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Identification and Physiological Characteristics of Microorganism Isolated from Spoiled Sweetened Adzuki Ann

부패된 팥앙금으로부터 분리된 미생물의 동정과 그 균의 생리적 특성

  • 이태규 (우석대학교 대학원 생명공학과) ;
  • 노민환 (우석대학교 대학원 생명공학과)
  • Published : 2006.12.29

Abstract

In order to determine the causes of sweetened adzuki ann spoilage, the characteristics of microorganism isolated from spoiled adzuki ann were investigated. The isolated microorganism was gram-positive, roil-shaped and shore-forming bacteria; its surface was smooth and glazed. From the results of the assimilation test of 46 different biochemicals by the Vitec 2 Compact test and comparison of the cellular wall composition of fatty acid by the data bank of Midi sherlock system, the microorganism was identified as Bacillus subtilis, D-value of the B. subtilis spore was 4.85 min at $115^{\circ}C$, 0.69 min at $121^{\circ}C$ and 0.48 min at $125^{\circ}C$; Z-value was 9.71. The Bacillus subtilis growth was not observed below water activity of 0.92 at $45^{\circ}C$. However, bacteria growth increased gradually as water activity increased above 0.93.

변질된 팥앙금에서 분리한 균주는 광학 및 전자현미경을 통하여 관찰한 결과 그람양성 간균으로 포자를 형성하고 형성된 집락은 원형으로 점질성의 물질을 생산하는 mucoid type이었으며, 표면은 smooth 하였고 광택을 나타내었다. 미생물 동정 시스템($MicroLog^{TM}$ System, Release 4)인 VITEK 2 COMPACT을 이용하여 분리된 균주의 46개의 생화학적 분석을 비교한 결과 팥앙금의 부패 원인균은 Bacillus subtilus로 동정 (97.0%)되었으며, 보다 더 정확한 동정을 위하여 부패균의 세포벽 지방산 조성을 분석하여 Midi sherlock시스템의 databank와 비교 분석시 그 유사성이 0.724로 분석되었다. Bacillus subtilus의 포자의 D-value는 $115^{\circ}C$에서의 4.85분이 었고, $121^{\circ}C$에서는 0.69분, $125^{\circ}C$에서는 0.48분이었으며, Z-value는 $9.71^{\circ}C$이었다. $45^{\circ}C$에서 수분활성도에 따른 분리균의 생육 상태를 관찰한 결과 수분활성도 0.92이하에서는 균이 증식되지 않았고, 0.94에서는 증식되는 것으로 관찰되었다.

Keywords

References

  1. Cho JY. 1986. 田作. Hyeomunsa Press, Seoul, Korea. p 330-332
  2. Song JC. 2000. Food Material. Kyomunsa, Seoul, Korea. p 247-248
  3. Kim CS, Chung SK, Oh YK, Kim RY. 2003. Antimicrobial activity of green tea against microrganism in steamed bread. J Korean Soc Food Sci Nutr 32: 413-417 https://doi.org/10.3746/jkfn.2003.32.3.413
  4. Hwang CS, Kim HH, Oh BC, Kim YS, Shin DH. 2004. Identification and characteristics of microorganism isolated from spoiled red bean paste. Food Sci Biotechnol 13: 758- 761
  5. Natio S. 1996. あんの製造に關する徵生物管理について. J Japanese Soc Food Engineering 16: 160-171
  6. Wallet F, Loïez C, Renaux E, Lemaitre N, Courcol RJ. 2005. Performances of VITEK 2 colorimetric cards for identification of gram-positive and gram-negative bacteria. J Clin Microbiol 43: 4402-4406 https://doi.org/10.1128/JCM.43.9.4402-4406.2005
  7. Yoon SH, Kim JB, Lim YH, Hong SR, Song JK, Kim SS, Kwon SW, Park IC, Kim SJ, Yeo YS, Koo BS. 2005. Isolation and characterization of three kinds of lipopeptides produced by Bacillus sutilis JKK238 from Jeot-Kal of Korea traditional fermented fishes. Kor J Microbiol Biotechnol 33: 295-301
  8. John GH, Kreig NR, Sneath PHA. 1994. Bergey's Manual of Systematic Bacterilogy. 9th ed. Williams & Wilkins, MD, USA
  9. Fernandez PS, Go'mez FJ, Ocio MJ, Rodrigo M, Sanchez T, Martinez A. 1995. D value of Bacillus stearothermophillus spores as a funtion of pH and recovery medium acidulant. J Food Prot 58: 628-632 https://doi.org/10.4315/0362-028X-58.6.628
  10. Koo YJ, Lee SD, Shin DH, Yu TJ. 1981. Studies on thermal resistance of selected yeast strain for parsteurization of solid packed peach. Korean J Food Sci Technol 13: 43-52
  11. Hur JK, Lee SJ, Oh SJ, Kim SK, Baek YJ. 1993. Physiology characteristics and heat resistance of yeasts isolated from stirred yogurt. Korean J Dairy Sci 15: 56-65
  12. Doores S, Westhoff D. 1981. Heat resistance of Sporolactobacillus inulinus. J Food Sci 46: 810-812 https://doi.org/10.1111/j.1365-2621.1981.tb15353.x
  13. Shull JJ, Cargo GT, Ernst RR. 1963. Kinetics of activation of thermal death of bacterial spores. Appl Microbiol 11: 485-487
  14. Lee KH, Chang KH. 1961. Studies on thermal resistance bacteria. (Part. 1) On the thermal resistance of aerobic bacteria. J Kor Microbiol 3: 11-14
  15. Kim DY, Kwon YJ, Yang HC. 1990. Food Chemistry. Youngimunhwasa Press, Seoul, Korea. p 27
  16. Adul Ghani AG, Faird MM, Chen XD. 2002. Theoretical and experimental investigation of the thermal inactivation of Bacillus stearothermophilus in food pouches. J Food Engineering 51: 221-228 https://doi.org/10.1016/S0260-8774(01)00060-7
  17. Stumbo CR. 1973. Thermobactoeriology in food processing. 2nd ed. Academic Press, New York. p 113
  18. Condo'n S, Palop A, Raso J, Sala FJ. 1996. Influence of the incubation temperature after heat treatment upon the estimated heat resistance value of spores of Bacillus subtilis. Lett Appl Microbiol 22: 149-152 https://doi.org/10.1111/j.1472-765X.1996.tb01130.x
  19. Condo'n S, Lopez P, Oria R, Sala FJ. 1989. Thermal death determination: design and evaluation of a thermoresistometer. J Food Sci 54: 451-457 https://doi.org/10.1111/j.1365-2621.1989.tb03104.x
  20. Yokoya F, York GK. 1965. Effect of several environment conditions on the 'thermal death rate' of endospores of aerobic, thermophilic bacteria. Appl Microbiol 13: 993-999