Nutritional Sciences

The Korean Nutrition Society (한국영양학회)
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The Effects of Smoking Cessation and Antioxidant Vitamins on Oxidative Stress

  • Ha, Aewha (Department of Food and Nutrition, Soong Eui Women's College)
  • Published : 2006.11.30
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Abstract

In this study, the effects of smoking cessation and relative antioxidant activities on the oxidative stress were determined by using in vitro method. Thirty healthy smokers who were free of any disease and smoked more than 1 pack per day for the past 10 years participated in this study. For smoking cessation, smokers were asked to wear nicotine patch (21mg nicotine/ patch) everyday for 30 days and then to replace at the same time of the day. Smoking cessation program in conjunction with nicotine patch replacement was also conducted every week, one hour/each session, for 4 weeks. Canthaxanthin, $\beta-carotene$, and $\alpha-tocopherol$ were added into red blood cells at pre and post smoking cessation. As indicators of oxidative stress, hemoglobin degradation, lipid peroxidation, and percent hemolysis were determined at both pre and post smoking cessation. After 30 days of smoking cessation, the subjects gained an average of 5 pounds, varying 2 to 8 pounds, by suggesting that behavioral problems rather than nicotine itself are more important for gaining weight in ex-smokers. The total hemoglobin concentrations in blood were similar in pre and post smoking cessation, but smoking cessation resulted in a decrease in the percentage of methemoglobin from 0.96% to 0.85% Smoking cessation also caused to decease malondialdehyde (MDA) values ($26.7{\pm}7.8$ vs. $23.6{\pm}4.5$ (without oxidation), $179.3{\pm}21$ vs. $161.2{\pm}28$ nmol/ml (with oxidation) (p<0.05)), not percent hemolysis. Various antioxidants with smoking cessation significantly decreased MDA values(p<0.05), in contrast to marginal decrease of MDA in smoking cessation only. Three antioxidants used in this stu study were similarly effective in inhibiting MDA production, but relative effectiveness of canthaxanthin or $\alpha-tocopherol$ was greater than that of $\beta-carotene$ (p<0.05), in case of oxidation induced. The percent hemolysis was greatly decreased when antioxidants were added into the blood of ex-smokers (p<0.05) but no statistical significance in relative effectiveness of antioxidants was observed.

