Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Study of Reaction Products and Color Changes in Glutamine-Glucose Model System during Heating

Glutamine-Glucose Model System에서 가열조건별 반응생성물과 색의 변화

  • 이영근 (부산대학교 생명응용과학부)
  • Published : 2006.08.30
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Abstract

The product formation and changes in color of glucose/glutamine model system were investigated in relation to heating temperature and time. The mixtures of glucose and glutamine in equal molar ratio were heated at 125, 150 and $175^{\circ}C$ for 10, 20 and 30 minute, respectively. Acetic acid, butanoic acid, 2-butenoic acid, di-(2-cthylhexyl)phthalate, 2,3-dihydro-3,5-dihydroxy-6-methly-4H-pyran-4-one and 5-hydroxymethylfurfural were identified as a major compounds, and 1,3-dimethylbenzene, 2-ethylhexanol, furfural, 5-methylfurfural, 2-pyrrolidinone, and 2,6-di(t-butyl)-4-hydroxy-4-methyl-2.5-cyclohexadien-1-one as 6 minor compounds by using GC/MS. The contents of acetic acid, 2-ehylhexanol and 2-pyrrolidinone increased with increased heating temperature and time, whereas the formation of the other 9 compounds increased up th heating conditions of $150^{\circ}C$ for 10 or 20 min or $175^{\circ}C$ for 10 min, and decreased dramatically with heating above those conditions. Color parameter $L^*$ decreased with increasing heating conition, resulting in dark brown color in final products. Changes of redness parameter $a^*$ and yellowness $b^*$ showed similar to those of the contents of 9 compounds mentioned as above.

Glucose-glutamine 모델반응에서 생성물의 종류와 갈변에 의한 색의 변화를 조사하기 위하여, 가열조건을 125, 150 및 $175^{\circ}C$에서 각각 10, 20 및 30분간 가열하고 반응물의 색을 Hunter colorimeter로 측정한 후, ethyl acetate로 추출하여 GC/MS로 생성물을 분석하였다. GC/MS 분석에서 acetic acid, butanoic acid, 2-butenoic acid, di-(2-ethylhexyl) phthalate, 2,3-dihydro-3.5-dihydroxy-6-methyl-4H-pyran-4-one 및 5-hydroxymethylfurfural의 6종의 다량 생성물과 1,3-dimethylbenzene, 2-ethylhexanol, fufural, 5-methylfurfural, 2-pyrrolidinone 그리고 2,6-di(t-butly)-4-hydroxy-4-methyl-2,5-cyclohexadien-1-one 6종의 미량 생성물이 확인되었다. 최대 생성물인 acetic acid와 미량 생성물인 2-ethylhexanol 및 2-pyrrolidinone은 온도 및 가열시간의 경과에 따라 전반적으로 증가하였으나, 그 외 9종의 화합물을은 $125^{\circ}C$부터 생성량이 증가하지만 $150^{\circ}C$ 또는 $175^{\circ}C$를 정점으로 그 이상 온도, 또는 가열시간을 증가할수록 생성량은 감소하였다. 반응생성물이 Hunter $L^*$값은 온도 및 시간의 증가에 따라 지속적으로 감소하여 암색이 반응의 최종산물에 의한 색으로 나타났으며, $a^*$ 및 $b^*$은 $125^{\circ}C$에서 낮고 $150^{\circ}C$ 또는 $175^{\circ}C$, 10분에서 최고점을 나타낸 후 온도 및 시간의 증가에 따라 감소하여 적색과 황색은 반응 중간생성물에 의한 발현이며 반응후기에는 고분자의 화합물에 고착되어 암색을 발현하는 것으로 추정되었으며, 가열조건의 변화에 따른 $a^*$ 및 $b^*$값의 변화는 앞서 언급한 9종 화합물들의 생성 변화와 유사하였다.

Keywords

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