Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of Acanthopanacis cortex Water Extract on Antioxidative Activity, Lipid Profile and Epidermal Thickness in DNCB-induced Allergic Contact Dermatitis Animal Model

오가피열수추출물이 DNCB로 유도된 알레르기성 접촉피부염 흰쥐의 지질대사, 항산화능 및 표피회복능에 미치는 영향

  • 김윤희 (대구대학교 공과대학 식품영양학과) ;
  • 박영숙 (대구대학교 공과대학 식품영양학과)
  • Published : 2006.10.01
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Abstract

The effects of Acanthopanacis cortex water extract on allergic contact dermatitis (ACD) in Sprague-Dawley male rats treated with 2.5% 1-chloro-2,4-dinitrochlorobenzene (DNCB) on the dorsal skin were evaluated. The concentration of TBARS in plasma and liver was increased in ACD rats and was significantly decreased in Acanthopanacis cortex fed group (ACFG) compared to CG (control group). Serum Ig E level was significantly increased in CG compared to normal group, while that of ACFG was significantly decreased. The epidermal thickness of CG was significantly increased compared to that of normal group, while that of ACFG was significantly decreased compared to that of CG These results indicated that the Acanthopanacis cortex water extract administration improved lipid preoxidation, antioxidative activity, serum Ig E level and epidermal thickness in rats with ACD.

본 실험은 오가피열수추출물이 알레르기성 접촉 피부염을 유발한 흰쥐의 체내 지질 조성, 항산화활성, serum Ig E 수준과 표피회복능에 미치는 영향을 조사하기 위해 행해진 것으로 1-chloro-2,4-dinitrochlorobenzene(DNCB: 2.5% DNCB 1 mL로 감작, 4일 경과 후 1% ONCB 1 mL로 유발) 처리로 알레르기성 접촉 피부염이 유발된 Sprague-Dawley 수컷 흰쥐에 오가피열수추출물 (100 mg/체중 kg/day)을 경구투여 한 다음 혈장과 간의 지질조성과 항산화활성, 혈중 Ig E 농도, 표피두께변화를 관찰하여 다음과 같은 결과를 얻었다. 혈장의 total cholesterol과 LDL-cholesterol은 정상군에 비해 ACFG군에서 유의적으로 감소하여(p<0.05) 오가피 열수추출물이 지질개선에 효과적인 것으로 나타났다. 혈장과 간의 지질과산화물 함량은 정상군에 비해 CG군과 ACFG군에서 지질과산화물 함량이 유의하게 높았으며(p<0.05), ACFG군이 CG군에 비해 과산화물 함량이 감소하는 경향을 보였다. 간의 SOD효소활성은 CG군에서 정상군에 비해 유의하게 증가하였으며(p<0.05), ACFG군에서 정상군의 수준으로 유의하게 감소하였다(p<0.05). Serum 중 Ig E 수준은 정상군에 비해 CG군에서 유의하게 증가하였으며(p<0.05), ACFG군에서 CG군에 비해 정상군의 수준으로 유의하게 감소하였다(p<0.05). 표피두께는 정상군에 비해 CG군에서 표피두께가 유의적으로 증가하였으며(p< 0.05), ACFG 군에서 CG군에 비해 유의하게 감소하였다(p<0.05). 혈장과 간의 지질과산화물 함량과 표피두께의 상관계수가 각각 r=0.644(p<0.01), r=0.516(p<0.05) 로 비교적 높은 정의 상관관계를 보였다. 이상의 결과로 미루어 볼 때 정상 흰쥐에 비하여 DNCB에 의해 유도된 접촉성 피부염을 가진 흰쥐의 혈장과 간의 지질과산화물 생성이 증가되며 이로 인해 표피의 두께가 증가하고, SOD 활성이 증가하는 것으로 나타났다. 오가피열수추출물의 섭취로 알레르기성 접촉피부염으로 유발된 흰쥐의 체내 지질과산화물 생성 억제, Ig E 농도감소, 표피두께 감소되는 효과가 나타났다.

Keywords

References

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