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Effects of cultivated wild ginseng pharmacopuncture on lowering lipid and oxidative capacity in biochemical and molecular biological study in obese rats

산양산삼약침의 지질강하 및 항산화효과에 대한 생화학적 및 분자생물학적 검토

  • Choi, Eun-Ju (Department of Meridian & Acupoint, Oriental Medical College, Sangji University) ;
  • Lee, Joon-Moo (Department of Meridian & Acupoint, Oriental Medical College, Sangji University) ;
  • Won, Seung-Hwan (Department of Acupuncture & Moxibustion, Oriental Medical College, Sangji University) ;
  • Kwon, Ki-Rok (Department of Acupuncture & Moxibustion, Oriental Medical College, Sangji University)
  • 최은주 (상지대학교 한의과대학 경혈학 교실) ;
  • 이준무 (상지대학교 한의과대학 경혈학 교실) ;
  • 원승환 (상지대학교 한의과대학 침구학교실) ;
  • 권기록 (상지대학교 한의과대학 침구학교실)
  • Published : 2006.02.28

Abstract

Objectives : This study was carried out to identified the effects of distilled cultivated wild ginseng pharmacopuncture to the obesity. Methods : Cultivated wild ginseng pharmacopuncture was administered on the points of chung-wan(CV12), $Ch'{\breve{o}}nch'u$(ST25), and Chok-samni(ST36) on lowering lipid and oxidative capacity in biochemical and molecular biological aspects were investigated in obese rats fed with high fat diet. Results : 1. The contents of plasma ${\beta}-lipoprotein$ showed a tendency to decrease in the pharmacopuncture groups compared to the control group. In the pharmacopuncture groups, the values of ST25 and ST36 pharmacopuncture groups showed lower value. 2. The contents of plasma free fatty acids showed a tendency to decrease in pharmacopuncture groups compared to the control group. However, in the pharmacopuncture groups, the values were not significantly different. 3. Plasma triglyceride and glucose showed lower value in the ST25 pharmacopuncture groups compared with the other groups. 4. The activity of AST showed a tendency to decrease in the pharmacopuncture groups. However, the activity of ALT was not significantly different in all the treatment groups. 5. Plasma total cholesterol and LDL-cholesterol showed lower value in the ST25 and ST36 pharmacopuncture groups and HDL-cholesterol showed higher value in the CV12 pharmacopuncture groups than that of the other treatment groups. 6. Liver total cholesterol values didn't show significant difference in all the treatment groups, and triglyceride showed lower value in the pharmacopuncture groups. 7. The contents of plasma TEARS showed lower value in the ST25 pharmacopuncture group and contents of liver TBARS showed a tendency to decrease in the pharmacopuncture groups. However these values didn't show significant difference in the pharmaco puncture groups. 8. Liver super oxide dismutase activity showed higher value in the ST25 and ST36 pharmacopuncture groups, and the value of liver glutathione peroxidase and catalase activity showed a tendency to increase in the pharmacopuncture groups. However, these values showed no significant difference in the pharmacopuncture groups. 9. Expression of apo-B and E mRNA in liver cells was lower in the ST25 pharmacopuncture group than that of the other treatment groups. However, expression of $TNF-{\alpha}$ and leptin mRNA in adipose cell showed no difference among all the treatment groups. 10. ST25 pharmacopuncture group showed a good histological character of liver. It showed similar to that of normal group. However other treatment groups and control group showed slight vasodilation and slight fat accumulation. Conclusion : These results indicate that distilled cultivated wild ginseng pharmacopuncture suppressed adipose tissue mass and lipid peroxidation, and increased antioxidant capacity.

