생명과학회지 (Journal of Life Science)

  • 제16권7호
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  • Pages.1158-1163
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Bisphenol A 분해균주 Acinetobacter calcoaceticus BP-2의 분리 및 bisphenol A 분해 특성

Isolation of Acinetobacter calcoaceticus BP-2 Capable of Degradation of Bisphenol A

  • 권기석 (안동대학교 생명자원과학부) ;
  • 김동걸 (안동대학교 생명자원과학부) ;
  • 이중복 (안동대학교 생명자원과학부) ;
  • 신기선 (한국생명공학연구원) ;
  • 금은주 (안동대학교 식품영양학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Kwon, Gi-Seok (The School of Bioresource Sciences, Andong National University) ;
  • Kim, Dong-Geol (The School of Bioresource Sciences, Andong National University) ;
  • Lee, Jung-Bok (The School of Bioresource Sciences, Andong National University) ;
  • Shin, Kee-Sun (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kum, Eun-Joo (Department of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 발행 : 2006.12.01
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초록

BPA는 에폭시 수지 및 플라스틱 생산의 단량체로서 사용되어 왔으며, 접착제, 페인트, 광학렌즈, 건축자재, 전자제품 소재 등 다양한 제품을 생산하는 데 사용되고 있다. 그러나 BPA의 급성세포독성 및 내분비교란활성이 보고되면서 BPA의 분해에 대한 연구가 집중되고 있다. 본 연구에서는 BPA의 광분해 및 화학적 분해의 문제점을 극복하고, 실제적 BPA의 생물학적 분해를 목표로 BPA분해균을 플라스틱 공장의 토양으로부터 분리하였다. 분리균주 중 가장 활성이 우수한 BP-2는 5mM의 BPA처리에서 성장할 수 있었으며, pH 7, $30^{\circ}C$의 최적 배양조건에서 $53.3{\mu}g\;ml^{-1}\;day^{-1}$의 분해속도를 나타내었다. 균주 동정결과 BP-2는 Acinetobacter calcoaceticus로 확인되었으며, 3.5g-건조중량$1^{-1}$의 고농도 휴식 세포 반응 결과 $89.7{\mu}g\;ml^{-1}\;h^{-1}$의 BPA분해속도를 나타내었다. 이러한 결과는 고농도 세포농도를 유지하는 경우, BP-2균주가 실제적 BPA분해를 위한 생물촉매로 사용될 수 있음을 제시하고 있다.

Bisphenol A (BPA), 2,2-bis(4-hydroxyphenyl) propane, has been widely used as a monomer for production of epoxy resins and polycarbonate plastics, and final products of BPA include adhesives, protective coatings, paints, optical lens, building materials, compact disks and other electrical parts. Since BPA is a toxic chemical to elicit acute cell cytotoxicity and chronic endocrine disrupting activity, the degradation of BPA has been focused during last decades. To overcome the problem of photo-, and chemical-degradation of BPA, in this study, a bacterium that is able to biodegrade BPA, was isolated. The bacterium, isolated froln the soil of plastic factory, was identified as Acinetobacter calcoaceticus (strain BP-2) based on physiological and 16S rDNA sequencing analysis. A. calcoaceticus BP-2 was able to grow in the presence of $1140{\mu}g\;ml^{-1}$ BPA. Biodegradation experiments showed that BP-2 mineralized BPA via 4-hydroxybenzoic acid and 4-hydroxyacetophenone, and average degradation rate was $53.3{\mu}g\;ml^{-1}\;day^{-1}$ under optimal conditions (pH 7 and $30^{\circ}C$). In high density resting cell $(3.5g-dcw.1^{-1})$ experiments, the maximal degradation rate was increased to $89.7{\mu}g\;ml^{-1}\;h^{-1}$. Our results suggest that BP-2 has high potential as a catalyst for practical BPA bioremediation.

키워드

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