Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Agaricus blazei β-Glucan and Egg Shell Calcium Complex on Bone Metabolism in Ovariectomized Rats

난소절제 흰쥐에서 신령버섯의 β-Glucan과 난각 Ca 복합체가 골 대사에 미치는 효과

  • Noh, Kyung-Hee (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Jang, Ji-Hyun (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Kim, Jin-Ju (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Park, Cherl-Woo (HK Biotech Co., Ltd.) ;
  • Kim, Jeong-Ok (HK Biotech Co., Ltd.) ;
  • Kim, Jae-Cherl (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University) ;
  • Song, Young-Sun (School of Food and Life Sciences, Food Science institute, and Biohealth Product Research Center, Inje University)
  • 노경희 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 장지현 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 김진주 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 박철우 ((주) HK 바이오텍) ;
  • 김정옥 ((주) HK 바이오텍) ;
  • 김재철 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터) ;
  • 송영선 (인제대학교 의생명공학대학 식품생명과학부, 식품과학연구소 및 바이오헬스소재연구센터)
  • Published : 2006.12.29
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Abstract

This study was designed to evaluate the effect of Agaricus blazei $\beta-glucan$ and egg shell calcium complex on bone metabolism in ovariectomized (OVX) rats. Forty Sprague-Dewley female rats, 10 weeks of age $(248{\pm}1.7g)$, were divided into 4 groups and fed on the experimental diets for 6 weeks: sham operated control treated with normal diet containing 0.5% calcium (Sham-C), OVX-control treated with normal diet containing 0.5% calcium (OVX-C), $OVX-\beta-glucan$ group treated with $\beta-glucan$ diet containing 0.5% calcium (OVX-G), and $OVX-\beta-glucan$ egg shell calcium complex treated with $OVX-\beta-glucan$ egg shell calcium complex containing 0.5% calcium (OVX-GE). Bone weight of femur was higher in the OVX-GE group than in the other OVX groups. Bone mineral density of femur was significantly different (p<0.05) among the experimental groups and showed the highest level in the OVX-GE group. Calcium absorption rate and retention were higher in the $\beta-glucan$ supplement groups than in the other groups (p<0.05). Alkaline phosphatase activities and osteocalcin levels of serum showed lower in the $\beta-glucan$ supplement groups than in the OVX-C group. Deoxypyridinoline crosslink values of urine, indicator of bone absorption, showed the lowest in the OVX-GE group. The $\beta-glucan$ supplemented groups had a lower bone resorption ratio than in the OVX-C group. We concluded that bioavailability of calcium is higher in $\beta-glucan$ supplement groups compared to those in OVX rats. From the above results, these findings suggest the possibility of using $\beta-glucan$ egg shell calcium complex as a functional food material related to bone metabolism, even though there is no significant difference between the groups of $\beta-glucan$ and $\beta-glucan-egg$ shell calcium complex supplementation.

