Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Effects of Several Pre-treatments on Seed Germination or Sophora japonica L.

회화나무 종자발아에 미치는 전처리의 효과

  • Tak, Woo-Sik (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Tae-Su (Forest Seed Research Center, Korea Forest Research Institute) ;
  • Choi, Chung-Ho (Department of Forest Genetic Resources, Korea Forest Research Institute)
  • 탁우식 (국립산림과학원 산림유전자원부) ;
  • 김태수 (국립산림과학원 산림종자연구소) ;
  • 최충호 (국립산림과학원 산림유전자원부)
  • Published : 2006.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

This experiment was conducted to study the effects of sulphuric acid, cutting, cold stratification and hot water on the germination of Sophora japonica seeds, which have difficulty to germinate because they have hard and thick seed coats. The seeds were immersed in 30, 60 and 90% sulphuric acid for 30 minutes as seed scarifications, and cut 1/8, 2/8 and 3/8 of the opposite parts of radicles. As cold stratification the seeds were wrapped in wet towel, and then stored in plastic bags in a refrigerator $(4^{\circ}C)$ for 3, 5 and 10 days. The seeds were immersed in hot water $(90{\sim}95^{\circ}C)$ for 2,5 and 10 minutes. Pretreated seeds represented different germination properties, respectively. After sulphuric acid treatment, the seeds showed an increase in germination (G) and germination index (GI). Compared to the other treatments, 90% sulphuric acid showed the highest G (31.7%) and GI (6.2). The G and GI of cut seeds decreased with the increase of seed cutting lengths. And G and GI of cold stratificated seeds were not significantly different among the days treated (p=0.258). Two minutes treatment of hot water showed lower G and Gl than control, and the seeds were not germinated in 5 and 10 minutes treatments of hot water. At the result of relative growth rate and T/R ratio of seedlings from pretreated seeds, the seedlings from seeds in 90% sulphuric acid treatment represented the highest relative growth rate and T/R ratio.

본 연구는 경립종자로서 종피가 단단하고 두꺼워 발아시 어려움이 있는 회화나무 종자를 여러 가지 전처리를 통해 발아율 및 발아지수를 향상시키고자 실시하였다. 종피 파상처리로서 황산 30, 60, 90% 용액에 30분간 침지하였고, 종자의 유근 반대편 부위를 1/8, 2/8, 3/8씩 절단하였다. 또한 종자를 젖은 수건에 싸서 밀봉 후 $4^{\circ}C$ 저온고에 각각 3, 5, 10일간 보관하였으며, $90{\sim}95^{\circ}C$ 뜨거운 물에 각각 2, 5, 10분간 침지하였다. 전처리된 종자는 각각 다른 발아특성을 나타내었다. 황산 처리된 종자는 발아율 및 발아지수에서 무처리구 보다 높게 나타났다. 특히, 황산 90%처리에서 가장 높은 효과를 보여주었다. 종자 절단 처리에서는 절단 길이가 늘어날수록 발아율 및 발아지수가 감소하였으며 종자의 냉층적 처리에서는 차이를 보이지 않았다(p=0.258). 열탕 처리시 2분 침지 종자는 무처리 종자보다 발아율 및 발아지수가 낮게 나타났으며 5, 10분 침지 종자는 발아하지 않았다. 전처리가 종자 발아뿐만 아니라 유묘활력에도 영향을 미치는지 알아보고자 전처리 종자로부터 생장한 유묘를 대상으로 상대생장율 및 T/R율을 조사한 결과 황산 90% 처리구의 유묘에서 가장 뛰어난 수고 및 근원경의 상대생장율과 T/R율을 관찰 할수 있었다. 위의 결과로서 황산 90% 용액에 30분간 침지처리를 했을 때 회화나무 종자의 발아율, 발아지수를 비롯한 유묘의 상대생장율 및 T/R율이 높게 나타남을 알 수 있었다.

