Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Inhibition of Gap Junctional Intercellular Communication by Food Preservatives Potassium Sorbate

소르빈산 칼륨의 GJIC 억제로 인한 간독성 유발

  • Hwang, Jae-Woong (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Chung, Ji-Hye (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Ji-Won (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Sun-Jung (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jung-Ran (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Ahn, Ji-Yun (Food Function Research Division, Korea Food Research Institute) ;
  • Ha, Tae-Youl (Food Function Research Division, Korea Food Research Institute) ;
  • Kim, Sung-Ran (Food Function Research Division, Korea Food Research Institute) ;
  • Lee, Yong-Soon (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-Sun (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • 황재웅 (서울대학교 수의과대학 수의공중보건학교실) ;
  • 정지혜 (서울대학교 수의과대학 수의공중보건학교실) ;
  • 정지원 (서울대학교 수의과대학 수의공중보건학교실) ;
  • 정지윤 (공주대학교 산업과학대학 특수 동물학과) ;
  • 김선중 (서울대학교 수의과대학 수의공중보건학교실) ;
  • 박정란 (서울대학교 수의과대학 수의공중보건학교실) ;
  • 안지윤 (한국식품연구원) ;
  • 하태열 (한국식품연구원) ;
  • 김성란 (한국식품연구원) ;
  • 이영순 (서울대학교 수의과대학 수의공중보건학교실) ;
  • 강경선 (서울대학교 수의과대학 수의공중보건학교실)
  • Published : 2006.12.30
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Abstract

Potassium Sorbate (PS) is a potassium salt version of sorbic acid, which has antimicrobial and fungistatic features in foods. Therefore, PS is used as a food preservative against fungi and mold. PS has been found to be non-toxic even when taken in large quantities given its trait to be broken down in the body into water and carbon dioxide. Gap Junctional Intercellular Communication (GJIC) is essential in the maintenance of tissue homeostasis during development and differentiation. This study was made of the effects of PS on GJIC in WB-F344 rat liver epithelial (WB) cells. We found dramatic decrease of cell viability in time- and dose-dependent manners when WB cells were treated with PS. The effect of PS on GJIC is strong inhibition, and it took place in parallel with a hyperphosphorylation of connexin 43 expression. The finding that PS interferes with gap junction functionality should be considered with respect to the mechanism of PS-induced hepatotoxicity.

소르빈산 칼륨은 소르빈산의 일종으로 항세균 및 곰팡이 발육 저지 효과를 갖고 있다. 이러한 성질로 인하여 소르빈산 칼륨은 곰팡이와 진균에 대한 식품 방부제로서 사용되고 있다. 소르빈산 칼륨은 많은 양에 노출되었을 때도 체내에서 수분과 이산화탄소로 분해되는 특성으로 인하여 독성이 없는 것으로 알려져 있다. gap junction을 통한 세포 간 신호 전달(GJIC)는 생체의 성장과 분화에 있어서 조직의 항상성 유지에 본질적인 역할을 하고 있다. 본 연구는 WB-F344 랫드의 정상 간 상피 세포(WB 세포)에서 소르빈산 칼륨을 노출하였을 때 GJIC에 대하여 어떤 영향을 미치는지 알아보았다. 그 결과 소르빈산 칼륨에 노출에 의하여 WB세포의 GJIC가 농도 및 시간 의존적으로 현격하게 감소하는 것을 관찰하였다. 소르빈산 칼륨은 GJIC를 강력하게 억제하며, 이는 WB 세포에서 gap junction을 구성하는 connexin 43의 인산화와 병행하는 것을 확인하였다. 소르빈산 칼륨이 gap junction의 기능을 방해함으로 인해 소르빈산 칼륨에 의한 간독성이 유발될 수 있을 것이다.

Keywords

References

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