본 연구는 칼슘 급원으로서 $\beta-glucan-egg$ shell calcium complex($\beta-glucan-Ca$ 복합체)의 유효성을 확인하여 칼슘 보충제 개발에 활용하고자 실시하였다. 신령버섯에서 추출한 $\beta-glucan$과 난각의 Ca을 결합시켜 $\beta-glucan-Ca$ 복합체로 제조하여 골다공증 실험동물에서 $\beta-glucan$ 및 $\beta-glucan-Ca$ 복합체가 골 대사에 미치는 효과를 비교하였다. 10주령의 Sprague-Dewley 암컷 흰쥐의 양쪽 난소를 절제(OVX)한 후 실험식 이를 급여하였으며 Sham-대조군(Sham-C)은 실험군과 동일한 스트레스를 주기 위해 난소를 절제하지 않고 개복수술만 실시한 후 실험하였다. 식이조성은 Sham-C, OVX-대조군(OVX-C)와 $OVX-\beta-glucan$군(OVX-G)은 $CaCO_3$로 Ca 함량이 0.5%가 되도록 조정하였으며, OYX-G군은 $\beta-glucan$을 첨가하여 제공하였다. $OVX-\beta-glucan-Ca$ 복합체군(OVX-GE)은 $\beta-glucan-Ca$ 복합체의 Ca 농도가 0.5% 함유하도록 조정하여 실험식이를 각각 조제하여 6주간 급여하였다. 체중 100 g당 건조시킨 대퇴골의 무게는 Sham-C>OVX-GE>OVX-C와 OVX-G의 순으로 나타나 $\beta-glucan-Ca$ 복합체를 제공한 OVX-GE에서 다른 OVX군에 비해 상대적으로 골 무게가 증가하는 경향을 보였다. 대퇴골의 골밀도는 각 군 간의 유의적인 차이를 보였으며 Sham-C에 비해 $\beta-glucan$을 첨가한 식이를 제공받은 군에서 골밀도가 상대적으로 높은 수준을 보였고 특히 OVX-GE에서 가장 높은 골밀도를 보였다. Ca체내 흡수율과 보유량은 각 군 간에 유의적인 차이를 보였으며 OVX-C가 Sham-C에 비해 높았으며 OVX군에서는 $\beta-glucan$를 첨가한 군이 높은 수준을 보였다. ALP의 활성은 OVX군에서는 $\beta-glucan$을 첨가한 군이 다소 낮은 경향을 보였다. 요 중의 골 흡수 지표인 DPD crosslink value는 각 군 간의 유의적인 차이를 보였고 OVX군의 DPD crosslink value가 Sham-C에 비해 현저하게 높은 수준으로 나타나 OVX군의 골 흡수가 높음을 확인할 수 있었다. OVX군에서 $\beta-glucan$을 첨가한 식이를 제공한 OVX-G와 OYX-GE의 DPD crosslink value가 OVX-C에 비해 유의적으로 낮은 수준이었으며 $\beta-glucan$이 난소 절제 흰쥐 모델에서 골 대사에 영향을 미쳐 골 흡수율을 낮추어 골 보호효과를 나타내는 것으로 보인다. 이상의 결과들로 미루어 볼 때, 비록 $\beta-glucan$을 제공한 군과 $\beta-glucan-Ca$ 복합체를 제공한 두 군 간에는 유의적인 차이를 보이지 않았으나 난소절제 흰쥐모델의 골 대사에서 $\beta-glucan-Ca$복합체 형태가 골다공증 예방을 위한 기능성 식품으로서의 가능성을 보여주었으며, 앞으로 골다공증 예방을 위한 $\beta-glucan-Ca$ 복합체 형태의 효과적인 농도는 계속적으로 연구가 진행되어야 할 것으로 사료된다.

