Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antiproliferative effect of Houttuynia cordata Thunb was Associated with the Inhibiton of Cyclooxygenase-2 Expression in Human Breast Carcinoma Cells

인체유방암세포에서 Cyclooxygenase-2 활성 및 Prostaglandin E2 생성에 미치는 어성초 추출물의 영향

  • Jung, Il-Hong (Department of Internal Medical, Dongeui University College of Oriental Medicine) ;
  • Jo, In-Joo (Department of Internal Medical, Dongeui University College of Oriental Medicine) ;
  • Park, Cheol (Department of Biochemistry, Dongeui University College of Oriental Medicine) ;
  • Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine) ;
  • Park, Dong-Il (Department of Internal Medical, Dongeui University College of Oriental Medicine)
  • 정일홍 (동의대학교 한의과대학 폐계내과학교실) ;
  • 조인주 (동의대학교 한의과대학 폐계내과학교실) ;
  • 박철 (동의대학교 한의과대학 생화학교실) ;
  • 최영현 (동의대학교 한의과대학 생화학교실) ;
  • 박동일 (동의대학교 한의과대학 폐계내과학교실)
  • Published : 2006.06.25
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Abstract

Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is a traditional medicinal plant generally used in Oriental medicine therapy. In the present study, we investigated the effect of water extract of H. cordafa (WEHC) on the growth of human breast carcinoma MCF-7 cells. Exposure of MCF-7 cells to WEHC resulted in growth inhibition and apoptosis in a dose-dependent manner as measured by MTT assay and fluorescent microscope. The anti-proliferative effect of WEHC was associated with a dose-dependent up-regulation of cyclin-dependent kinase inhibitor p21 in a p53-independent fashion. We found WEHC decreased the levels of cyclooxygenase (COX)-2 and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1 , which was correlated with a decrease in prostaglandin $E_2{\;}(PGE_2)$ synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.

Keywords

References

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