Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of Dietary Fats and Tomato on the Immune Functions of BALB/c Mice

식이지방과 토마토가 BALB/c 마우스의 면역활성에 미치는 영향

  • Published : 2006.04.01
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Abstract

Effects of dietary fat and tomato on immune functions were investigated using BALB/c mice. Sixty male BALB/c mice weighing 16-17g were divided into two dietary groups. Control group was fed experimental chow (AIN-76 purified diet) supplemented with saturated fat (beef tallow) and unsaturated fat (safflower oil) at 5% level (weight basis), while treatment group was fed chow added with 0.5% (dry matter basis) of tomato (moisture content 95%) for 10 weeks. Organ weights, delayed-type hypersensitivity test, plaque-forming cell test, agglutination test, differential white cell count, and histological examination were performed at 4th, 7th, and 10th week after dietary treatment. Weight of spleen and spleen index of tomato group increased with increasing experimental period. Thymus index of tomato group was significantly higher than that of control group at 7 and 10 weeks (p<0.05). In delayed hypersensitivity test at 4th, 7th, and 10th week after dietary treatment, tomato group showed more significant increase in footpad swelling reaction 24 hr after challenge than control group (p<0.05). In plaque-forming cell test, tomato group fed 7 weeks increased more significantly than control group, while no significant difference was found between two groups at 10 weeks. Agglutination test decreased with increasing experimental period; tomato group at 10 weeks was higher than control group in antibody response to SRBC. In tomato group, Iympocyte percentage was slightly higher than that of control group, and spleen tissue showed active immune reaction caused by severe proliferation of white pulp.

본 연구는 식이지방(우지, 잇꽃유)을 급여한 마우스에게 토마토가 면역활성에 미치는 영향을 평가하기 위하여 수행하였다. AIN-76 diet에 포화지방(beef tallow)과 불포화지방(safflower oil)을 각각 5%씩 중량비로 혼합한 식이를 대조군으로 하였고, 실험군은 토마토(수분함량, 95%)를 고려하여 건물기준으로 0.5%를 첨가한 실험식이를 제조하여 BALB/c 마우스에게 10주 동안 급여하였다. 지연성 과민반응검사는 4, 7, 10주에, 항체생산 세포수측 정은 7, 10주에 실시하였다. 혈액은 심장천자(heart puncture)로 채취하였으며 저장된 혈청은 양적혈구(SRBC)에 대한 적혈구응집소가측정(AGG test)에 이용하였다. 각각의 장기는 적출하여 칭량하였고, DTH test후 적출한 비장을 대상으로 세포조직학적 검사를 실시하였다. 토마토군은 대조군에 비해 4주와 10주의 비장계수(spleen index)와 흉선계수(thymus index)가 통계적으로 높았다(p<0.05). 양적혈구에 대한 지연성 과민반응검사에서 토마토군은 4주, 7주, 10주 모두 24시간의 반응값이 대조군 보다 통계적으로 유의하게 높았다(p<0.05). 토마토군은 3시간은 7주, 24시간은 4주, 48시간은 4주와 10주에서 대조군에 비해 통계적인 유의성이 있었다(p<0.05). 항체생산 세포수는 식이급여 7주에 토마토군이 대조군보다 통계적인 차이가 나타난 반면 10주는 차이가 없었다. 양적혈구에 대한 응집소가는 식이의 급여기간이 길어질수록 감소하였는데, 토마토군이 대조군에 비해서 특히 10주의 응집소가는 유의성이 있었다. 임파구(Iymphocyte)는 토마토군이 대조군보다 다소 증가하였고, 비장조직 검사결과 토마토군은 백색수(white pulp)가 심하게 증식되어 활발한 면역반응을 보였다.

Keywords

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