Comparison of Valerenic Acids and Valepotriates Production According to the Culture Conditions for Cultured Roots of Valeriana fauriei var. dasycarpa Hara

  • Li, Mei-Yang (Department of Biology & Institute of Plant Resources, Chonnam National University) ;
  • Ahn, Jun-Cheul (Department of Life Sciences, Seonam University) ;
  • Kim, Kwang-Soo (Department of Biology & Institute of Plant Resources, Chonnam National University) ;
  • Kim, Ok-Tae (Department of Biology & Institute of Plant Resources, Chonnam National University) ;
  • Park, Yoon-Jung (Department of Biology & Institute of Plant Resources, Chonnam National University) ;
  • Hwang, Baik (Department of Biology & Institute of Plant Resources, Chonnam National University)
  • Published : 2006.04.01

Abstract

We established a practical method for rapid and large-scale production of Valeriana fauriei var. dasycarpa Hara roots by bioreactor culture and confirmed valerenic acids and valepotriates production. We also compared valerenic acids and valepotriates production patterns according to various media conditions. Among the media tested, B5 medium gave the maximum biomass production of 101 g fresh weight, which was a 5.03-fold multiplication rate obtained 4 weeks after inoculation of 20 g of fresh weight. The best production of total valerenic acids $(7.86\;mg/l)$ and valepotriates $(8.96\;mg/l)$ was B5 medium.

Keywords

References

  1. Banerjee S, Rahman L, Uniyal GC Ahuja PS (1998) Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC. Plant Sci. 131:203-208 https://doi.org/10.1016/S0168-9452(97)00237-9
  2. Bicchi C, Binello A, Rubiolo P (2000) Packed column SFC/UV versus HPLC/UV analysis of valerenic acids and valepotriates in extract of Valeriana of licinalis L. Phytochemical Anal. 11:179-183 https://doi.org/10.1002/(SICI)1099-1565(200005/06)11:3<179::AID-PCA515>3.0.CO;2-5
  3. Blumenthal M (2001) Herb sales down 15% in mainstram market. Herbal Gram 59:69
  4. Bos R, Woerdenbag HJ, Hendricks H, Zwaving JH, De Smet PAGM, Tittle G, Wikstrom HV, Scheffer JJC (1996) Analytical aspects of phytotherapeutic Valerian preparations. Phytochemical Anal. 7:143-151 https://doi.org/10.1002/(SICI)1099-1565(199605)7:3<143::AID-PCA284>3.0.CO;2-1
  5. Bos R, Woerdenbag HJ, Van Putten FMS, Hendricks H, Scheffer JJC (1998) Seasonal variation of the essential oil, valerenic acid and derivatives, and va1epotriates in Valeriana officinalisroots and rhizomes, and the selection of plant suitable for phytomedicines. Planta Med. 64:143-147 https://doi.org/10.1055/s-2006-957392
  6. Bos R, Woerdenbag HJ, Pras N (2002) Determination of valepotriates. Chromatography A 967:131-146 https://doi.org/10.1016/S0021-9673(02)00036-5
  7. Caetano LC, Charlwood BV, Gahan PBBos (1999) The localisation and acumulatition of valepotriates in hairy roots of Valerianella disco idea (L.) Loisel. Phytochem. Ana. 10:181-186 https://doi.org/10.1002/(SICI)1099-1565(199907/08)10:4<181::AID-PCA442>3.0.CO;2-4
  8. Gamborg OL, Miller RA, Ojimaki K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151-158 https://doi.org/10.1016/0014-4827(68)90403-5
  9. Gao XQ, Bjork L (2000) Valerenic acid derivatives and valepotriates among individuals, varieties and species of Valeriana. Fitoterapia 71:19-24 https://doi.org/10.1016/S0367-326X(99)00094-5
  10. Granicher F, Christen P, Kapetanidis I (1992) High-yield production of valepotriates by hairy root cultures of Valeriana ofjiconalis L. var. sambucifolia Mikan. Plant Cell Rep. 11 :339-342
  11. Granicher F, Christen P, Kapetanidis I (1995) Production of valepotriates by hairy root cultures of Centranthus ruber DC. Plant Cell Rep. 14:294-298
  12. Houghton PJ (1988) The biological activity of valerian and related plants. Ethnopharmacol. 22:121-142 https://doi.org/10.1016/0378-8741(88)90123-7
  13. Kim SK, Kim KS, Kim YH, Lee DC, Ahn DJ, Kim YH (2003) Isolation and identification of biologically active components from Korean valerian roots. Korean J. Society of Tobacco Sci. 25(1):80-86
  14. Kittipongpatana N, Davis DL, Porter JR (2002) Methyl jasmonate increases the production of valepotriates by transformed root cultures of Valerianella locusta. Plant Cell, Tissue and Organ Cul. 71:65-75 https://doi.org/10.1023/A:1016517019327
  15. Letchamo W, Ward W, Heard B, Heard D (2004) Essential oil of Valeriana officinalis L. cultivars and their antimicrobial activity as influenced by harvesting time under commercial organic cultivation. Agriculture and Food Chem. 52:39153919 https://doi.org/10.1021/jf0353990
  16. Lloyd GB, Mc Cown BH (1980) Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tip culture. Proceed. Internl. Plant Propagation 30:421-427
  17. Murashige E, Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiol. Plant. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Park SH (1972) A study of the genus Valeriana in Korea. Plan Taxonom. Soc. Korea 3:25-28
  19. Schenk RD, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199-204 https://doi.org/10.1139/b72-026
  20. Wills RBH, Shohet D (2003) Production of high quality Australian valerian products. A report for the rural industries research and development corporation p. 1-39