당귀약침(當歸藥鍼)이 소음 Stress를 받은 새끼 쥐의 신경세포 생성에 미치는 영향

Effect of Postnatal Angelicae Gigantis Radix Herb-acupuncture on Cell Proliferation in Offspring Rats with Prenatal Noise Stress during Pregnancy

Methods : 40 of Female rats were allowed to mate with 40 of male rats. Then, female rats were delivered of offspring rats, After birth 28 days, offspring rats were divided 8 groups, The normal group(Group A), the 10 mg/kg Angelicae gig antis radix~treated group(Group B), the 50 mg/kg Angelicae gigantis radix-treated group(Group C), the 100 mg/kg Angelicae gig antis radix-treated group(Group D), The control (noise-treated) group(Group E). the noise -10 mg/kg Angelicae gigantis radix-treated group(Group F), the noise-50 mg/kg Angelicae gigantis radix~treated group(Group G), and the noise-100 mg/kg Angelicae gigantis radix-treated group(Group H)(n = 5 in each group), From the 15th day of pregnancy, all rats were subcutaneously injected with 50 mg/kg BrdU once a day 30 min before the starting of experimental treatment. Rats of the prenatal noise-treated group were applied with 95 decibel supersonic machine sound for 1h once a day until delivery, After birth 28 days, offspring rats intraperitoneally injected with 50 mg/kg of BrdU and offspring rats were treated Angelicae gigantis radix Herb-acupunture on chungwan(CV12) for 7 consecutive days. For the detection of BrdU-positive cells and Ki-67 positive cells in hippocampus, immunohistochemistry was performed. Results : 1. The number of BrdU-positive cells in the dentate gyrus of noise-treated group was significantly decreased to normal group, and the Group F, G, H were significantly increased to control group. 2. The number of Ki-67 positive cells in the dentate gyrus of noise-treated group was significantly decreased to control group, and the Group G, H were significantly increased to control group. Conclusion : We concluded that postnatal Angelicae gigantis radix administration has effect on cell proliferation in offspring rats with prenatal noise stress during pregnancy.