THE EFFECT OF PULSED ELECTROMAGNETIC FIELDS ON ${\beta}$-TCP GRAFT IN RABBIT CRANIAL BONE DEFECT

가토 두개골 결손부에 이식된 ${\beta}$-TCP의 골치유과정에서 맥동전자기장의 영향에 관한 연구

  • Kim, Sang-Woo (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Hwang, Kyung-Gyun (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Lim, Byung-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Park, Chang-Joo (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Chung, Il-Hyuk (Department of Dentistry/Oral and Maxillofacial Surgery, Seoul National University Boramae Hospital) ;
  • Paik, Seung-Sam (Department of Pathology, College of Medicine, Hanyang University) ;
  • Shim, Kwang-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University)
  • 김상우 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 황경균 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 임병섭 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 박창주 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 정일혁 (서울대학교 보라매 병원 구강악안면외과) ;
  • 백승삼 (한양대학교 의과대학 병리학교실) ;
  • 심광섭 (한양대학교 의과대학 치과학교실 구강악안면외과)
  • Published : 2006.08.31

Abstract

The purpose of this research was to investigate whether pulsed electromagnetic field (PEMF) stimulation applied to the rabbit cranial defects grafted with ${\beta}$-tricalcium phosphate (${\beta}$-TCP) could affect the new bone formation. With 16 New Zealand white rabbits under the same condition, bilateral calvarial bone defects were formed around the sagittal suture line. The defect on the left side was grafted with ${\beta}$-TCP, while on the right side was grafted by harvested autogenous bone. PEMF was applied to 8 rabbits for 8 hours per day. The bony specimen were divided into 3 groups, the group 1 was autogenous bone grafted specimen, the group 2 was ${\beta}$-TCP grafted with PEMF, and the group 3 was ${\beta}$-TCP grafted without PEMF. We investigated the bone regeneration & growth factor expression at 2, 4, 6, and 8 weeks. As a result, BMP 2 was expressed in the group 1 from 2 weeks, the group 2 from 4 weeks, and the group 3 from 6 weeks. BMP 4 was expressed in the group 1 from 2 weeks, in the group 2 and the group 3 from 4 weeks. 4. There was no significant difference in expression pattern of BMP 7, PDGF, VEGF, and TGF-${\beta}$1 during grafted bone regeneration in group 1, 2, and 3. According to our results, PEMF stimulation could be effective on the new bome formation in animal study, and have a feasibility of clinical use.