Keywords

References

  1. Beadle, C.L. 1993. Growth analysis. Photosynthesis and Production in a Changing Environment. A field and Laboratory Manual. D. O. Hall. J. M. O. Scurlock. H. R. Bolhar- Nordenkampf. R. C. Leegood and S. P. Long (Eds.). Chapman ＆ Hall. London. pp. 36-46
  2. Bevilacqua, L.R., F. Fossati and G. Dondero. 1987. Callose in the impermeable seed coat of Sesbania punicea. Ann. Bot. 59: 335-341 https://doi.org/10.1093/oxfordjournals.aob.a087322
  3. Demel, T. 1996. The effect of different pre-sowing seed treatments, temperature and light on the germination of five Senna species from Ethiopia. New For. 11: 155-171 https://doi.org/10.1007/BF00033411
  4. Demel, T and T. Mulualem. 1996. The effect of pre-sowing seed treatments, temperature and light on the germination of Tamarindus indica L., a multipurpose tree. J. Trop. For. 12: 73-79
  5. Drr, C. and J. Boiffin. 1995. Sugarbeet (Beta vulgaris L.) seedling growth from germination ti first leaf stage. The journal of Agri. Sci. 124: 427-435 https://doi.org/10.1017/S002185960007338X
  6. Goo, G.H., K.S. Youn and J.S. Choi. 1993. Seed germination improvement by pon-pon treatment and asexual multiplication by cutting in Zanthoxylum piperitum. Kor. J. For. Soc. 82: 227-234
  7. Huang, Z.Y. and Y. Gutterman. 2000. Comparison of germination strategies of Artemisia ordosica with its two congeners from deserts of China and Israel. Acta Bot. Sinica 42: 71-80
  8. Khan, M.L., P. Bhuyan, U. Shankar and N.P. Todaria. 1999. Seed germination and seedling fitness in Mesua ferra L. in relation to fruit size and seed number per fruit. Acta Oecol. 20: 599-606 https://doi.org/10.1016/S1146-609X(99)00101-0
  9. Lee, J.K., S.H. Kim and S.J. Lee. 1992. Promotion germination by physicochemical in Liriope platyphylla. Res. Rept. RDA(U&I) 34: 125-129
  10. Manning, J.C. and J. van Staden. 1987. The role of the lens in seed inhibition and seeding vigor of Sesbania punicea (Cav.) Benth. (Leguminosae : Papilionnoideae). Ann. Bot. 59: 705-713
  11. Paul, K.B., C.S. Patel and P.K. Biswas. 1973. Changes in endogenous growth regulator in loblolly pine seeds during the proess of stratification and germination. Physical. Plant. 28: 530-534 https://doi.org/10.1111/j.1399-3054.1973.tb08601.x
  12. Ren, J. and Tao, L. 2004. Effects of different pre-sowing seed treatments on germination of 10 Calligonum species. For. Ecol. and Manag. 195: 291-300 https://doi.org/10.1016/j.foreco.2004.01.046
  13. Schutz, W. and G. Rave. 1999. The effect of cold stratification and light on the seed germination of temperature sedges (Carex) from various habitats and implications for regenerative strategies. Plant Ecol. 144: 215-230 https://doi.org/10.1023/A:1009892004730
  14. Scott, S.J., R.A. Jones and W.A. Williams. 1984. Review of data analysis methods for seed germination. Crop Sci. 24: 1160-1162
  15. Scowcroft, P.G. 1978. Germination of Sophora chrysophylla increased by presowing treatment. Forest Service, UDSA. Berkeley. CA
  16. Scowcroft, P.G. 1981. Regeneration of mamane: effects of seed coat treatment and sowing depth. For. Sci. 27: 771-779
  17. Tamet, V., J. Boffin, C. Durr and N. Souty. 1996. Emergence and early growth of an epigeal seedling (Daucus carota L.) : influence of soil temperature, sowing depth, soil crusting and seed weight. Soil & Tillage Res. 40: 25-38 https://doi.org/10.1016/S0167-1987(96)01044-6
  18. Tao, L. 2000. Genetic diversity and systematical taxonomy of genus Calligonum L. PhD Thesis. Environmental and Engineering Institute of Cold and Arid Regions. The chinese Academy of Sciences. PR China (in Chinese with English abstract)
  19. Tao, L., J. Ren and X.M. Liu. 2000. Study on the water-absorbing model of two Calligonum species seeds. J. Arid Land Resour. Environ. 14: 89-91 (in Chinese with English abstract)
  20. Tomer, R.P.S. and K. Slingh. 1993. Hard seed studies in rice bean (Vigna umbellata). Seed Sci. & Technol. 21: 679-683
  21. Virginia C., C. Julia and V. Carlos. 1996. Seed germination of woody legumes from deciduous tropical forest of southern Mexico. For. Ecol. and Manag. 82: 171-184 https://doi.org/10.1016/0378-1127(95)03671-7
  22. Yang, J., J. Lovett-Doust and L. Lovett-Doust. 1999. Seed germination patterns in green dragon (Arisaema dracontium, Araceae). Am. J. Bot. 86: 1160-1167 https://doi.org/10.2307/2656980
  23. Yu, Z. and L.H. Wang. 1998. Causes of seed dormancy of three species of Calligonum. J. NW For. College 13: 9-13 (in Chinese with English abstract)
  24. 김인재,김민자,남상영,박재호,이철희,김홍식.2003.초피나무종자의 전처리가 포장에서의 출아에 미치는 영향. 한국자원식물학회지 16: 141-146
  25. 이봉수, 윤종규, 이명보. 1984. 건묘 육성을 위한 유묘규격에 관한 연구. 임연연보. 31: 20-30
  26. 임록재. 1999. 조선약용식물지1. 한국문화사. pp. 209-210
  27. 임업연구원. 1987. 한국수목도감. 국립산림과학원홈페이지산림관련 정보. http://www.kfri.go.kr
  28. 조혜경, 홍성각, 김종진. 2001. 상대광도차이에따른구상나무유묘의 생장과 물질생산에 관한 연구. 임산에너지 20: 58-68
  29. 한심희,김찬수,장석성,이현주,탁우식.2004. 저장기간에 따른 3개 수종의 종자 및 발아특성 변화. 한국농림기상학회지 6: 183-189