Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND ITS RECEPTORS IN THE DISTRACTED PERIOSTEUM AFTER MANDIBULAR DISTRACTION OSTEOGENESIS

하악골 신장술 후 신생 골막조직에서의 혈관내피세포성장인자 및 혈관내피세포성장인자 수용체 발현에 대한 연구

  • Hwang, Deung-Uc (Department of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Byun, June-Ho (Department of Oral & Maxillofacial Surgery, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Park, Bong-Wook (Department of Oral & Maxillofacial Surgery, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Jong-Ryoul (Department of Oral & Maxillofacial Surgery, College of Dentistry, Pusan National University)
  • 황등욱 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 변준호 (경상대학교 의과대학 치과학교실 구강악안면외과, 경상대학교 의과대학 건강과학연구원) ;
  • 박봉욱 (경상대학교 의과대학 치과학교실 구강악안면외과, 경상대학교 의과대학 건강과학연구원) ;
  • 김종렬 (부산대학교 치과대학 구강악안면외과학교실)
  • Published : 2006.12.31
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Abstract

During distraction osteogenesis, the angiogenic activity is crucial factor in the new bone formation. The aim of this study was to detect the autocrine growth activity in the cellular components of the distracted periosteum with observation of the expression of vascular endothelial growth factor (VEGF) and its receptors following the mandibular distraction osteogenesis. Unilateral mandibular distraction (0.5 mm twice per day for 10 days) was performed in six mongrel dogs. Two animals were sacrificed at 7, 14, and 28 days after completion of distraction, respectively. The distracted lingual periosteum was harvested and processed for immunohistochemical examinations. After then, we observed the expression of VEGF, Flt-1 (VEGFR-1), and Flk-1 (VEGFR-2) in the osteoblasts and immature mesenchymal cells of the distracted periosteum. At 7 days after distraction, the expression of VEGF and its receptors were significantly increased in the cellular components of the distracted periosteum. Up to 14 days following distraction, the increased expressions were maintained in the osteoblastic cells. At 28 days after distraction, the expression of VEGF and its receptors decreased, but VEGF was still expressed weak or moderate in the osteoblastic cells of distracted periosteum. The expression pattern of VEGF and its receptors shown here suggested that VEGF play an important role in the osteogenesis, and these osteoblastic cell-derived VEGF might act as autocrine growth factor during distraction osteogenesis. In the other word, the cellular components in the distracted periosteum, such as osteoblasts and immature mesenchymal cells, might have autocrine growth activity during distraction osteogenesis.

Keywords

References

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