Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Study of the Presence of Residual Hexane in Olive Oils

유통 올리브유의 잔류 헥산에 대한 연구

  • Kim, Nam-Sook (Dept. of food Science and Technology, Chungnam National University) ;
  • Lee, Jeung-Hee (Dept. of food Science and Technology, Chungnam National University) ;
  • Heo, Ok-Soon (Daejeon Korea Food & Drug Administration) ;
  • Lee, Ki-Teak (Dept. of food Science and Technology, Chungnam National University)
  • Published : 2006.12.29
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Abstract

The presence and content of residual hexane in the olive oils were studied. Total 41 olive oils of imported and domestic brands, which were labeled as extra virgin and refined (mixed), were collected from the market. For analysis, electronic nose and headspace SPME-GC/MS were used. Electronic nose equipped with 12 metal oxide sensors was used for the discrimination of odor pattern of olive oils against the different concentrations of hexane. From the results, it is assumed that the contents of residual hexane in the collected olive oils were below 5 ppm. For Qualitative and quantitative analysis of hexane, polydimethylsiloxane (PDMS) fiber was employed for SPME-GC/MS. In the results, the peak of residual hexane was detected in 8 samples from 41 olive oils. But the detected level was no more than 1 ppm that is under the regulation limit (5 ppm) by Korea Food Additive Code.

국내에서 유통되는 국내외 브랜드의 extra virgin급과 정제/혼합 올리브유에 잔류된 헥산의 함량을 측정하기 위하여 신속하고 간편한 headspace법을 이용한 전자코(a-FOX 3000, Alpha M.O.S., France)를 이용하여 분석하였으며, head-space-SPME 법을 사용한 GC/MS 분석방법을 이용하여 정량하였다. 전자코 분석결과, 올리브유에 함유된 헥산의 존재 유무 및 농도를 예측할 수 있을 것으로 사료되었으며, 1차로 수거된 국내에 유통 중인 5종의 국내 브랜드 extra virgin급, 10종의 국외브랜드 extra virgin급과 7종의 국내외 브랜드인 혼합/정제 올리브유의 경우 헥산의 잔류 합유량은 잔류되어 있지 않거나, 잔류되어 있다고 하더라도 국내 허용치인 5 ppm 미만으로 예측되었다. 잔류 헥산을 정량하기 위하여 polydimethylsiloxane(PDMS)으로 코팅된 fiber를 사용한 headspace SPME-GC/MS를 사용하였다. 이 때 시료의 head space산출을 위한 최적의 휘발촉진 온도를 선정하기 위하여 각 온도별 검량선 작성한 결과 $R^2$값이 99.99%로 가장 재현성이 높을 것으로 판단되는 $70^{\circ}C$에서 30 min 동안의 휘발촉진과정을 통하여 휘발성분을 fiber에 흡착하였다. 1차로 수집한 총 22개의 올리브유 중 총 6개의 시료에서 헥산이 검출되었고, 2차로 수집한 총 19개의 올리브유에서는 총 2개의 시료에서 헥산이 검출되었으나, 검출된 잔류 헥산의 양은 모두 국내 규정한계농도(5 ppm) 이하인 1 ppm 이하인 것으로 확인되었다.

Keywords

References

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