References

  1. Nielsen S, Jensen MD. Obesity and cardivascular disease is body structure a factor. Curr. Opin. Lipidol 1997;8(4):200–4.
  2. Lee KU, Lee HK, Koh CS, Min HK. Artificial induction of intravascular lipolysis by lipid-heparin infusion leads to insulin resistance in man. Diabetologia 1988;31(5):285–90.
  3. Kissebah AH, Alfarsi S, Adams PW, Wynn V. Role of insulin resistance in adipose tissue and liver in the pathogenesis of endogenous hypertriglyceridaemia in man. Diabetologia 1976;12(6):563–71. https://doi.org/10.1007/BF01220632
  4. Bray GA. Obesity increase risk for diabetes. Int. J. Obes. Relat. Metab. Disord 16(Suppl). 1992; 4:S13–S17.
  5. Graham S, Marshall J, Haughey B, Mittelman A, Swanson M, Zielezeny M, Byers T, Wilkinson G, West D. Dietary epidemiology of cancer of the colon in western New York. Am. J. Epidemiol 1988;128(3):490–503.
  6. Jain M, Cook GM, Davis FG, Grace MG, Howe GR, Miller AB. A case-control study of diet and colo-rectal cancer. Int. J. Cancer 1980;26(6):757–68. https://doi.org/10.1002/ijc.2910260609
  7. Van der HB, Bar PR, Kapplle LJ, de Wildt DJ. Dietary vitamin E levels affect outcome of permanent focal cerebral ischemia in rat. Stroke 1998;29(5):1002–6.
  8. Yang SQ, Lin HZ, Lane MD, Clemens M, Diehi AM. Obesity increase sensitivity to endotoin liver injury: implications for the pathogenesis of steatohepatitis. Proc. Natl. Acad. Sci. USA 1997;94(6):2557–62.
  9. Ronald M, Krauss, Mary W. Obesity : Impact on cardiovascular disease. Circulation 1998;98:1472–6.
  10. Robert HE, Ronald M, Krauss. American heart association call to action : Obesity as a major risk factor for coronary heart disease. Circulation 1998;97:2099–100.
  11. William HD. Childhood weight after adult morbidity and mortality. J. Nutr 1998;128:411S–414S.
  12. 허 준. 東醫寶鑑. 서울: 남산당. 1987 ; 73
  13. 申署謹. 600例 單純性肥滿患者與中醫分型的關係. 第2次全國中西醫結合 肥滿病硏究學術論文的要偏 1989:10.
  14. 劉抗華. 耳穴坤壓治療肥滿症 567例臨床觀察, 上海鍼灸雜誌 1986:4.
  15. 王 聲芳. 耳壓治療肥滿症 50例 小結. 四川中醫 1987:4, 5.
  16. 張忠志. 耳針對靑年男女體型的影響. 中國鍼灸 1989:5, 10.
  17. 趙銀龍. 鍼灸對單純性肥滿患者糖代謝的影響. 第2次全國中西醫結合肥滿病硏究學術論文的要編 1989:7.
  18. 李凝凜. 針刺減肥300例 療效分析. 上海鍼灸雜誌 1988:3, 7.
  19. 原桃仁. 肥滿的中醫治療. 第2次中國中西醫結合肥滿病硏究學術論文要的編 1989:7.
  20. 노성규, 강성길. 방풍수침이 고혈압 및 고지혈증에 미치는 영향. 경희 한의대 논문집 1985:8, 157–70.
  21. 이호섭, 변재영, 유윤조. 약침이 자발성 고혈압 백서의 혈압에 미치는 영향. 대한침구학회지 1996;13(1):422–428.
  22. 박원필, 권기록, 이은. 산양산삼약침이 고지방식이에 의한 당뇨병 유발 흰쥐에 미치는 영향. 대한약침학회지 2005;8(2):97–108. https://doi.org/10.3831/KPI.2005.8.2.097
  23. 대한약침학회. 약침요법 시술 지침서. 서울: 대한약침학회. 1999;13-4,112-8,138-203.
  24. 침구.경혈학교실 편저. 침구학. 서울: 집문당. 1988;190-1,196-7;371,382,730.
  25. 안준철, 임종국. 肝兪 및 中脘穴 艾灸가 백서의 혈청지질 변화에 미치는 영향. 대한침구학회지 1994;11(1):283–95.
  26. 이종현, 성낙기. 天樞穴에 대한 문헌적 고찰. 대전대학교 한의학 연구소. 한의학논문집 1995;4(1):249–56.
  27. 박원환. 內關 足三里穴의 하수오약침이 고콜레스테롤 혈증 병태 백서에 미치는 영향. 대한동의병리학회지 2000;14(1):135–47.
  28. 이건목, 김경식, 장종덕, 김기영. 附子 경구투여 및 足三里 약침이 정상 백서의 체중.혈청변화에 관한 연구. 대한침구학회지 1999;16(1):269–82.
  29. 林鍾國. 艾久生體反應의 文獻的考察. 東洋醫學 1976;13:63–8.
  30. Buge, J.A, Austa S.D, Microsomal lipid peroxidation. In Methods in Enzymology. Fleicher S., Packer L.(eds) Academic Press, London. 1978; 52: 302-9.
