Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant and Antimicrobial Activities of Extracts from Sarcodon aspratus

능이버섯(Sarcodon aspratus) 추출물의 항산화성과 항균성

  • 윤경영 (영남대학교 식품영양학과) ;
  • 이숙희 (영남대학교 식품영양학과) ;
  • 신승렬 (대구한의대학교 한방식품조리영양학부)
  • Published : 2006.08.30
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Abstract

The antioxidative and antimicrobial activities were determined on the mushroom (Sarcodon aspratus) extracts in order to find out new food functional components. The antioxidative activities of water and ethanol extracts from the Sarcodon aspratus were measured by peroxide values (POV), electron-donating ability (EDA) using 1,1-diphenyl-2-picryl hydroxyl (DPPH), nitrite-scavenging ability and superoxide dismutase-like activity (SODA) by pyrogallol. The antioxidative activity of the ethanol extract measured by POV was higher than those of the water extract, BHT, and ${\alpha}-tocopherol$. The EDA of the water extract and ethanol extract using DPPH showed the highest values of 76.94% and 73.06%, respectively. The nitrite-scavenging abilities (pH 1.2, 1,000 ppm) of the water and ethanol extracts were 72.61% and 62.69%, respectively, and the nitrite-scavenging ability of the water extract was higher than that of the ethanol extract in all pH values. The SODA of the ethanol extract was higher than that of the water extract. The Sarcodon aspratus extracts had antimicrobial effects on Listeria monocytogenes and Staphylococcus aureus.

본 연구는 식품, 약용, 유전자원 등 풍부한 가치를 가지고 있는 능이버섯에 대하여 기능성 식품의 개발타당성을 입증하기 위하여 능이버섯을 열수와 에탄올로 추출하고 생리활성효과를 측정하였다. 과산화물가(POV)는 항산화제로 사용되는 ${\alpha}-tocopherol$과 BHT보다 능이버섯 에탄올추출물이 높은 항산화 작용을 나타내었다. 능이버섯 추출물의 전자공여능을 측정한 결과는 능이버섯 추출물의 농도에 비례하여 전자공여능이 증가하였으며, 열수추출물이 에탄올추출물보다 전자공여능이 높았다. 능이버섯 추출물의 pH에 따른 아질산염 분해능과 농도에 따른 아질산염 분해능을 조사한 결과 추출물의 농도가 증가할수록 아질산염 분해능이 증가하였고, 열수추출물이 에탄올추출물보다 분해작용이 좀 더 높게 나타났다. 능이버섯의 SOD 유사활성은 에탄올추출물이 열수추출물보다 높게 나타내었다. 추출물에 대한 항균활성은 6종류의 식중독세균 Bacillus subtilis KCTC 1659, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 13565, Gram 음성균인 Escherichia coli O157:H7 ATCC 43895, Salmonella Typhimurium ATCC 7988, Shigella sonnei KCTC 2009을 실험균주로 사용하였다. 능이버섯의 항균활성은 열수추출물과 에탄올추출물 모두 3%, 5% 농도에서 양성균인 L. monocytogenes와 S. aureus에 대하여 항균활성을 나타내었다.

Keywords

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