Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Influence of Ginsenoside Rb1 on Brain Neurosteroid during Acute Immobilization Stress

  • Lee, Sang-Hee (Bioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology,Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Jung, Byung-Hwa (Bioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology) ;
  • Choi, Sang-Yoon (Korea Food Research Institute) ;
  • Kim, Sun-Yeou (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • H.Lee, Eun-Joo (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Chung, Bong-Chul (Bioanalysis & Biotransformation Research Center, Korea Institute of Science & Technology)
  • Published : 2006.07.01
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Abstract

This study examined whether or not acute stress is linked to increases in the neurosteroid levels, which is a well-known neurotransmitters associated with stress stimuli. The ginsenoside, Rb1, was tested in order to better understand its potential effects on altering the neurosteroid levels and ultimately attenuating stress. The optimal stressed condition was checked by measuring the 5a-dihydroprogesterone (DHP) and allopregnanolone (THP) levels in the brain after immobilization stress at various times. Based on this result, an acute stress model was set up to give 30 min of immobilization stress. The DHP and THP brain levels of the stressed mice were then investigated after administering Rb1 orally (10 mg/kg). These results were compared with the neurosteroid level in the stressed mice not given Rb1. Saline was administered orally to the nonstressed mice to check the placebo effect. Acute immobilization stress induced an increase in the THP and DHP concentration in the frontal cortex and cerebellum. When Rb1 was administered orally prior to immobilization stress, the THP level in the frontal cortex and cerebellum was significantly lower than that in the stressed animals not given Rb1. On the other hand, the DHP level was lower in the cerebellum only. This suggests that the metabolism of the brain neurosteroids is linked to psychological stress, and Rb1 attenuates the stressinduced increase in neurosteroids.

Keywords

References

  1. Barbaccia, M. L., Concas, A., Serra, M., and Biggio, G., Stress and neurosteroids in adult and aged rats. Exp. Gerontol., 33, 697-712 (1998) https://doi.org/10.1016/S0531-5565(98)00042-4
  2. Bhattacharya, S. K. and Murugandam, A. V., Adaptogenic activity of Withania somnifera: an experimental study using a rat model of chronic stress. Pharmacol. Biochem. Behav., 75, 547-555 (2003) https://doi.org/10.1016/S0091-3057(03)00110-2
  3. Chen, X. C., Zhu, Y. G., Zhu, L. A., Huang, C., Chen, Y., Chen, L. M., Fang, F., Zhou, Y. C., and Zhao, C. H., Ginsenoside Rg1 attenuates dopamine-induced apoptosis in PC12 cells by suppressing oxidative stress. Eur. J. Pharmacol., 473, 1-7 (2003) https://doi.org/10.1016/S0014-2999(03)01945-9
  4. Concas, A., Porcu, P., Sogliano, C., Serra, M., Purdy, R. H., and Biggio, G., Caffeine-induced increases in the brain and plasma concentrations of neuroactive steroids in the rat. Pharmacol. Biochem. Behav., 66, 39-45 (2000) https://doi.org/10.1016/S0091-3057(00)00237-9
  5. Lee, S. H., Jung, B. H., Kim, S. Y., and Chung, B. C., Determination of phytoestrogens using gas chromatography-mass spectrometric (GC-MS) in traditional medicine herbs. J. Nutr. Biochem., 15, 452-460 (2004) https://doi.org/10.1016/j.jnutbio.2004.01.007
  6. Lim, J. H., Wen, T. C., Matsuda, S., Tanaka, J., Maeda, N., Peng, H., Aburaya, J., Ishihara, K., and Sakanaka, M., Protection of ischemic hippocampal neurons by ginsenoside Rb1, a main ingredient of ginseng root. Neurosci. Res., 28, 191-200 (1997) https://doi.org/10.1016/S0168-0102(97)00041-2
  7. Lian, X. Y., Zhang, Z. Z., and Stringer, J. L., Anticonvulsant activity of ginseng on seizures induced by chemical convulsants. Epilepsia, 46, 15-22 (2005) https://doi.org/10.1111/j.0013-9580.2005.40904.x
  8. Lian, X. Y., Zhang, Z. Z., and Stringer, J. L., Protective effects of ginseng components in a rodent model of neurodegeneration. Ann. Neurol., 57, 642-648 (2005) https://doi.org/10.1002/ana.20450
  9. Mehendale, S., Aung, H., Wang, A., Yin, J. ., Wang, C. Z., Xie, J. T., and Yuan, C. S., American ginseng berry extract and ginsenoside Re attenuate cisplatin-induced kaolin intake in rats. Cancer. Chemother. Pharmacol., 56, 63-69 (2005) https://doi.org/10.1007/s00280-004-0956-1
  10. Mele, P., Oberto, A., Serra, M., Pisu, M. G., Floris, I., Biggio, G., and Eva, C., Increased expression of the gene for the Y1 receptor of neuropeptide Y in the amygdala and paraventricular nucleus of Y1R/LacZ transgenic mice in response to restraint stress. J. Neurochem., 89, 1471-1478 (2004) https://doi.org/10.1111/j.1471-4159.2004.02444.x
  11. O'Dell, L. E., Alomary, A. A., Vallee, M., Koob, G. F., Fitzgerald, R. L., Purdy, R. H., Ethanol-induced increases in neuroactive steroids in the rat brain and plasma are absent in adrenalectomized and gonadectomized rats. Eur. J. Pharmacol., 484, 241-247 (2004) https://doi.org/10.1016/j.ejphar.2003.11.031
  12. Ordyan, N. E. and Pivina, S. G., Anxiety levels and neurosteroid synthesis in the brains of prenatally stressed male rats. Neurosci. Behav. Physiol., 33, 899-903 (2003) https://doi.org/10.1023/A:1025952906715
  13. Sapolsky, R. M., Why stress is bad for your brain. Science, 273, 749-750 (1996) https://doi.org/10.1126/science.273.5276.749
  14. Shapiro, S., Kubek, M., Sanders, S., Durbib, S., Goodwin, S., and Javed, T., Regional changes in central nervous system thyrotropin-releasing hormone after pentylenetetrazol-induced seizures in dogs. Neurosurgery, 31, 935-939 (1992) https://doi.org/10.1227/00006123-199211000-00017
  15. Vallee, M., Rivera, J. D., Koob, G. F., Purdy, R. H., and Fitzgerald, R. L., Quantification of neurosteroids in rat plasma and brain following swim stress and allopregnanolone administration using negative chemical ionization gas chromatography/mass spectrometry. Anal. Biochem., 287, 153-166 (2000) https://doi.org/10.1006/abio.2000.4841
  16. Yun, S. J., Lee, D. J., Kim, M. O., Jung, B., Kim, S. O., Sohn, N. W., and Lee, E. H., Reduction but not cleavage of poly(ADPribose) polymerase during stress-mediated cell death in the rat hippocampus. Neuroreport, 14, 935-939 (2003) https://doi.org/10.1097/00001756-200305230-00006