가토 두개골 결손부에 이식된 ${\beta}$-TCP의 골치유과정에서 맥동전자기장의 영향에 관한 연구

  • Kim, Sang-Woo (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Hwang, Kyung-Gyun (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Lim, Byung-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Park, Chang-Joo (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University) ;
  • Chung, Il-Hyuk (Department of Dentistry/Oral and Maxillofacial Surgery, Seoul National University Boramae Hospital) ;
  • Paik, Seung-Sam (Department of Pathology, College of Medicine, Hanyang University) ;
  • Shim, Kwang-Sup (Department of Dentistry/Oral and Maxillofacial Surgery, College of Medicine Hanyang University)
  • 김상우 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 황경균 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 임병섭 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 박창주 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 정일혁 (서울대학교 보라매 병원 구강악안면외과) ;
  • 백승삼 (한양대학교 의과대학 병리학교실) ;
  • 심광섭 (한양대학교 의과대학 치과학교실 구강악안면외과)
  • Published : 2006.08.31


The purpose of this research was to investigate whether pulsed electromagnetic field (PEMF) stimulation applied to the rabbit cranial defects grafted with ${\beta}$-tricalcium phosphate (${\beta}$-TCP) could affect the new bone formation. With 16 New Zealand white rabbits under the same condition, bilateral calvarial bone defects were formed around the sagittal suture line. The defect on the left side was grafted with ${\beta}$-TCP, while on the right side was grafted by harvested autogenous bone. PEMF was applied to 8 rabbits for 8 hours per day. The bony specimen were divided into 3 groups, the group 1 was autogenous bone grafted specimen, the group 2 was ${\beta}$-TCP grafted with PEMF, and the group 3 was ${\beta}$-TCP grafted without PEMF. We investigated the bone regeneration & growth factor expression at 2, 4, 6, and 8 weeks. As a result, BMP 2 was expressed in the group 1 from 2 weeks, the group 2 from 4 weeks, and the group 3 from 6 weeks. BMP 4 was expressed in the group 1 from 2 weeks, in the group 2 and the group 3 from 4 weeks. 4. There was no significant difference in expression pattern of BMP 7, PDGF, VEGF, and TGF-${\beta}$1 during grafted bone regeneration in group 1, 2, and 3. According to our results, PEMF stimulation could be effective on the new bome formation in animal study, and have a feasibility of clinical use.


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