The Antioxidative and Nitrite Scavenging Ability of Solvent Extracts from Wild Grape (Vitis Coignetiea) Skin

머루 과피 용매추출물의 항산화성 및 아질산염 소거작용

This study was worked out to investigate antioxidant activity of solvent extracts from wild grape skin by measuring electron donating ability (EDA), reducing power, superoxide dismutase (SOD)-like activity, thiobarbituric acid reactive substances (TBARS) and nitrite scavenging ability. Total phenolic compound and flavonoids contents were the highest in ethyl acetate extract, $54.4{\pm}1.18\;mg/100\;g$ and $645.1{\pm}5.05\;mg/100\;g$, respectively. The EDA and reducing power of solvent extracts from wild grape skin were proportionally increased with concentration and ethyl acetate extract $(79.2{\pm}0.06%)$ showed the stronger than BHT $(74.1{\pm}0.15%)$ at concentration of $100\;{\mu}g/mL$, especially. SOD-like ability of ethyl acetate $(25.1{\pm}0.41%)$ and butanol $(20.2{\pm}0.13%)$ extracts were stronger than other extracts at concentration of $100\;{\mu}g/mL$. TBARS of ethyl acetate extract was higher than ascorbic acid. Nitrite scavenging ability of solvent extracts from wild grape skin (pH 2.5, $1000\;{\mu}g/mL$) were in order of ethyl acetate $(90.5{\pm}0.75%)$>butanol $(65.9{\pm}2.16%)$>hexane $(58.1{\pm}1.74%)$>chloroform $(55.4{\pm}1.02%)$>water $(40.9{\pm}0.35%)$. Antioxidant activity of solvent extracts from wild grape skin was the highest in ethyl acetate extract from the results of our experiments.

머루 과피 용매추출물의 전자공여 작용, 환원력, SOD 유사활성, TBARS 및 아질산염 소거작용을 측정하여 항산화 기능을 규명하고자 하였다. 총 페놀과 플라보노이드 함량이 가장 높은 ethyl acetate 추출물의 경우 전자공여 작용도 $100\;{\mu}g/mL$ 농도에서 $79.2{\pm}0.06%$로 BHT$(74.1{\pm}0.15%)$보다 더 활성이 높았고, 추출물의 농도에 비례하여 전자공여 작용과 환원력은 유의적으로 증가하였다. SOD 유사활성은 $100\;{\mu}g/mL$ 농도에서 ethyl acetate와 butanol 추출물에서 각각 $25.1{\pm}0.41%$와 $20.2{\pm}0.13%$로 다른 추출물에 비해 높은 활성을 보였다. $FeCl_2$와 $CuSO_4$에 대한 항산화 활성도 추출물의 농도가 높아짐에 따라 그 활성이 증가하는 경향이었는데, ethyl acetate 추출물은 ascorbic acid보다 항산화 활성이 높았다. 머루 과피 용매추출물의 아질산염 소거작용은 pH 2.5에서 ethyl acetate$(90.5{\pm}0.75%)$>butanol$(65.9{\pm}2.16%)$>hexane$(58.1{\pm}1.74%)$>chloroform$(55.4{\pm}1.02%)$>water$(40.9{\pm}0.35%)$ 추출물의 순으로 소거작용이 높았다. 이상의 결과를 종합하여 볼 때 머루 과피의 항산화 활성은 ethyl acetate 추출물에서 가장 우수하였다.