The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Development of an Efficient Mechanical Inoculation Technique to Screen Barley Genotypes for Resistance to Barley mild mosaic virus Disease and its Comparison to Natural Infection

  • Jonson, Gilda (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Park, Jong-Chul (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Noh, Tae-Hwan (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Mi-Jung (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Hyun, Jong-Nae (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Jong-Gon (Honam Agricultural Research Institute, National Institute of Crop Science, RDA)
  • Published : 2006.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Barley mild mosaic virus(BaMMV) is a soilborne Bymovirus vectored by root-infecting fungus, Polymyxa graminis. Mechanism of cultivar's resistance to BaMMV in field tests are difficult to assess since resistance could be either due to the virus or to P. graminis, or both. Whereas, available mechanical inoculation methods for BaMMV and other related viruses are labor intensive, give inconsistent results and generally result in low infection rates. Inoculation method using stick with gauze(SWG) was developed for BaMMV. The improved method proved to be simple, efficient, and reliable. The infected leaf tissues were preserved by drying in a frozen state under high vaccum(freeze dried barley infected leaves) to circumvent reduction of virus infectivity during storage. Five Korean barley cultivars were mechanically inoculated with BaMMV-infected sap by the improved method. Infection rates obtained were compared with natural infection. Cultivar Naehanssalbori showed resistance to BaMMV in the field trials but was found highly susceptible in the greenhouse tests by mechanical inoculation, indicating that the field resistance may be possibly due to resistance to P. graminis.

Keywords

References

  1. Adams, M. J., Swaby, A. G. and Macfarlane, I. 1986. The susceptibility of barley cultivars to barely yellow mosaic virus (BAYMV) and its fungal vector, Polymyxa graminis. Ann. Appl. Biol. 109:561-571 https://doi.org/10.1111/j.1744-7348.1986.tb03213.x
  2. Adams, M. J. and Jacquier C. 1994. Infection of cereals and grasses by isolates of Polymyxa graminis (Plasmodiophorales). Ann. Appl. Biol. 125:53-60 https://doi.org/10.1111/j.1744-7348.1994.tb04946.x
  3. Freidt, W. 1983. Mechanical transmission of soil-borne barley yellow mosaic virus. Phytopath. Z. 106:16-22 https://doi.org/10.1111/j.1439-0434.1983.tb00023.x
  4. Gardiner, J. M. 1998. University Missouri-Columbia maize RFLP procedures manual. A compilation of procedures with modifications, updated 1998
  5. Huth, W. 1991. Barley yellow mosaic - a disease in Europe caused by two different viruses. In: Viruses with fungal vectors. ed. by J. I. Cooper and M.J. Asher. pp. 61-70. Association of Applied Biologist, UK
  6. Hyun, J. N., Han, S. I., Kim, H. T., Kang, J. R., Ko, J. M., Lim, S. G., Kwon, Y. C., Park, D. S., Suh, D. Y., Kim, D. H., Yoon, C. H., Kim, S. T., Suh, S. J. and Kim, S. C. 2006. A new barley cultivar, 'Hopum' with high quality and BaYMV resistance. Kor. J. Breed. 38:67-68
  7. Kanyuka, K., Ward, E. and Adams, M. J. 2003. Polymyxa graminis and the cereal viruses it transmits: a research challenge. Mol. Plant Pathol. 4:393-406 https://doi.org/10.1046/j.1364-3703.2003.00177.x
  8. Kashiwazaki, S. and Hibino, H. 1996. Genomic reassortment of barley mild mosaic virus: evidence for the involvement of RNA1 in pathogenicity. J. Gen. Virol. 77:581-585 https://doi.org/10.1099/0022-1317-77-4-581
  9. Kashiwazaki, S. Huth, W. and Lessman, D. E. 1998. Barley mild mosaic virus. In: Description of Plant Viruses No. 356. Association of Applied Biologist, U.K
  10. Lee, K., Kashiwazaki, S., Hibi, T. and So, I. 1996. Properties and capsid protein gene sequence of Korean isolate of barley mild mosaic virus. Ann. Phytopath. Soc. Japan 62:397-401 https://doi.org/10.3186/jjphytopath.62.397
  11. Lee, J. L. 1998. Analysis and detection of coat protein gene of barley yellow mosaic virus and barley mild mosaic virus by RT-PCR. Kor. J. Plant Pathol. 14:314-318
  12. Nomura, K., Kashiwazaki, S., Hibino, H., Inoue, T., Nakata, E., Tsuzaki, Y. and Okuyama, S. 1996. Biological and serological properties of strains of barley mild mosaic virus. J. Phytopathol. 144:103-107 https://doi.org/10.1111/j.1439-0434.1996.tb01496.x
  13. Park, J. C., Han, O. K., Hyun, J. N., Kim, M. J. and Kim, J. G. 2005. Degree of field reduction by viral disease following by different resistance. Proceedings of the Annual Meeting of the Korean Society of Crop Science. Kor. J. Crop Sci. 50:178-179
  14. Saha, S., Callahan, F. E., Dollar, D. A. and Creech, J. B. 1997. Effect of lyophilization of cotton tissue on quality of extractable DNA, RNA, and protein. J. Cotton Sci. 1:10-14
  15. Slykhuis, J. T. 1974. Factors critical to mechanical transmissibility of wheat spindle streak mosaic virus. Phytopathology 65:582-584 https://doi.org/10.1094/Phyto-65-582
  16. Zheng, T., Cheng, Y., Chen, J. P., Antoniw, J. F. and Adams, M. J. 1999. The occurrence of barley mild mosaic virus (BaMMV) in China and the nucleotide sequence of its coat protein gene. J. Phytopathol. 147:229-234 https://doi.org/10.1046/j.1439-0434.1999.147004229.x

Cited by

  1. Responses of Resistant Genes to Barley Yellow Mosaic Virus (BaYMV) Strains in Korea vol.15, pp.2, 2009, https://doi.org/10.5423/RPD.2009.15.2.072