Keywords

References

  1. Bakhru A, Erlinger TP. Smoking cessation and cardiovascular disease risk factors: Results from the third national health and nutrition examination survey. PLoS Medicine 2:528-536, 2005
  2. Willemse BW, Postma DS, Timens W, Hacken NH. The impact of smoking cessation on respiratory symptoms, lung function, airway hyper responsiveness and inflammation. Eur Respir J 23:464-476, 2004 https://doi.org/10.1183/09031936.04.00012704
  3. Salonen JT, Yla-Herttuala S, Yamamoto R. Autoantibody against oxidised LDL and progression of carotid atherosclerosis. Lancet 339:883-887, 1992 https://doi.org/10.1016/0140-6736(92)90926-T
  4. Preston AM. Cigarette smoking-nutritional implications. Prog Food Nutr Sci 15:183-191, 1991
  5. Haliwell B, Gutteridge MC. Free radicals in biology and medicine. pp.345, Clarendon Press, Oxford, 1989
  6. Ludwig PW, Hoidal JR. Alterations in leukocyte oxidative metabolism in cigarette smoker. Am Rev Respir Dis 126:977-982, 1982
  7. Mcgowan SE, Parenti CM, Hoidal JR, Niewoenhner DE. Ascorbic acid content and accumulation by alveolar macrophases from cigarette smokers and nonsmokers. J Lab Clin Med 104:127-131, 1984
  8. Hunninghake GW, Crystal RG. Cigarette smoking and lung destruction: Accumulation of neutrophils in the lung of cigarette smokers. Am Rev Repir Dis 128:833-838, 1982
  9. Dale LC, Schroeder DR, Wolter TD, Croghan IT, Hurt RD, Offord KP. Weight change after smoking cessation using variable doses of transdermal nicotine replacement. J Gen Intern Med 13:9-15, 1998 https://doi.org/10.1046/j.1525-1497.1998.00002.x
  10. Jessen AB, Toubro S, Astrup A. Effect of chewing gum containing nicotine and caffeine on energy expenditure and substrate utilization in men. Am J Clin Nutr 6:1442-1447, 2003
  11. Jay M, Kojima S, Gillespie MN. Nicotine potenciates superoxide anion generation by human neutrophils. Tox Appl Pharm 86:484-487, 1986 https://doi.org/10.1016/0041-008X(86)90376-5
  12. Pilz H, Oguogho A, Chehne F, Lupattelli G, Palumbo B, Sinzinger H. Quitting cigarette smoking results in a fast improvement of in vivo oxidation injury (determined via plasma, serum and urinary isoprostane). Thromb Res 99:209-21, 2000 https://doi.org/10.1016/S0049-3848(00)00223-1
  13. James RW, Leviev I, Righetti A. Smoking is associated with reduced serum paraoxonase activity and concentration in patients with coronary artery disease. Circulation 101:2252-2257, 2000 https://doi.org/10.1161/01.CIR.101.19.2252
  14. Leng GC, Horrobin DF, Fowkes FG, Smith FB, Lowe GD, Donnan PT, Ells K. Plasma essential fatty acids, cigarette smoking, and dietary antioxidants in peripheral arterial disease: A population-based case-control study. Arterioscler Thromb 14:471-478, 1994 https://doi.org/10.1161/01.ATV.14.3.471
  15. Zamora R, Hidalgo FJ, Tappel AL. Comparative antioxidant effectiveness of dietary $\beta$-carotene, vitamin E, selenium and coenzyme Q10 in rat erythrocytes and plasma. J Nutr 121:50-56, 1991 https://doi.org/10.1093/jn/121.1.50
  16. Trotta RJ, Sullivan SG, Stem A. Lipid peroxidation and haemoglobin degradation in red blood cells exposed to t-butyl hydroperoxide. Biochem J 204:405-409, 1982 https://doi.org/10.1042/bj2040405
  17. Harley JD, Mauer AM. Studies on the formation of Heinz Bodies. Blood 16:1722-1728, 1960
  18. Michale HO, Harris JS. The blood pigments: the properties and quantitative determination with special reference to the spectrometric methods. J Lab Clin Med 25:455-463, 1940
  19. Evelyn KA, Malloy HT. Microdetermination of oxyhemoglobin, methemoglobin and sulfhemoglobin in a single sample of blood. J Biol Chem 126:655-659, 1938
  20. Stocks J, Dormandy TL. The autoxidation of human red cell lipids induced by hydrogen peroxide. Bir J Haematol 20:95-100, 1971 https://doi.org/10.1111/j.1365-2141.1971.tb00790.x
  21. Niki E, Komuro E, Takahashi M, Terao K. Oxidative hemolysis of erythrocytes and its inhibition by free radical scavengers. J Biol Chem 263:19799-19803, 1988
  22. Flegal KM, Troiana RP, Pamuk ER, Kuczmarski RJ, Campbell SM. Influence of smoking cessation on the prevalence of overweight in the United States. New Engl J Med 333:1165-1170, 2004
  23. Shawna LM, Lawrence WG, Abby CR, Corinne GH, Laura KK, William HD. Possible lessons from the tobacco experience for obesity control. Am J Clin Nutr 77:1073S-1082S, 2003
  24. Bamia C, Trichopoulou A, Lenas D, Trichopoulos D. Tobacco Smoking in relation to body fat mass and distribution in a general population sample. Int J Obesity 28:1091-1096, 2004 https://doi.org/10.1038/sj.ijo.0802697
