Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibitory Effects of Fungal Metabolites Isolated from Foodstuffs on the Growth of Human Cancer Cell Lines

식품유래 곰팡이 대사산물의 항암효과

  • Im, Hyo-Gwon (Department of Food Science and Technology, Keimyung University) ;
  • Yu, Mi-Hee (Department of Food Science and Technology, Keimyung University) ;
  • Chung, Duck-Wha (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University)
  • 임효권 (계명대학교 식품가공학 전공) ;
  • 유미희 (계명대학교 식품가공학 전공) ;
  • 정덕화 (경상대학교 응용생명과학부) ;
  • 이인선 (계명대학교 전통미생물자원개발 및 산업화연구(TMR) 센터)
  • Published : 2006.04.01
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Abstract

Inhibitory effects of fungal metabolites isolated from foodstuffs on growth of human cancer cell lines were evaluated. Isolated strains were divided into four classes based on color (aerial, reverse), shape, and growth speed. Fungal metabolites extracted with ethyl acetate were investigated for their growth inhibition on six kinds of human cancer cells by MTT assay. Ethyl acetate extract showed high growth inhibition against all cancer cells tested, with D4 exhibiting the strongest growth inhibition effects against Kato III, AGS, Hepa1c1c7, and MDA-MB-231 cancer cells. These results suggest ethyl acetate extract from fungal metabolites as effective natural cancer therapeutic material.

본 논문은 새로운 항암소재를 검색하기 위하여 식품으로부터 곰팡이를 분리하고 그 대사산물을 이용하여 수종의 암세포주의 생육을 대한 억제 물질을 탐색하고자 하며, 이를 바탕으로 새로운 항생물질 연구의 기초자료를 확보하고 결과물의 응용도를 모색하고자 실험을 수행하였다. 식품으로부터 총 72종의 곰팡이를 분리 하였고, 전면 및 후면의 색, 모양. 성장속도에 따라 4가지 group로 나누어 실험에 사용하였다. 그리고 그 대사산물(배양무, 균체)을 ethly acetate로 추출하여, 총 6종의 암세포주에 대하여 MTT assay을 실험하였다. 그 ruf과 A, B, C, D group 중 D group에서 가장 높은 증식 억제를 확인 하였으며, D4의 증식억제 효과가 가장 높음을 확인하였다. 위암 세포주인 KATO III, AGS, SUN-668에 대한 증식억제 효과는 30-98%로 나타났으며, 간암 세포주인 Hepa1c1c7, HepG2에는 10-98%, 유방암 세포주인 MDA-MB-231에 대해서는 25-90%의 증식억제 효과를 확인하였다. 배양물과 균체의 항암성은 개체에 따라 약간의 차이는 있으나, 배양물이 대부분 우수한 것으로 확인되었다.

Keywords

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