Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Portulaca Oleracea L. Extract on Lipolysis and Hormone Sensitive Lipase (HSL) Gene Expression in 3T3-L1 Adipocytes

마치현 추출물이 3T3-L1 지방세포에서 지방 분해 및 Hormone Sensitive Lipase (HSL) 유전자 발현에 미치는 효과

  • Lee, Mak-Soon (Department of Food and Nutritional Sciences, Ewha Womans University) ;
  • Kim, Chong-Tai (Biosystems Engineering Team, Korea Food Research Institute) ;
  • Kim, Chul-Jin (Biosystems Engineering Team, Korea Food Research Institute) ;
  • Cho, Young-Jin (Biosystems Engineering Team, Korea Food Research Institute) ;
  • Kim, Yang-Ha (Department of Food and Nutritional Sciences, Ewha Womans University)
  • 이막순 (이화여자대학교 식품영양학과) ;
  • 김종태 (한국식품연구원 시스템공학연구팀) ;
  • 김철진 (한국식품연구원 시스템공학연구팀) ;
  • 조용진 (한국식품연구원 시스템공학연구팀) ;
  • 김양하 (이화여자대학교 식품영양학과)
  • Published : 2006.12.31
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Abstract

This study was performed to investigate the lipolytic effects of Portulaca oleracea L. extract in 3T3-L1 adipocytes. The Portulaca oleracea L. was extracted with extrusion method using twin-screw extruder under $58{\sim}60rpm$ screw speed, $4{\sim}5kg/hr$ feed rate, $140^{\circ}C$ extrusion temperature. The lipolytic action of Portulaca oleracea L. extract was estimated by measuring the amount of glycerol and free fatty acids (FFA) released from 3T3-L1 adipocytes and by measuring the cellular lipid content in 3T3-L1 adipocytes. The hormone sensitive lipase (HSL) mRNA level was analyzed using quantitative real-time PCR. The Portulaca oleracea L. extract at 1 to $100{\mu}g/ml$ suppressed lipid accumulation. The release of glycerol and FFA into the medium, and the mRNA level of HSL were significantly increased by the addition of Portulaca oleracea L. extract at dose-dependent manner. In conclusion, the Portulaca oleracea L. extract was suggested to have the lipolytic effect through release of lipolytic products (FFA and glycerol) of triacylglyceride to the culture medium and suppression of lipid accumulation via up-regulation of HSL gene expression in 3T3-L1 adipocytes.

Keywords

References

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