  25. Rice-Evans C, Baysal E, Flynn D, Kontoghiiorghes G. Iron-mediated free radical effects on erythrocytes. Biochem Soc Trans 14:368-393, 1986 https://doi.org/10.1042/bst0140368
  26. Marangon K, Herbeth B, Lecomte E, Paul-Dauphin A, Grolier P, Chancerelle Y, Siest G. Diet, antioxidant status, and smoking habits in French men. Am J Clin Nutr 67:231-239, 1998 https://doi.org/10.1093/ajcn/67.2.231
  27. Goldberg B, Stem A. The role of the superoxide anion as a toxic species in the erythrocytes. Achiv Biochem Biophs 178:218-221, 1977 https://doi.org/10.1016/0003-9861(77)90187-4
  28. Kellogg EW, Fridovich I. Liposome oxidation and erythrocyte lysis by enzymatically generated superoxide and hydrogen peroxide. J Biol Chem 252:6721-6726, 1977
  29. Clemens MR, Waller HD. Lipid peroxidation in erythrocytes. Chem Phys Lipids 45:251-257, 1987 https://doi.org/10.1016/0009-3084(87)90068-5
  30. Van Antwerpen VL, Theron AJ, Richard GA. Relationship between the plasma levels of beta-carotene and lung functions in cigarette smokers. Int J Vitamin Nutr Res 65:231-235, 1995
  31. Pamuk ER, Byers T, Coates RJ. Effect of smoking on serum nutrient concentrations in African-American women. Am J Clin Nutr 59:891-895, 1994 https://doi.org/10.1093/ajcn/59.4.891
  32. Tanabe N, Toyoshima H, Hayashi S. Effects of smoking and drinking habits and vitamin A intake on serum concentrations of beta-carotene and retinol. Nippon Eiseigaku Zasshi 47:679-687, 1992 (in Japanese) https://doi.org/10.1265/jjh.47.679
  33. Princen HM, Poppel G, Vogelezang C. Supplementation with vitamin E but not beta-carotene in vivo protects low density lipoproteins from lipid peroxidation in vitro. Arterioscler Thromb 12:554-562, 1992 https://doi.org/10.1161/01.ATV.12.5.554
  34. Albanes D, Virtamo J, Taylor PR, Rautalahti M, Pietinen P, Heinonen OP. Effects of supplemental beta-carotene, cigarette smoking, and alcohol consumption on serum carotenoids in the alpha-tocopherol, beta-carotene cancer prevention study. Am J Clin Nutr 66:366-372, 1997 https://doi.org/10.1093/ajcn/66.2.366
  35. Lykkesfeldt J, Christen S, Wallock LM, Chang HH, Jacob RA, Ames BN. Ascorbate is depleted by smoking and repleted by moderate supplementation: a study in male smokers and nonsmokers with matched dietary antioxidant intakes. Am J Clin Nutr 71:530-536, 2000 https://doi.org/10.1093/ajcn/71.2.530
  36. Rininger I, Chopra M, Thumham DI. Effect of increased fruit and vegetable intake on the susceptibility of lipoprotein to oxidation in smokers. Eur J Clin Nutr 51:601-606, 1997 https://doi.org/10.1038/sj.ejcn.1600451
  37. Handelman GJ, Packer L, Cross CE. Destruction of tocopherols, carotenoids, and retinol in human plasma by cigarette smoke. Am J Clin Nutr 63:559-565, 1996 https://doi.org/10.1093/ajcn/63.4.559
  38. Romanchik JE, Harrison EH, Morel DW. Addition of lutein, lycopene or beta-carotene to LDL or serum in vitro: effects on carotenoid distribution, LDL composition and LDL oxidation. J Nutr Biochem 8:681-688, 1997 https://doi.org/10.1016/S0955-2863(97)00119-8
  39. Steinberg FM, Chait A. Antioxidant vitamin supplementation and lipid peroxidation in smokers. Am J Clin Nutr 68:319327, 1998
  40. Brown AJ. Acute effects of smoking cessation on antioxidant status. J Nutr Biochem 7:29-39, 1996 https://doi.org/10.1016/0955-2863(95)00164-6
  41. Polidori MC, Mecocci P, Stahl W, Sies H. Cigarette smoking cessation increases plasma levels of several antioxidant micronutrients and improves resistance towards oxidative challenge. Br J Nutr 90:147-150, 2003 https://doi.org/10.1079/BJN2003890
  42. Burton GW, Ingold KD. Mechanism of antioxidant action: preventive and chain breaking antioxidants. Ch. 10, In CRC Handbook of free radicals and antioxidants in biomedicine, Miquel 1. ed., pp.29-43, CRC Press, Boca Raton, FL, 1989
  43. Palozza P, Krinsky NI. Astaxanthin and canthaxanthin are potent antioxidants in a membrane model. Archiv Biochem Biophy 297:291-294, 1992 https://doi.org/10.1016/0003-9861(92)90675-M
  44. Terao I. Antioxidant activity of $\beta$-carotene-related carotenoids in solution. Lipids 24:859-865, 1989 https://doi.org/10.1007/BF02535760
  45. Dugas TR, Morel DW, Harrison EH. Impact of carotenoids and alpha-tocopherol content on LDL oxidation by endothelial cells in culture. J Lipid Res 39:999-1007, 1998
  46. Arab L, Steck S. Lycopene and cardiovascular disease. Am J Clin Nutr 71:1691S-1695S, 2000