Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Effect of Antihypertensive Peptides Originated from Various Marine Proteins on ACE Inhibitory Activity and Systolic Blood Pressure in Spontaneously Hypertensive Rats

어육단백질로부터 분리된 항고혈압 펩타이드가 ACE 저해활성과 본태성 고혈압쥐의 혈압에 미치는 영향

  • Published : 2006.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the effect of antihypertensive peptides originating from marine proteins on ACE inhibitory activity and systolic blood pressure in spontaneously hypertensive rats (SHR). Sixteen male SHR (SHR/NCrj) weighing approximately 270 g were randomly divided into few experimental groups based on diet: C (control), A (anchovy), P (pollack) and M (mackerel). The final body weights of P and M groups were higher, than those of C and A groups, but difference was not significant. Average reference blood pressure (RBP) was 224 mmHg at 12 weeks old. Compared with RBP, final systolic blood pressure of the marine peptide oops after 28 days of feeding with anchovy, pollack and mackerel fractions by gavage was decreased by 9.0% (A), 10.2% (P) and 14.3% (M), respectively, but was not different in C. Especially, final blood pressure of M was lower by 32 mmHg than RBP. These results suggested that peptide originated from mackerel hydrolysate was considered to have an antihypertensive fraction as effective lowering of blood pressure in SHR.

본 연구는 항고혈압 활성이 있는 어육 단백질의 효소 가수분해물로부터 얻어진 peptide분말을 실험동물인 본태성 고혈압쥐(SHR)에게 음용수로 공급하여 혈압강하에 미치는 영향을 알아보기 위해 실시하였다. in vitro에서 멸치, 명태 그리고 고등어 가수분해물 500 ${\mu}g$을 첨가하여 각각 75, 81 그리고 35%의 ACE 저해활성을 보였던 펩타이드 분말 0.4167%을 희석하여 SHR에게 음용수로 공급한 후 생리적 변화를 관찰하기 위해 매 1주일 마다 총 섭취한 사료의 무게와 음수량을 측정하였다. 실험기간 중 SHR의 일당증체량은 명태(C)와 고등어처리군(D)이 각각 1.26 및 1.36 g으로 A군(0.78 g)과 B군(0.77 g) 보다 높았지만 유의적인 차이는 없었다. 사료섭취량은 $16.85{\sim}19.93$ g/day으로 거의 차이가 없었던 것으로 나타났다. 생후 13주령된 SHR를 대상으로 실험개시 전 측정한 기준혈압(RBP)은 실험군에서 평균 224mmHg 로 큰 차이가 없이 비슷하였다 어육 펩타이드 음용군(멸치, 동태, 고등어)은 경시적으로 감소경향을 나타냈으며 실험군 중 D군(고등어)의 수축기혈압은 투여 후 7일에 208mmHg로 측정되어 RBP 보다 15 mmHg가 감소하였고, 이 후 비슷한 수준을 유지하다가 28일에 191 mmHg로 실험군 중 가장 낮았다. C군(명태)은 투여 후 14일에 214mmHg로 측정되어 11 mmHg가 감소하였고, 28일에 202 mmHg로 나타났다. B군(멸치)은 투여 후 7일까지는 기준혈압(223 mmHg)과 비슷하였고, 이후 28일까지 203 mmHg로 일정한 수준을 유지하였다. 어육 펩타이드 대신 생수음용 대조군(A)은 실험기간 중 225-216 mmHg로 유의한 차이가 나타나지 않았다. 기준혈압(0일) 대비 28일의 혈압 감소치는 어육 펩타이드(멸치, 동태, 고등어) 음용군에서 각각 20, 23, 32 mmHg로 RBP대비 혈압감소율은 각각 9.0, 10.2, 14.3%로 나타났다.

