Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

In Vitro Effects of Water and Methanol Extracts of Melittia inouei on Cytokine Production

유리나방 유충 추출물이 비장 세포로부터 Cytokine 분비에 미치는 효과

  • Lee, Hyun-Ah (The Cancer center, Samsung Medical Center, School of Medicine, Sungkyunkwan University) ;
  • Shon, Hye-Jin (The Cancer center, Samsung Medical Center, School of Medicine, Sungkyunkwan University) ;
  • Yang, Young-Taek (Jejudo Agriculture Research & Extension Service) ;
  • Kim, Kyu-Don (Hong-Gum Company) ;
  • Park, Hae-Cheol (Department of Agricultural Biology, National Institute of Agricultural Science & Technology, RDA) ;
  • Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science & Technology, RDA) ;
  • Hwang, Suk-Jo (Department of Agricultural Biology, National Institute of Agricultural Science & Technology, RDA) ;
  • Ahn, Mi-Young (Department of Agricultural Biology, National Institute of Agricultural Science & Technology, RDA)
  • 이현아 (성균관의대 삼성서울병원 암센터) ;
  • 손혜진 (성균관의대 삼성서울병원 암센터) ;
  • 양영택 (난지농업연구소) ;
  • 김규돈 (주식회사 홍금) ;
  • 박해철 (농업과학기술원 농업생물부) ;
  • 황재삼 (농업과학기술원 농업생물부) ;
  • 황석조 (농업과학기술원 농업생물부) ;
  • 안미영 (농업과학기술원 농업생물부)
  • Published : 2006.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Melittia inouei (Yuri Nabang) larvae are used as a crude drug in East Asia for treating stomach cancer and inflammation, and currently reared as a pharmaceutical insect in Jejudo, Korea. This study evaluated the immuno-modulating activity of these extracts, by determining the level of, cytokine production from mouse splenocytes stimulated with the extracts. The Melittia inouei larvae extracts did not induce the splenocyte proliferation. On the other hand, they stimulated the splenocytes to produce cytokines such as $TNF-{\alpha}$, whereas they did not stimulate IL10, IL12 or $IFN-{\gamma}$. The aqueous portion of its plant (Tri-chosanthis kirilowii) extract (sap) was found to be a potent inducer of NO production from the CPAE cells. However, it showed weak inhibitory effects on vascular endothelial growth factor (VEGF) production from splenocytes. These data suggests that a Melittia inouei larvae extract immune modulatory activity in cytokine prodcutions such as $TNF-{\alpha}$ and VEGF which might be related its anticancer effect.

Keywords

References

  1. Kim, J. G. (1996) Traditional drugs of the East. pp. 82. YoungLimSa Press, Seoul
  2. 장기창, 김성철, 송은영, 김공호, 박기훈 (2004) 2004년 보고서, 난지 지원식물 상품화 및 기능성물질 탐색, 93-115, 농촌진흥청 난지농업연구소, 제주
  3. Young, H. and Hardy K. (1995) Role ofinterferone-gamma in immune cell regulation. J. Leukoc. Biol., 58, 373-381 https://doi.org/10.1002/jlb.58.4.373
  4. Thomson A. (1994) The Cytokine Handbook 2nd Ed., Academic Press
  5. Ruddon, R. W. (1987) Cancer Biology, Biology of tumor metastasis, 443-467, Oxford University Press, New York
  6. Lee, S. J. and Lee, H. K. (2005) Sanguiin H-6 blocks endothelial cell growth through inhibition of VEGF binding receptor. Arch. Pharm. Res., 28, 1270-1274 https://doi.org/10.1007/BF02978211
  7. Carmeliet, P. (2005) VEGF as a key mediator of angiogenesis in cancer. Oncology, 69(suppl 3), 4-10 https://doi.org/10.1159/000088478
  8. Weston, S. A. and Parish, C. R. (1990) New fluorescent dyes for lymphocyte migration studies : Analysis by flow cytometry and fluorescence microscopy, J. Imuunol. Method, 133, 87-97 https://doi.org/10.1016/0022-1759(90)90322-M
  9. Hasbold, J., Gett, A. V., Rush, J. S., Deenick, E., Avery, D., Jun, J. and Hodgkin, P. D. (1999) Quantitative analysis of lymphocyte differentiation and proliferation in vitro using carboxyfluorescein diacetate succinimidyl ester. Immunol. Cell Bioi. 77, 516-522 https://doi.org/10.1046/j.1440-1711.1999.00874.x
  10. Nims, R.W., Darbyshire, J.F., and Saavedra, J.E. (1995) Colorimetric methods for the determination of nitric oxide concentration in neutral aqueous solutions. Methods, 7, 48-54 https://doi.org/10.1006/meth.1995.1007
  11. Suh, K. S. Muto, M., Gerdes, M., Crutchley, J. M., Mutoh,T., Edwards, L. E., Dumont, R. A. Sodha, P., Cheng, C., Glick, A. and Yuspa, S. H. (2005) Antisense suppression of the chloride intracellular channel family induces apoptosis, enhances tumor necrosis factor a-induced apoptosis, and inhibits tumor growth. Cancer Res., 65, 562-571
  12. Buanecl, H. L., D'Anna', R., Lachgarl, A., Zagury', Bemard', J., lttele, D., d'Alessio', P., Hallez, S., Giannouli, C., Burny, A., Bizzini', B. GalW, R.C., and Zaguryl, D. (1999) HPV-16 E7 but not E6 oncogenic protein triggers both cellular immunosuppression and angiogenic processes. Biomed & Pharmacother, 53, 424-31 https://doi.org/10.1016/S0753-3322(99)80122-X
  13. Strauss, L., Volland, D., Kunkel, M. and Reichert, T. E. (2005) Dual role of VEGF family members in the pathogenesis of head and neck cancer (HNSCC): Possible link between angiogenesis and immune tolerance. Med Sci. Manit, 1, BR280-2921
  14. Kim, S. C., Park, S. Y., Hyoun J. H., Lee Y. K., Park, D. B. Kang S. Y., Yoo, E. S. and Kang, H. K. (2003) Induction of apotosis by extract of Irichosanthes kirilpwii var.japonica in HL-60 leukemia cells. Yakhak Hoeji, 319-324