Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and Identification of an Antioxidant Substance from Ethanol Extract of Wild Grape (Vitis coignetiea) Seed

머루종자 에탄올 추출물로부터 항산화활성물질 분리 및 동정

  • Kim, Nan-Young (School of biotechnology and Bioengineering, Kangwon National University) ;
  • Choi, Jae-Ho (School of biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Young-Guk (School of biotechnology and Bioengineering, Kangwon National University) ;
  • Jang, Mi-Young (Department of Food Science & Technology and Functional Food Research Center, Chonnam National University) ;
  • Moon, Jea-Hak (Department of Food Science & Technology and Functional Food Research Center, Chonnam National University) ;
  • Park, Geun-Hyung (Department of Food Science & Technology and Functional Food Research Center, Chonnam National University) ;
  • Oh, Deog-Hwan (School of biotechnology and Bioengineering, Kangwon National University)
  • 김난영 (강원대학교 바이오산업공학부) ;
  • 최재호 (강원대학교 바이오산업공학부) ;
  • 김영국 (강원대학교 바이오산업공학부) ;
  • 장미영 (전남대학교 응용생물공학부 및 기능성식품연구센터) ;
  • 문제학 (전남대학교 응용생물공학부 및 기능성식품연구센터) ;
  • 박근형 (전남대학교 응용생물공학부 및 기능성식품연구센터) ;
  • 오덕환 (강원대학교 바이오산업공학부)
  • Published : 2006.02.01
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Abstract

Antioxidant compound(s) were identified from the ethanol extract of wild grape (Vitis coignetiea) seed. Organic solvent fractions of n-hexane, chloroform, ethyl acetate and butanol were obtained from the ethanol extract of wild grape seed, among which ethyl acetate fraction showed the strongest reducing power. Ethyl acetate fraction was further purified through ODS column chromatography and HPLC, and isolated antioxidative active compound was identified through $^1H-NMR$ as (+)-catechin (52.7 g/100 g). (+)-Catechin and ethyl acetate fraction both showed approximately 80% scavenging effect. These results indicated (+)-catechin in the ethyl acetate fraction synergetically interacts with unknown antioxidative compound(s).

본 연구는 머루종자 에탄올 추출물로부터 항산화 활성물질을 분리 동정하고 분리 물질의 항산화 활성을 검토하기 위하여 수행되었다. 머루종자 70% 에탄올 추출물로부터 hexane, chloroform, ethyl acetate, butanol, 및 수용액으로 순차 용매분획하여 그들의 항산화 활성 및 환원력을 비교한 결과, ethyl acetate층이 가장 강한 활성을 나타냈으며, 각 획분의 TLC-DPPH 분석에서도 ethyl acetate층에서 현저히 높은 항산화활성을 보인 화합물의 존재가 확인되었다. 이에 ethyl acetate층을 대상으로 ODS column chromatography 및 HPLC를 이용하여 활성본체를 단리하고, 1H-NMR 분석을 통하여 (+)-catechin으로 동정하였다. 머루종자 중의 (+)-catechin 함량은 52.7 mg/100 g으로 확인되었으며, (+)-catechin이 머루종자 중의 주요한 항산화 물질로서 사료되었다. 한편, 같은 농도$(15\;{\mu}g/{\mu}L)$에서 (+)-catechin과 ethyl acetate 분획물의 DPPH radical 소거활성이 약 80%로 비슷한 결과를 나타낸 것으로 보아 ethyl acetate 분획물에는 (+)-catechin이외의 미확인 물질들이 서로 상승작용을 하여 강한 항산화 활성을 나타내는 것으로 판단되었다.

Keywords

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