Keywords

References

  1. Kannel WB, Dawber TR, Sorlie P. Components of blood pressure and risk of athrothrombotic brain infarction. Stroke 7: 327-331 (1976) https://doi.org/10.1161/01.STR.7.4.327
  2. Society of Oriental Medicine. In: The Modem Oriental Medicine. Hakchang-Sa, Seoul, Korea. pp. 446-447 (1993)
  3. Yukio Y, Yasuo N, Motoki T, Masayuki M, Masahiro K, Keigo F, Ryoichi H , Kohtaro K. Common cellular deposition to hypertension and atherosclerosis. J. Hypertens. 2: 213-215 (1984)
  4. Okamoto K, Aoki K. Development of a strain of spontaneously hypertensive rats. Jpn. Circ. J. 27: 282-293 (1963) https://doi.org/10.1253/jcj.27.282
  5. Okamoto K, Tabei R, Fukushima M, Mosaka S, Yamori Y, Ichijima K, Haebara H, Matsumoto M, Maruyama T, Suzuki Y, Tamegai M. Further observations of the development of a strain of spontaneously hypertensive rats. Jpn. Circ. J. 30: 703-716 (1966) https://doi.org/10.1253/jcj.30.703
  6. Kirkendall WM, Nottebohm GA. Hypertension physiopathology and treatment. pp. 674-692. In: Essential Hypertension. McGraw-Hill. New York, USA (1977)
  7. Goldberg I. Designer foods, pharmafoods, nutraceuticals. pp. 249-256. In: Functional Foods. Champman & Hall. Inc., New York, USA (1994)
  8. Korhonen H, Pilhlanto-Leppala A, Rantamaki P, Tupassela T. Impact of processing on bioactive proteins and peptides. Trends Food Sci. Tech. 9: 307-319 (1998) https://doi.org/10.1016/S0924-2244(98)00054-5
  9. Do JR. Separation and purification of Angiotensin I-converting enzyme inhibitory peptide from mackerel. J. Korean Fish. Soc. 33: 153-157 (2000)
  10. AOAC. Official Method of Analysis of AOAC Intl. 15th Edition Edited by Kenneth Helrich. Association of Official Analytical Chemists. Washington DC, USA (1990)
  11. Cushman DW, Cheung HS. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648 (1971) https://doi.org/10.1016/0006-2952(71)90292-9
  12. American Institute of Nutrition. Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. J. Nutr. 107: 1340-1348 (1977) https://doi.org/10.1093/jn/107.7.1340
  13. Choe M, Tae WC, Kim JD. Effect of dietary fibers on changes of blood pressure and Na balance in spontaneous hypertensive rats. Korean J. Nutr. 24: 40-47 (1991)
  14. SAS Institute, Inc. SAS User's Guide. Statistical Analysis Systems Institute, Cary, NC, USA (1992)
  15. Kong CS, Bak SS, lung KO, Kil JH, Lim SY, Park KY. Antimutagenic and anticancer effects of salted mackerel with various kinds of salts. J. Korean Fish. Soc. 38: 281-285 (2005) https://doi.org/10.5657/kfas.2005.38.5.281
  16. Jin YH, Kwon OC, Sung NJ, Shin JH, Kang MJ. Effect of garlic on quality of low salted anchovy. Cul. Res. 11: 49-70 (2001)
  17. Oh KS. Changes in lipid components of pollack during sun-drying. Korean J. Food Sci. Technol. 26: 123-126 (1994)
  18. Lee TG, Park YB, Park DC, Yeum DM, Kim IS, Gu YS, Park YH, Kim SB. Angiotensin converting enzyme inhibitory activity in enzymatic hydrolysates of anchovy muscle protein. J. Korean Fish Soc. 31: 875-881 (1998)
  19. Jang A, Cho YJ, Lee JI, Shin JH, Kim IS, Lee M. The effect of beef peptide on blood pressure and serum lipid concentration of spontaneously hypertensive rat (SHR). Korean J. Anim. Sci. Technol. 46: 107-114 (2004) https://doi.org/10.5187/JAST.2004.46.1.107