References

  1. Laurencin CT: Bone Graft Substitutes. ASTM International 2003;180-187
  2. Merten HA, Wiltfang J, Grohmann U et al: Intraindividual Comparative animal Study of ${\alpha}$-and ${\beta}$-Tricalcium Phosphate Degradation in Conjunction with Simultaneous Insertion of Dental Implants. J Craniofac Surg 2001;12:59-68 https://doi.org/10.1097/00001665-200101000-00010
  3. Szabo G, Suba Z, Hrabak K et al: Autogenous Bone Versus ${\beta}$- Tricalcium Phosphate Graft Alone for Bilateral Sinus Elevations(2- and 3-Dimensional Computed Tomographic, Histologic, and Histomorphometric Evaluations): Preliminary Results. Int J Oral Maxillofac Implant 2001;16:681-692
  4. Bahn S, Plaster: A Bone substitute. Oral Surg Oral Med Oral Pathol 1966;21:672-681 https://doi.org/10.1016/0030-4220(66)90045-4
  5. Saito M, Shimizu H, Beppu M et al: The role of ${\beta}$-Tricalcium Phosphate in vasculized periosteum. J Orthop Sci 2000;5:275-282 https://doi.org/10.1007/s007760050163
  6. Yasuda I: Piezoelectricity of living bone. J Kyoto Pref Univ Med 1953;53:325
  7. Yasuda I: Mechanical and electrical callus. Ann NY Sci 1974;238:457 https://doi.org/10.1111/j.1749-6632.1974.tb26812.x
  8. Peltier LF: A brief historical note on the use of electricity in the treatment of fracture. Clin Orthop 1981;161:4-7
  9. Braden M, Bairstow AG, Beider A, ritter BG: Electrical and piezoelectrical properties of dental hard tissue. Nature 1966;212:1565- 1566 https://doi.org/10.1038/2121565a0
  10. Cochran GVB, Pawluk RJ, Bassett CAL: Stress generated electrical potentials in the mandible and teeth. Arch Oral Biol 1967;12:917- 920 https://doi.org/10.1016/0003-9969(67)90117-3
  11. Norton LA: Implication of bioelectric growth control in orthodontics and dentistry. Angle Orthod 1975;45:34-42
  12. Vingerling PA, Vanderkuij P, Degroot K, Sillevis PAE: Non-invasive treatment of alveolar wounds. In Electrical properties of bone and cartilage. Graune and Stratton. New York 1979;341-346
  13. Branham GB, Triplett RG, Yeandle S, Vieras F: The effect of electrical current on the healing of mandibular freezed dried bone allograft in dog. J Oral Maxillofac Surg 1985;43:403-407 https://doi.org/10.1016/S0278-2391(85)80047-1
  14. Steiner M, Ramp WK: Electrical stimulation of bone and its implications for endosseous dental implantation. J Oral Implant 1990;16:20- 27
  15. Gerling JA, Sinclair PM, Roa RL: The effect of pulsating electromagnetic fields on condylar growth in guinea pigs. Am J Orthop 1985;87:211-223 https://doi.org/10.1016/0002-9416(85)90042-9
  16. Lu J, Descamps M, Dejou J et al: The biodegradation mechanism of calcium phosphate biomaterials in bone. J Biomed Bater Res 2002;63:408-412 https://doi.org/10.1002/jbm.10259
  17. Ducheyne P, Qiu Q: Bioactive ceramics: the effect of surface reactivity on bone formation and bone cell function. Biomaterials 1999;20:2287-303 https://doi.org/10.1016/S0142-9612(99)00181-7
  18. Wiltfang J, Merten HA, Schlegel KA et al: Degradation characteristics of alpha and beta tri-calcium phosphate in minipigs. J Biomed Mater res 2002;63:115-121 https://doi.org/10.1002/jbm.10084
  19. Spadoro JA: Bioelectrical properties of bone and response of bone to electrical stimuli. In Bone. Vol 3, Boston, CRC Press, 1991;109-141
  20. Norton LA, Hanley KJ, Turkewicz J: Bioelectric pertubations of bone. Angle Orthod 1984;54:73-87
  21. Roden GA, Bourrett LA, Norton LA: DNA sytheses in cartilage cell stimulated by oscillating eletrical field. Science 1978;199:690 https://doi.org/10.1126/science.625660
  22. Happenstall BR: Constant direct current treatment for established non-union of the tibia. Clin Orthop 1983;178:179
  23. Norton LA, Rodan GA, Bourret LA: Epiphyseal cartilage c-AMP changes produced by electrical and mechanical perturbations. Clin Orthop 1977;124:59-68
  24. Bassett CAL: The development and application of pulsed electromagnetic fields(PEMFs) for ununited fractures and arthrodeses. Orthop Clin North Am 1984;15:61-88
  25. Bassett CAL, Pilla AA, and Pawluk RJ: A nonoperative salvage of surgically-resistant pseudoarthrosis and non-union by pulsating electromagnetic fields. Clin Orthop 1977;124:128-143
  26. Hass DW: Pulsating electromagnetic current induction of mandibular condyles in the cat. J Dent Res 1984;63-335
  27. Matsumoto H, Ochi M, Abiko Y, Kaku T, Sakaguchi K: Pulsed electromagnetic fields promote bone formation around dental implants inserted into the femur of rabbits. Clin Oral Implants Res 2000;11:354-60 https://doi.org/10.1034/j.1600-0501.2000.011004354.x
  28. Fini M, Cadossi R, Cane V, Cavani F, Giavaresi G, Krajewski A, Martini L, Aldini NN, Ravaglioli A, Rimondini L, Torricelli P, Giardino R: The effect of pulsed electromagnetic fields on the osteointegration of hydroxyapatite implants in cancellous bone: a morphologic and microstructural in vivo study. J Orthop Res 2002;20:756-63 https://doi.org/10.1016/S0736-0266(01)00158-9
  29. Buzza EP, Shibli JA, Barbeiro RH, Barbosa JR: Effects of electromagnetic field on bone healing around commercially pure titanium surface: histologic mechanical study in rabbits. Implant Dent 2003;12:182-7 https://doi.org/10.1097/01.ID.0000058385.23346.4D
  30. Grissett JD: Biological effects of electric and magnetic fields associated with ELF communications systems, Proceeding of IEEE 1980;68:98-104
  31. Bolander MR: Regulation of fracture repair by growth factors. Proc Soc Exp Biol Med 1992;200:165-170
  32. Kloen P, Di Paola M, Borens O et al: BMP signaling components are expressed in human fracture callus. Bone 2003;33:362-371 https://doi.org/10.1016/S8756-3282(03)00191-1
  33. Stenport VF, Johansson C, Heo SJ et al: Titanium implants and BMP- 7 in bone: an experimental model in the rabbit. J Mater Sci Mater Med 2003;14:247-254 https://doi.org/10.1023/A:1022884724059
  34. Urist MR, Raskin K, Goltz D et al: Endogenous bone morphogenic protien : immunohistochemical localization in repair of a punch hole in the rabbit's ear : Plast Reconstr Surg 1997;99:1382-1389 https://doi.org/10.1097/00006534-199704001-00028
  35. Lee SH, Song SI, Hwang KG et al: The experimental study of the regeneration on ${\beta}$-TCP in rabbit cranial bone. J Kor Oral Maxillofac Surg 2004;30:282-291
  36. Watanabe K, Niimi A, Ueda M: Autogenous bone grafts in the rabbit maxillary sinus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999;88:26-32 https://doi.org/10.1016/S1079-2104(99)70189-7
  37. Street J, Bao M, deGuzman L et al: Vascular endotherial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. PNAS 2002;99:9656-9661