Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Quercitrin Gallate Down-regulates Interleukin-6 Expression by Inhibiting Nuclear Factor-kB Activation in Lipopolysaccharide-stimulated Macrophages

  • Min, Kyung-Rak (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Byung-Hak (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Chang, Yoon-Sook (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kim, Young-Soo (College of Pharmacy & Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2006.06.01
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Abstract

Quercitrin gallate was previously isolated from Persicaria lapathifolia (Polygonaceae) as an inhibitor of superoxide production. In the present study, quercitrin gallate was found to inhibit interleukin (IL)-6 production in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7 with an $IC_{50}$ value of $63\;{\mu}M$. Furthermore, quercitrin gallate attenuated LPS-induced synthesis of IL-6 transcript but also inhibited LPS-induced IL-6 promoter activity, indicating that the compound could down-regulate IL-6 expression at the transcription level. Since nuclear factor (NF)-kB has been shown to play a key role in LPS-inducible IL-6 expression, an effect of quercitrin gallate on LPS-induced NF-kB activation was further analyzed. Quercitrin gallate exhibited a dosedependent inhibitory effect on LPS-induced nuclear translocation of NF-kB without affecting inhibitory kB (IkB) degradation, and subsequently inhibited LPS-induced NF-kB transcriptional activity in macrophages RAW 264.7. Taken together, quercitrin gallate down-regulated LPS-induced IL-6 expression by inhibiting NF-kB activation, which could provide a pharmacological potential of the compound in IL-6-related immune and inflammatory diseases.

Keywords

References

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