the hematopoiesis action of deer horn extract. Korea Biochem J 15: 151-157
  3. Yong JI. 1976. Studies on deer horn; The effect of deer horn on the liver and other organs of cholesterol administered rabbits. J Korea Pharm Sci 6: 26-42
  4. Han SH. 1970. Influence of antler (deer horn) on the enterochromaffin cells in the gastrointestinal mucosa of rats exposed to starvation, heat, cold and electric shock. J Catholic Medical College 19: 157-165
  5. Kang CK, Kim SW. 1989. A study on the biochemical and nutritional inquiry of antler. Korea J Food Nutr 2: 65-71
  6. Zinkle JG, Vos JG, Moore JA, Gupta BN. 1973. Hematologic and clinical chemistry effect of 2,3,7,8-tetrachlorodibenzo- p-dioxin in laboratory animals. Environmental Health Perspectives 9: 111-118
  7. Gasiewicz TZ, Neal RA. 1979. 2,3,7,8-Tetrachlorodibenzo- p-dioxin tissue distribution excretion and effect on clinical chemical parameter in guinea pigs. Toxicol Applied Pharmacol 51: 329-339 https://doi.org/10.1016/0041-008X(79)90475-7
  8. Brewster DW, Matsumura F. 1989. Differential effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin on adipose tissue lipoprotein lipase activity in the guinea pig, rat, hamster, rabbit and mink. Comp Biochem Physiol 93C: 49-53
  9. Enan E, Lui PCC, Matsumura F. 1992. 2,3,7,8-TCDD causes reduction in glucose uptake through glucose transports on the plasma membrane of the guinea pig adipocyte. J Environ Sci Health B27: 495-510
  10. Buu-Hoi NPH, Moore JA, Vos JG. 1972. Organism as targets of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in toxication. Naturxiss 59: 174-179
  11. Peterson RE, Theobald HM, Kimmel GL. 1993. Developmental and reproductive toxicity of dioxins and related compounds; cross-species comparisons. Crit Rev Toxicol 23: 283-335 https://doi.org/10.3109/10408449309105013
  12. Choi KY, Hwang SY, Kim SK. 2005. Ethanol extract of antler velvet attenuates testicular toxicity induced by 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) in rats. Korea J Food Sci Nutr 34: 1169-1174 https://doi.org/10.3746/jkfn.2005.34.8.1169
  13. Kim W, Hwang S, Lee H, Song H, Kim S. 1999. Panax ginseng protects the testis against 2,3,7,8-tetrachlorodibenzo-p-dioxin induced testicular damage in guinea pigs. British J Urol Int'l 83: 842-849
  14. Poland A, Knuston JC. 1982. 2,3,7,8-Tetrachlorodibenzo-p- dioxin and related halogenated aromatic hydrocarbons; examination of the mechanism of toxicity. Ann Rev Pharmacol Toxicol 22: 517-554 https://doi.org/10.1146/annurev.pa.22.040182.002505
  15. Safe S, Astroff B, Harris B. 1996. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related compounds as antiestrogens; characterization and mechanism of action. Pharmacol Toxicol 69: 400-409 https://doi.org/10.1111/j.1600-0773.1991.tb01321.x
  16. Ley CJ, Lees B, Stevenson JC. 1992. Sex- and menopause-associated changes in body-fat distribution. Am J Clin Nutr 55: 950-954 https://doi.org/10.1093/ajcn/55.5.950
  17. Wassom JS, Huff JE, Loprieno N. 1977/1978. A review of the genetic toxicology of chlorinated dibenzo-p-dioxins. Mutation Res 47: 141-160
  18. Jones G, Butler WH. 1974. A morphological study of the liver lesion induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats. J Pathol 112: 93-97 https://doi.org/10.1002/path.1711120204
  19. Yoo YS. 1999. Endocrine Disruptor. Korea Society of Environmental Administration 5: 331-337
  20. Kim YC. 2000. Developmental and reproductive toxicity of dioxins and related compounds. J NERI 5: 373-397
  21. McConnel EE. 1980. Acute and chronic toxity, carcinogenesis, reproduction, teratogenesis and mutagenesis in animals. Eisevier/North-Holland Biomedical Press, New York. p 241-266

Cited by

  1. Ethanol Extract ofAllium sativumAttenuates Testicular and Liver Toxicity Induced by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin in Rats vol.12, pp.1, 2009, https://doi.org/10.1089/jmf.2007.0620