Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Anti-apoptotic and neuroprotective effects of acupuncture techniques of tonification or sedation at LR3 on focal brain ischemic injury induced by intraluminal filament insertion in rats

태위(太衝)(LR3)에 대한 영수(迎隨)및 염전보사(捻轉補瀉)가 intraluminal filament 삽입술(揷入術)에 의하여 유발(誘發)된 백서(白鼠)의 focal ischemia에 미치는 영향(影響)

  • Park, Jong-Seung (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Shin University) ;
  • Na, Chang-Su (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Shin University) ;
  • Cho, Myeng-Rae (Dept. of Meridian and Acupoint, College of Oriental Medicine, Dong-Shin University) ;
  • Jung, Yeon-Jin (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
  • Jeong, Ji-Yeon (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
  • Kim, Won-Jae (Dental Science Research Institute, School of Dentistry Chonnam National University) ;
  • Choi, Chan-Hun (Dept. of Oriental Medicine College of Oriental Medicine, Dong-Shin University) ;
  • Youn, Dae-Hwan (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dong-Shin University)
  • 박종승 (동신대학교 한의과대학 경혈학교실) ;
  • 나창수 (동신대학교 한의과대학 경혈학교실) ;
  • 조명래 (동신대학교 한의과대학 침구학교실) ;
  • 정연진 (전남대학교 치의학전문대학원 치의학연구소) ;
  • 정지연 (전남대학교 치의학전문대학원 치의학연구소) ;
  • 김원재 (전남대학교 치의학전문대학원 치의학연구소) ;
  • 최찬헌 (동신대학교 한의과대학 생리학교실) ;
  • 윤대환 (동신대학교 한의과대학 경혈학교실)
  • Published : 2006.09.27
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Abstract

Objectives : Acupuncture using a tonification or sedation techniques method is used as a controlling the medication for an early stroke in the Korean medicine. LR3 has indicatons of headache, vertigo, facial paralysis, apoplexy, epiepsy as the source acupoint of a liver meridian. So this study is aims to investigate the anti-apoptotic and neuroprotective effects of acupuncture on the focal ischemia induced by intraluminal filament insertion in rats. Methods : The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The animals were divided into seven groups (n=8 in each group) : Normal, intactness group; Conrol group, no therapy group after being ischemia induced; MA-l, acupuncture perpendicularly without Tonification or Sedation techniques at LR3 after being ischemia induced; MA-2, acupuncture obliquely towards the knee at LR3 after being ischemia induced; MA-3, acupuncture obliquely towards the toe at LR3 after being ischemia induced; MA-4, acupuncture obliquely towards the knee and rotate 9 times in a clockwise direction at LR3 after being ischemia induced; MA-5, acupuncture obliquely towards the toe and rotate 6 times in a counterclockwise direction at LR3 after being ischemia induced. The anti-apoptotic and neuroprotective effects of Acupuncture techniques of tonification or sedation at LR3 are observed by mGluR5, Bax, Cresyl violet, ChAT-stain and NGF. Results : The intensity of mGluR5 and the density of ChAT was increased in MA-1 group. The intensity of Bax was decreased in MA-3, MA-4 group. The density of neurons stained by Cresyl violet and ChAT was increased in MA-2, MA-3, MA-4, MA-5 group. The density of neurons stained by NGF was only increased in MA-4 group. Conclusions : Our study suggests that acupuncture perpendicularly without Tonification or Sedation techniques and obliquely towards the knee and rotate 9 times in a clockwise direction(Tonifying technique) at LR3 after being ischemia induced at LR3 shows anti-apoptotic and neuroprotective effects on cholinergic neuron in focal cerebral ischemia of the stroke in rats.

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