Study on the Improvement of Quality in Jeung-pyun Prepared with Lactic Bacteria Having High Dextransucrase Activity as Starters

Dextransucrase 고활성 젖산균을 이용한 스타터 증편의 품질개선에 관한 연구

  • Lee, A-Young (Department of Food & Nutrition, Sungshin Women's University) ;
  • Park, Ju-Yeon (Department of Food & Nutrition, Sungshin Women's University) ;
  • Hahn, Young-Sook (Department of Food & Nutrition, Sungshin Women's University)
  • 이아영 (성신여자대학교 식품영양학과) ;
  • 박주연 (성신여자대학교 식품영양학과) ;
  • 한영숙 (성신여자대학교 식품영양학과)
  • Published : 2006.06.01

Abstract

Twenty six strains of lactic acid bacteria were isolated from Jeung-pyun batter, among which Tetragenococcus halophilus 1-12 showed highest dextransucrase activity at 36.95 DSU/mg, followed by T. halophilus 5-8 (36.87 DSU/mg protein), T. halophilus 2-12 (32.66 DSU/mg protein), T. halophilus 3-3 (31.43 DSU/mg protein), T. halophilus 3-1 (30.73 DSU/mg protein), T. halophilus 5-12 (29.43 DSU/mg protein), and Leuconostoc mesenteroides subsp mesenteroides 2-9 (28.5 DSU/mg protein). L. mesenteroides subsp mesenteroides 2-9, T. halophilus 1-12, and L. mesenteroides subsp dextranicum 5-13, were selected as starters (0.1, 0.5, and 1.0%) for Jeung-pyun manufacturing. Specific volume of Jeung-pyun added with 1.0% L. mesenteroides subsp dextranicum 5-13 was highest at 2.00, and 1.0% T. halophilus 1-12-added Jeung-pyun was lowest at 033. Cross-sectional observation of Jeung-pyun showed Jeung-pyun added with 0.5% L. mesenteroides subsp mesenteroides 2-9 was uniformly formed. Number of air holes of Jeung-pyun increased with increasing amount of added T. halophilus 1-12. Increasing addition of L. mesenteroides subsp dextranicum 5-13 resulted in more uniform air holes and volumes. Hardness, gumminess, and chewiness of Jeung-pyun added with 0.5 and 2.0% L. mesenteroides subsp dextranicum 5-13 decreased significantly in compasion to control groups. Jeung-pyun added with 0.5% L. mesenteroides subsp dextranicum 5-13 showed excelIent overall sensory desirability ranking of 8.500.

26균주의 젖산균을 증편 반죽에서 분리하여 dextransucrase 활성을 측정할 결과 T. halophilus 1-12가 36.95 DSU/mg으로 가장 높았다. 이 중 Leu. mesenteroides subsp mesenteroides 2-9, T. halophilus 1-12, Leu. mesenteroides subsp dextranicum 5-13을 선별하여 starter로 이용하였다. 증편의 점도변화를 관찰한 결과 Leu. mesenteroides subsp mesenteroides 2-9를 첨가한 증편은 발효 적기인 2시간째에 첨가량이 많을수록 점도가 높아졌고 T. halophilus 1-12를 첨가한 증편의 경우는 첨가량에 따라 차이 없이 증편의 점도가 증가하였다. Leu. mesenteroides subsp dextranicum 5-13를 첨가한 증편 반죽의 경우에는 첨가량에 차이 없이 starter 균을 첨가하지 않은 증편 반죽의 발효패턴과 같은 점도 변화를 보였다. 증편반죽의 pH는 Leu. mesenteroides subsp mesenteroides 2-9 와 T. halophilus 1-12를 첨가한 경우 첨가량이 증가할수록 초기 pH도 낮았으며 발효가 진행되면서 계속 감소되는 경향을 보였다. 그러나 Leu. mesenteroides subsp dextranicum 5-13의 경우에는 첨가량에 따른 유의적인 차이가 없이 발효가 진행되면서 감소하는 패턴을 보였다. 증편의 비체적은 Leu. mesenteroides subsp dextranicum 5-13 1.0% 첨가한 군이 2.00으로 가장 높았고, T. halophilus 1-12를 1.0% 첨가한 군이 0.33으로 가장 낮았다. 증편의 단면을 관찰한 결과 Leu. mesenteroides subsp mesenteroides 2-9를 0.5 % 첨가한 것이 균일하였고, T. halophilus 1-12는 첨가량이 많을수록 기공이 거칠고 불규칙하였다. 반면 Leu. mesenteroides subsp dextranicum 5-13를 첨가한 군은 첨가량이 많을수록 기공이 균일하고 부피가 증가한 것을 볼 수 있었다. Texture는 Leu. mesenteroides subsp dextranicum 5-13를 0.5%, 1.0% 첨가한 증편이 hardnesubsp과 gumminesubsp, chewinesubsp가 대조군에 비해 매우 유의적으로 감소하였다. 관능검사를 실시한 결과 Leu. mesenteroides subsp dextranicum 5-13 0.5%를 첨가한 시료가 전체적인 기호도에서 8.500을 받아 매우 우수한 것으로 나타났다.

Keywords

References

  1. Hahn YS. Oh JY. 1999. Dextransucrase activity of Leuconostoc sp. strains isolated from Kimchi. Korean J. Appl. Microbiol. Biotechnol. 27: 86-89
  2. Bucke CL. Deavin CJ. Lawson, Pindar DF. The production of industrially important bacterial polysaccharides. Biochem. Soc. Trans. 3: 844-847 (1975) https://doi.org/10.1042/bst0030844
  3. Calley BJ. The extracellular polysaccharide, pullulan, produced by Aureobasidium pullulans: A relationship between elaboration rate and morphology. J. Gen. Microbiol. 120: 265-268 (1980)
  4. Cotrell IW. Industrial potential of fungal and bacterial polysaccharides. ACS Symp. Ser. 126: 251-270 (1980)
  5. Lowry OH. Rosebrough NJ. Farr AL. Randall RJ. Protein measurment with the folin phenol reagent. Biol. Chem. 193: 265-275 (1951)
  6. Yoon MH, Koo YM, Mechanism of dextran synthesis by dextransucrase. Korean J. Biotechnol. Bioeng. 9: 1-7 (1994)
  7. Jeanes A. Dextrans. Encycl. Polym. Sci. Technol. 4: 805-824 (1966)
  8. Eom HJ, Seo DM, Yoon HS, Lee HB, Han NS. Strain selection of psychrotrophic Leuconostoc mesenteroides producing a highly active dextransucrase from Kimchi. Korean J. Food Sci. Technol. 34: 1085-1090 (2002)
  9. An S, Lee KA, Kim KJ. Quality characteristics of Jeung-pyun according to the leavening agents. Korean J. Human Ecol. 5: 48-61 (2002)
  10. Shin KS, Woo KJ. Study on the dextran and the inside structure of Jeung-pyun of adding soybean. J. East Asian Soc. Diet. Life 11: 121-130 (2001)
  11. Lee HE, Study on the structure of Jeung-pyun (rice cake) and microbial dextransucrase activities. MS thesis. Sungshin Women's Univ, Seoul, Korea (2004)
  12. Moon HJ, Chang HG, Mok CK. 1999. Selection of lactic starter for the improvement of Jeungpyun manufacturing process. Korean J. Food Sci. Technol. 31: 1141-1146
  13. Lee EA, Woo KJ. Study on the dextran and the inner structure of Jeung-Pyun (Korean rice cake) on adding oligosaccharide. J. East Asian Soc. Diet. Life 12: 38-46 (2002)