Elastase and Hyaluronidase Inhibition Activities of Phlorotannins Isolated from Ecklonia cava

감태(Ecklonia cava)에서 분리한 Phlorotannin 성분의 Elastase 저해활성 및 Hyaluronidase 저해활성

  • Bu, Hee-Jung (Department of Chemistry, Cheju National University) ;
  • Ham, Young-Min (Department of Chemistry, Cheju National University) ;
  • Kim, Jung-Mee (Department of Chemistry, Cheju National University) ;
  • Lee, Sun-Joo (Department of Chemistry, Cheju National University) ;
  • Hyun, Jin-Won (Department of Medicine, Cheju National University) ;
  • Lee, Nam-Ho (Department of Chemistry, Cheju National University)
  • Published : 2006.06.30

Abstract

Six phlorotannins were isolated from Ecklonia cava, a brown alga belonging to Alariaceae, collected offshore Jeju Island. Among the phlorotannins, dieckol showed strong elastase inhibition and hyaluronidase ingibition.

Keywords

References

  1. Tsuji, N., Moriwaki, S., Suzuki, Y., Takemura, Y., and Imokawa, G. (2001) The role of elastase secreted by fibroblasts in wrinkle formation; implication through selective inhibition of elastase activity. Photochem. and Photobiol. 74: 283-287 https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  2. 생리활성 정밀화학 기술개발 로드맵 (2002). 229-322. 산업자원부. 서울
  3. Menzel, E.J. and Farr, C. (1998) Hyaluronidase and its substrate hyaluronan: biochemistry, biological activities and therapeutic uses. Cancer Letters. 131: 3-11 https://doi.org/10.1016/S0304-3835(98)00195-5
  4. Kakegawa, H., Matsumoto, H., and Satoh, T. (1992) Inhibitory effects of some natural products on the activation of hyaluronidase and their anti-allergic action. Chem. Pharm. Bull. 40: 1439-1442A https://doi.org/10.1248/cpb.40.1439
  5. Kim, J.A., Lee, J.M., Shin, D.B., and Lee, N.H. (2004) The antioxidant activity and tyrosinase inhibitory activity ofphlorotannins in Ecklonia Cava. Food Sci. Biotechnol. 13: 476-480
  6. Kang, H.S., Chung, H.Y., Jung, J.H., Son, B.W., and Choi, J.S. (2003) A new phlorotannin from the brown alga EckIonia stolonifera. Chem. Pharm. Bull. 51: 1012-1014 https://doi.org/10.1248/cpb.51.1012
  7. Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Kido, M., Mori, H., Nakayama, Y., and Takahashi, M. (1989) Structure of an anti-plasmin inhibitor, eckol, isolated from the brown alga Ecklonia kurome OKAMURA and inhibitory activities of its derivatives on plasma plasmin inhibitors. Chem. Pharm. Bull. 37: 349-353 https://doi.org/10.1248/cpb.37.349
  8. Nakamura, T., Nagayama, K., Uchida, K., and Tanaka, R. (1996) Antioxidant activity of phlorotannins isolated from the brown alga Eisenia bicylis. Fisheries Science. 62: 923-926 https://doi.org/10.2331/fishsci.62.923
  9. Kang, H.S., Chung, H.Y., Kim, J.Y., Son, B.W., Jung, H.A., and Choi, J.S. (2004) Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch. Pharm. Res. 27: 194-198 https://doi.org/10.1007/BF02980106
  10. Kang, K., Park, Y., Hwang, H.J., Kim, S.H., Lee, J.G., and Shin, H.C. (2003) Antioxidative properties of brown algae polyphenolics and their perspectives as chemopreventive agents against vascular risk factors. Arch. Pharm. Res. 26: 286-293 https://doi.org/10.1007/BF02976957
  11. Ahn, M.J., Yoon, K.D., Min, S.Y., Lee, J.S., Kim, J.H., Kim, T.G., Kim, S.H., Kim, N.K., Huh, H., and Kim, J.W. (2004) Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol. Pharm. Bull. 27: 544-547 https://doi.org/10.1248/bpb.27.544
  12. James, A.E.K., Timothy, D.W., and Gorden, L. (1996) Inhibition of human leukocyte and porcin pancreatic elastase by homologues of bovine pancreatic tyrosin inhibitors. Biochemistry. 35: 9090-9096 https://doi.org/10.1021/bi953013b
  13. 류재천, 박종세, 송윤선 (1990) 항알러지 및 항염증 작용의 시험관내 1차 스크리닝법. 한국과학기술연구원 도핑컨트롤센터. 한국생화학회
  14. Kang, K.A., Lee, K.H., Chae, S.W., Koh, Y.S., Yoo, B.S., Kim, J.H., Ham, Y.M., Baik, J.S., Lee, N.H., and Hyun, J.W. (2005) Triphlorethol-A from Ecklonia cava protects V79-4 lung fibroblast against hydrogen peroxide induced cell damage. Free Radical Research 39: 883-892 https://doi.org/10.1080/10715760500161165
  15. Shibata, T., Fujimoto, K., Nagayama, K., Yamaguchi, K., and Nakamura, T. (2002) Inhibitory activity of brown algal phlorotannins against hyaluronidase. Int. J. Food Sci. Tech. 37: 703-709 https://doi.org/10.1046/j.1365-2621.2002.00603.x
  16. Stem, J.L., Hagerman, A.E., Steinberg, P.D., and Mason, P.K. (1996) Phlorotannin-protein interactions. J. Chem. Ecology 22: 1877-1899 https://doi.org/10.1007/BF02028510