Keywords

References

  1. Lee JW, Kim HJ, Jhee OH, Won HD, Yu YJ, Lee MH, Kim TW, OM AS, Kang JS. 2005. Effects of alternative medicine extract on bone mineral density, bone strength and biochemical markers of bone metabolism in ovariectomized rats. Korean J Food Nutr 18: 72-80
  2. Lee YB, Lee HJ, Kim KS, Lee JY, Nam SY, Cheon SH, Sohn HS. 2004. Evaluation of the preventive effect of isoflavone extract on bone loss in ovariectomized rats. Biosci Biotechnol Biochem 68: 1040-1045 https://doi.org/10.1271/bbb.68.1040
  3. Byun JS, Rho SN, Park JS, Park HM. 2005. Effect of isoflavone supplementation on bone metabolism in ovariectomized rats at different ages. J Korean Soc Food Sci Nutr 34: 1350-1356 https://doi.org/10.3746/jkfn.2005.34.9.1350
  4. The Korean society of bone metabolism. 1997. Osteoporosis. Seoul, Korea
  5. Seo BI. 2003. A study on Agaricus blazei. J Applied Oreintal Medicine 3: 83-89
  6. Sung JM, Yoo YB, Cha DY. 1998. Mushroom. Kuohaksa, Seoul. p 3-10
  7. Sugjyama K, Saeki S, Ishiguro Y. 1992. Hypercholesterolemic activity of ningyotake (Polyporus confluences) mushroom in rats. J Jpn Soc Nutr Food Sci 45: 265-270 https://doi.org/10.4327/jsnfs.45.265
  8. Yoshiaki F, Hidekazu K, Koichi O, Ryo S, Takusaburo E. 1998. Tumoricidal activity og high molecular weight polysaccharides derived from Agaricus blazei via oral administration in the mouse tumor model. Nippon Shokuhim Kagaku Kaishi 45: 246-252 https://doi.org/10.3136/nskkk.45.246
  9. Choi JM, Koo SJ. 2000. Effects of $\beta$-glucan from Agaricus blazei Murill on blood glucose and lipid composition in db/db mice. Korean J Food Sci Technol 32: 1418-1425
  10. Itoh H, Amano H, Noda H. 1994. Inhibitory action of a (1$\rightarrow$6)-$\beta$-D-glucan-protein complex isolated from Adaricus blazei Murill on metha fibrosarcoma-bearing mice and its antitumor mechanism. Jpn J Pharmacol 66: 265-271 https://doi.org/10.1254/jjp.66.265
  11. Chung HK, Chang NS, Lee HS, Chang YE. 1996. The effect of various types of calcium sources on calcium and bone metabolism in rats. Korean J Nutrtion 29: 480-488
  12. Recker RR, Bammi A, Barger-Lux J, Heaney RP. 1988. Calcium absorbability from milk products, and imitation milk and calcium carbonate. Am J Clin Nutr 47: 93-95 https://doi.org/10.1093/ajcn/47.1.93
  13. AOAC. 2000. Official Method of Analysis. 17th ed. Association of Analytical Communities, Arlington VA, USA
  14. Zeni S, Gornez-Acotto C, Di Gregorio S, Mautalen C. 2000. Differences in bone turnover and skeletal response to thyroid hormone treatment between estrogen-depleted rats. Calcif Tissue Int 67: 173-177 https://doi.org/10.1007/s00223001106
  15. Erickson GF. 1996. The ovary: basic principles and concepts. In Endocrinology and Metabolism. 3rd ed. Felig P, Baxer JD, Frohman LA, eds. McGrawHill Inc, New York, USA. p 973-1015
  16. Knott L, Bailey AJ. 1998. Collagen crosslinks in mineralizing tissues: A review of their chemistry, function, and clinical relevance. Bone 22: 181-187 https://doi.org/10.1016/S8756-3282(97)00279-2
  17. Longlands MG, Wiener K. 1978. Anomalous behavior of control sera in automated versions of the Kind and King alkaline phophatase method. Ann Clin Biochem 15: 164-167 https://doi.org/10.1177/000456327801500136
  18. Chasson AL, Grady HT, Stanley MA. 1961. Determination of creatinine by means of automatic chemicals analysis. Am J Clin Pathol 35: 83-89 https://doi.org/10.1093/ajcp/35.1_ts.83
  19. Bray DL, Briggs CM. 1984. Decrease in bone density in young male guinea pigs fed high levels of ascorbic acid. J Nutr 114: 920-928 https://doi.org/10.1093/jn/114.5.920
  20. Choi SJ, Kim MK. 2003. Effect of grape intake on cadmium metabolism of rats during aging. Korean J Nutrition 36: 997-1012