  31. Levander O.A., Deloach D.P., Morris V.C, Moser P.B. Platelet glutathione peroxidase activity as an index of selenium status in rats. J. Nutr. 1983;55–63,113.
  32. Flohe L, Becker R, Brigelius R, Lengfelder E, Otting F. Convenient as says for superoxide dismutase. CRC Handbook of free radicals and antioxidants in Biomedicine 1992:287–93.
  33. Johnson LH, Hakan Borg LA. A spectrophtometric method for determination of catalase activity in small tissue samples. Analytical Biochemistry 1988:174, 331–6. https://doi.org/10.1016/0003-2697(88)90554-4
  34. 崔述貴. 耳針治療肥滿550例 療效分析. 中醫藥新式 1986:5.
  35. Dahlqvist A., Johnsen J. Awarning against ear acupuncture used in the treatment of obesity. Lakartidningen 1987;(9):16, 38, 84.
  36. MV. Bogdanovich VL. Role of reflexotherapy in the complex treatment of neuroendocrine obesity. TerArkh 1985:10, 57, 97–100.
  37. 原桃仁. 肥滿的中醫治療. 第2次中國中西醫結合肥滿病硏究學術論文要的編 1989:7.
  38. 王秀風. 中風治療單純性肥滿44例 療效觀察. 中醫雜誌 1988:1.
  39. 趙銀龍. 鍼灸對單純性肥滿患者糖代謝的影響. 第2次全國中西醫結合肥滿病硏究學術論文的要編 1989:7.
  40. 孫廣培. 耳穴點壓加防風通聖丸治療肥滿症 147例. 四川中醫 1988:2, 6.
  41. 宗文九. 痰飮淺說. 上海: 上海科技. 1985;51-2.
  42. 朴元煥. 혈전증과 打撲性 充血 및 高脂血症에 順氣導痰湯 및 化瘀湯이 미치는 영향. 동국대학교 박사학위논문.1992.
  43. 崔承勳. 血府遂瘀湯이 혈전증과 피하혈종에 미치는 영향. 대한동의병리학회지 1987:2, 26–47.
  44. 崔承勳. 黃帝內經에서의 어혈의 인식에 대한 이론적 연구. 대한동의병리학회지 1988:3, 12–7.
  45. 呂明壓. 耳穴按壓法感肥 1000例 臨床療效報道. 貴州醫藥 1986;10(5).
  46. 殷榮亮. 耳穴點壓治療肥滿症 200例 療觀察. 協書中醫 1986:5, 7.
  47. 李士杰. 耳穴坤鍼感肥 253例 臨床觀察. 中國鍼灸 1986:3, 6.
  48. 吳武. 按摩治療肥感證 21例 效果觀察. 按摩與圖 1988:1.
  49. Ishikawa T, Suzukawa M. Effect of tea flavonoid supplementation on the susceptibility of low-density lipoprotein to oxidative modification. Am J Clin Nutr. 1997;66(2):261–6.
  50. 김종호. 임상생화학. 서울: 고려의학. 1998.
  51. Anderson JW. The nutritonal therapy of non-insulin dependent (typeII) diabetes. Diabetes Educator 1983:9, 13–8. https://doi.org/10.1177/014572178300900303
  52. Ueda H, Tanoue K. Growth-depressing and cholesterol- lowering effects of quillaja and tea saponins in chicks as influenced by diet composition. Anim. Sci. J 2000;71(4):393–9.
  53. 조수열, 박미리, 박원학, 정재홍. 콜레스테롤식이로 사육한 흰쥐의 혈장 및 간지질성분에 미치는 카페인 및 비타민 E의 영향. 한국영양식량학회지 1989;18(1):53–61.
  54. Barker J, Liu JP, Robertson EJ, Estratiadis A. Role of insulin-like growth factors in embryonic and postnatal growth. Cell 1993;75(1):73–82. https://doi.org/10.1016/S0092-8674(05)80085-6
  55. Hill JO, Lin D, Yakybu F, Peters JC. Development of dietary dbesity in rats : influence of amount and composition of dietary fat. Int. J. Obes. Relat. Metab. Disord 1992;16(5):321–33.
  56. Weigle DS. Appetite and the regulation of body composition. FASEB. J. 1994;8(3):302–310.
  57. 이준무, 이은. 삼황사심탕약침의 지질강하 및 항산화효과. 대한약침학회 2005;8(1):51–58. https://doi.org/10.3831/KPI.2005.8.1.051
  58. 이은, 최무영. 솔잎분말이 고콜레스테롤 급여 흰쥐의 체지질구성과 TBARS량에 미치는 영향. 한국식품과학회지 2000;32(5):1186–90.
  59. Lee, E. Effects of Green tea on serum lipid composition and TBARS in chicks with oxidized lipid. Sangji Univ. Life Sci. Research Ins 1998:51–6.

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