Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Plant Regeneration Via Organogenesis on Petiole of Centella asiatica (L.) Urban

  • Choi, Kyung-Mi (Dept. of Biology and Institute of Plant Resources, Chonnam Nat'l. Univ.) ;
  • Hwang, Sung-Jin (Dept. of Oriental Medicine Materials, Dongshin Univ.) ;
  • Chung, Sang-Jin (Dep. of Genetic Engineering, Chongju University) ;
  • Hwang, Baik (Dept. of Biology and Institute of Plant Resources, Chonnam Nat'l. Univ.)
  • Published : 2006.04.01
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Abstract

An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA,2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA $17.13\;{\mu}M$ and BA $8.9\;{\mu}M$. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil.

Keywords

References

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