  21. Frolik CA, Bryant HU, Black EC, Magee DE, Chandrasekhar S. 1996. Time-dependent changes in biochemical bone markers and serum cholesterol in ovariectomized rats: Effects of raloxifene HCl, tamoxifen, estrogen and alendronate. Bone 18: 621-627 https://doi.org/10.1016/8756-3282(96)00085-3
  22. Kalu DN, Arjmandi BH, Liu CC, Salih MA, Bimbaum RS. 1994. Effects of ovariectomy and estrogen on the serum levels of insulin-like growth factor-1 and insulin-like growth factor binding protein-3. Bone Miner 25: 135-148 https://doi.org/10.1016/S0169-6009(08)80255-3
  23. Lee YB, Lee HJ, Kim KS, Lee JY, Nam SY, Cheon SH, Shon HS. 2004. Evaluation of the preventive effect of isoflavone extract on bone loss in ovariectomized rats. Biosci Biotechnol Biochem 68: 1040-1045 https://doi.org/10.1271/bbb.68.1040
  24. Okasaki R, Inoue D, Shibata M, Saika M, Kido S, Ooka H, Tomiyama H, Sakamoto Y, Matsumoto T. 2002. Estrogen promotes early osteoblast differentiation and inhibits adipocyte differentiation in mouse bone marrow stromal cell lines that express estrogen receptor (ER) $\alpha$ or $\beta$. Endocrinology 143: 2349-2356 https://doi.org/10.1210/en.143.6.2349
  25. O JH, Lee YS. 1993. Effects of dietary calcium levels on the reduction of calcium availability in ovariectomized osteoporosis model rats. Korean J Nutrition 26: 277-285
  26. Nordin BEC, Wishart JM, Clifton PM, MaArthur R, Scopacasa F, Need AG, Morris HA, O'Loughlin PD, Horowitz M. 2004. A longitudinal study of bone-related biochemical changes at the menopause. Clin Endocrinology 61: 123-130 https://doi.org/10.1111/j.1365-2265.2004.02066.x
  27. Lim SK. 1994. Clinical significance and application of bone turnover marker. Korean J Bone Metabolism 1: 1-11
  28. Lee JA, Noh SH, Ahn DK, Choi HY. 2001. Effects of the Eucommiae cotrtex and Chanenomelis fructus on the aged ovariectomized rat of postmenopausal osteoporosis. Kor J Herbology 16: 201-206
  29. Moon SJ, Kim JH, Lim SK. 1996. Investigation of risk of low serum 25-hydroxyvitamin D levels in Korean menopausal women. Korean J Nutrition 29: 981-990
  30. Aloia JF, Cohr SH, Vaswani A, Yeh JK, Yuen K, Ellis K. 1985. Risk factors for postmenopausal osteoporosis. Am J Med 78: 95-100 https://doi.org/10.1016/0002-9343(85)90468-1
  31. Price PA, Pathermore JG, Doftos LJ. 1980. New biochemical marker for bone metabolism. J Clin Invest 66: 878-883 https://doi.org/10.1172/JCI109954
  32. Sarioglu M, Tuzun C, Unlu Z, Tikiz C, Taneli F, Uvanik BS. 2006. Comparison of the effects of alendronate and risedronate on bone mineral density and bone turnover markers in postmenopasual osteoporosis. Rheumatol Int 26: 195-200 https://doi.org/10.1007/s00296-004-0544-z
  33. Erye DR. 1996. Biochemical markers of bone turnover. In Primer on the metabolic bone diseases and disorder of mineral metabolism. 3rd ed. Favus MJ, ed. Raven Press, New York. p 114-118
  34. Uebelhart D, Gineyts E, Chapuy MC, Delmas PD. 1990. Urinary excretion of pyridium crosslinks: a new marker of bone resorption in metabolic bone disease. Bone Mineral 8: 87-96 https://doi.org/10.1016/0169-6009(91)90143-N
  35. Seibel MJ, Gartenberg SJ, Ratcliffe A, Robins SP, Bilezikian JP. 1992. Urinary hydroxypyridium crosslinks of collagen in primary hyperparathyroidism. J Clin Endocrinol Metab 74: 481-486 https://doi.org/10.1210/jc.74.3.481
  36. Christel P, Catherine BP, Brigitte C, Patrice L, Marie- Jeanne D, Jean-Pierre B, Veronique C. 2001. Soybean isoflavones dose-dependently reduce bone turnover but do not reverse established osteopenia in adult ovariectomized rats. J Nutr 131: 723-728
  37. Notelovitz M. 1993. Osteoporosis: screening, prevention and management. Fertility Sterility 59: 714-715
  38. Choi MJ, Yu TS. 2004. Effects of red-yeast-rice supplementation on bone mineral density and bone mineral content in ovariectomized rats. Korean J Nutrition 37: 423- 430

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