Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Immuno-Regulatory Activities of an Isoflavone Glycoside, 4', $6-Dimethoxylsoflavone-7-O-{\beta}-D-Glucopyranoside$ and the Crude Extract Isolated from Amorpha fruticosa LINNE

  • Kim, Jung-Hwa (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Cheol-Hee (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kwon, Min-Cheol (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Hyou-Sung (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Kang-Yoon (UNION) ;
  • Lee, Hyun-Jung (Division of Wood Chemistry, Korea Forest Research Institute) ;
  • Kang, Ha-Young (Division of Wood Chemistry, Korea Forest Research Institute) ;
  • Lee, Hak-Ju (Division of Wood Chemistry, Korea Forest Research Institute) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University)
  • Published : 2006.04.01
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Abstract

The methanolic (MeOH) extract of A. fruticosa bark, which showed immune-regulatory activities, was separated to purify an active compared by means of a multi-stage column chromatography. This resulted in the isolation and characterization of an isoflavone glycoside named 4', $6-Dimethoxyisoflavone-7-O-{\beta}-D-glucopyranoside$. Immuno-regulatory activities of the crude extract of Amorpha fruticosa LINNE bark were compared with that of an isoflavone glycoside (4', $6-dimethoxyisoflavone-7-O-{\beta}-D-glucopyranoside$). The crude methanolic extract of A. fruticosa and purified single compound showed 16% of relatively low cytotoxicity at a maximum concentration of 1.0 g/L in cultivated normal human lung cell line (HEL299). Cell growth of human T cells was increased up to 15%, 0.5 g/L of the crude extract added group. This was higher than a single compound added one. On the other hand, specific production rates of IL-6 and $TNF-{\alpha}$ from T cell were higher in the purified compound treat group ($0.82{\times}10^{-4}\;pg/cell$ and $1.08{\times}10^{-4}\;pg/cell$, respectively), compared to 0.5 g/L of the crude extract added group ($0.65{\times}10^{-4}\;pg/cell$ and $0.84{\times}10^{-4}\;pg/cell$, respectively). In addition, the growth of NK-92MI cells incubated with the crude extract was higher up to 56% over the cells grown with a single compound (0.5 g/L). In overall, the crude extract showed relatively higher immuno-regulatory activities compared with a single compound, probably due to the synergic effect given by other substances existed in the crude extract. Even though the siolated compound stimulated higher secretion of cytokines from human T cells.

Keywords

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