Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Preparation and Functional Properties of Enzymatic Oyster Hydrolysates

굴 효소 가수분해물의 제조 및 기능특성

  • Chung, In-Kwon (Division of Marine Life Science, Institute of Marine Industry, Gyeongsang national University) ;
  • Kim, Hye-Suk (Division of Marine Life Science, Institute of Marine Industry, Gyeongsang national University) ;
  • Kang, Kyung-Tae (Division of Marine Life Science, Institute of Marine Industry, Gyeongsang national University) ;
  • Choi, Yeung-Joon (Division of Marine Life Science, Institute of Marine Industry, Gyeongsang national University) ;
  • Choi, Jong-Duck (Division of Marine Life Science, Institute of Marine Industry, Gyeongsang national University) ;
  • Kim, Jin-Soo (Division of Marine Life Science, Institute of Marine Industry, Gyeongsang national University) ;
  • Heu, Min-Soo (Division of Marine Life Science, Institute of Marine Industry, Gyeongsang national University)
  • 정인권 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 김혜숙 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 강경태 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 최영준 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 최종덕 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 김진수 (경상대학교 해양생명과학부/해양산업연구소) ;
  • 허민수 (경상대학교 해양생명과학부/해양산업연구소)
  • Published : 2006.08.30
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Abstract

The study was carried out to prepare oyster hydrolysates by using Alcalase, Flavourzyme, Neutrase, Protamex, pepsin and trypsin, and to investigate its functional properties. The ACE inhibitory activity and antioxidant activity of enzymatic oyster hydrolysates did not increase with hydrolysis time. Among enzymatic oyster hydrolysates, oyster hydrolysates incubated with Protamex for 1 hr (OHP) showed the most excellent ACE inhibitory activity and antioxidant activity, and their $IC_{50}$ values were 1.16 mg/mL and 1.49 mg/mL, respectively. However, all enzymatic oyster hydrolysates were not detected in antimicrobial activity.

양식 굴을 효율적으로 이용할 목적으로 6가지의 상업적 효소(Alcalase, Flavourzyme, Neutrase, Protamex, pepsin, trypsin)를 이용하여 굴 효소 가수분해물을 제조하고, 그 특성에 대하여 살펴보았다. 상업적 효소 굴 가수분해물의 항산화능 및 ACE 저해능은 모두 Protamex로 1시간동안 가수분해시킨 것이 가장 우수하였고, 이 때 이들의 $IC_{50}$값은 각각 1.16 mg/mL 및 14 mg/mL이었다. 하지만 항균성은 모든 효소 가수분해물에서 인정되지 않았다. 또한, ACE 저해능 및 항산화능은 가수분해 시간에 따른 의존성이 인정되지 않았다. Protamex로 1시간동안 처리한 굴 가수분해물의 경우 가수분해 처리하지 않은 대조구에 비하여 $29{\sim}66\;kDa$ 획분과 6.5 kDa 부근의 획분이 모두 감소하여 저분자화 하는 경향을 나타내었다.

Keywords

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