Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Antitumor Activity of Cell Suspension Culture of Green Tea Seed (Camellia sinensis L.)

  • Choi, Jae-Hoon (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Yoon, Sang-Kun (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Lee, Keyong-Ho (Kolon Central Research Park) ;
  • Seo, Min-Soo (Hankooktea Company, Honam Tea Farm) ;
  • Kim, Doo-Hwan (Department of Molecular Biotechnology, Konkuk University) ;
  • Hong, Seung-Beom (Department of Molecular Biotechnology, Konkuk University) ;
  • Kim, Ji-Yeon (Department of Animal Science, Woosong Information College) ;
  • Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Kim, Chang-Han (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
  • Published : 2006.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this study was to investigate the antitumor activity of suspension cultures of tea callus cells grown in the presence of different concentrations of the growth regulator 2,4-dichlorophenoxy acetic acid (2,4-D) with or without light irradiation. The methanol and ethanol extracts of precipitated cells (MEP, EEP) exhibited stronger inhibitory effects on the growth of tumor cell lines than the water extract of precipitated cells (WEP) or the supernatant Compared to culture under dark conditions, exposure to light irradiation led to significantly higher antitumor activity. The MEP from light irradiated cells at $250{\mu}g/mL$ with 2.0mg/L 2,4-D displayed more than 64% growth inhibition of HEP-2 cells, whereas normal cells showed less than 25% growth inhibition. The some fractions of MEP obtained from Diaion HP-20 column chromatography displayed the majority of inhibitory activity against the HEP-2 cell line. These results show that 2,4-D, and light stimulated the synthesis of antitumor compounds.

Keywords

References

  1. Furuya, T., Y. Origara, and Y. Tsuda (1990) Caffeine and theanine from cultured cells of Camellia sinensis. Phytochemistry 29: 2539-2543 https://doi.org/10.1016/0031-9422(90)85184-H
  2. Millin, D. J. (1987) Factors affecting quality of tea. pp. 127-160. In: S. M. Herschduerter (eds.). Quality Control in the Food Industry. Academic Press, London, England
  3. Haider, B., S. Pramanick, S. Mukhopadhyay, and A. K. Giri (2005) Inhibition of benzo[a]pyrene induced mu-tagenicity and genotoxicity by black tea polyphenols theaflavins and thearubigins in multiple test systems. Food Chem. Toxicol. 43:591-597 https://doi.org/10.1016/j.fct.2005.01.002
  4. Chung, S. K., M. Y. Kim, Y. C. Kim, K. Iwai, and H. Matsue (2004) Antioxidant effects of Korean teabag teas by a simple and fast XYZ-dish method. Food Sci. Biotechnol. 13: 197-201
  5. Mabe, K., M. Yamada, I. Oguni, and T. Takahashi (1999) In vitro and in vivo activities of tea catechins against Helicobacter pylori. Antimicrob. Agents Chemother. 43: 1788-1791
  6. Sakanaka, S., L. R. Juneja, and M. Taniguchi (2000) Antimicrobial effects of green tea polyphenols on thermophilic spore-forming bacteria. J. Biosci. Bioeng. 90: 81-85 https://doi.org/10.1016/S1389-1723(00)80038-9
  7. Li, X. C., H. N. ElSohly, A. C. Nimrod, and A. M. Clark (1999) Antifungal activity of (-)-epigallocatechin gallate from Coccoloba dugandiana. Planta Med. 65: 780 https://doi.org/10.1055/s-2006-960871
  8. Calzada, F., M. Meckes, and R. Cedillo-River (1999) An-tiamoebic and antigardial activity of plant flavonoids. Planta Med. 65: 78-80 https://doi.org/10.1055/s-2006-960445
  9. Yang, C. S., J. Y. Chung, G. Y. Yang, S. K. Chhabra, and M. J. Lee (2000) Tea and tea polyphenols in cancer prevention. J. Nutr. 130: 472S-478S
  10. Cao, Y. and R. Cao (1999) Angiogenesis inhibited by drinking tea. Nature 398: 381 https://doi.org/10.1038/18793
  11. Ogutuga, D. B. A. and D. H. Northcote (1970) Caffeine formation in tea callus tissue. J. Exp. Bot. 21: 258-273 https://doi.org/10.1093/jxb/21.2.258
  12. Takeo, T. (1974) L-Alanine as a precursor of ethylamine in Camellia sinensis. Phytochemistry 13: 1401-1406 https://doi.org/10.1016/0031-9422(74)80299-2
  13. Matsuura, T. and T. Kakuda (1990) Effects of precursor temperature and illumination on theanine accumulation in tea callus. Agric. Biol. Chem. 54: 2283-2286
  14. Shervington, A., L. A. Shervington, F. Afifi, and M. A. El-Omari (1998) Caffeine and theobromine formation by tissue cultures of Camellia sinensis. Phytochemistry 47: 1535-1536 https://doi.org/10.1016/S0031-9422(97)01087-X
  15. Yoon, W. H., J. H. Choi, K. H. Lee, and C. H. Kim (2005) Antimicrobial and antitumor activities of seed extracts of Camellia sinensis L. Kor. J. Food Sci. Technol. 37: 108-112
  16. Sur, P. and D. K. Ganguly (1994) Tea plant root extract (TRE) as an antineoplastic agent. Planta Med. 60: 106-109 https://doi.org/10.1055/s-2006-959427
  17. Chaudhuri, T., P. Sur, A. Gomes, S. K. Das, M. Das, and D. K. Ganguly (1998) Effect of tea root extract (TRE) on solid tumours induced by 3-methylcholanthrene in mice. Phytother. Res. 12: 62-64 https://doi.org/10.1002/(SICI)1099-1573(19980201)12:1<62::AID-PTR184>3.0.CO;2-4
  18. Das, M., P. Sur, A. Gomes, J. R. Vedasiromoni, and D. K. Ganguly (2002) Inhibition of tumor growth and inflammation by consumption of tea. Phytother. Res. 16: 40-44 https://doi.org/10.1002/ptr.797
  19. Gupta, S., K. Hastak, N. Ahmad, J. S. Lewin, and H. Mukhtar (2001) Inhibition of prostate carcinogenesis in TRAMP mice by oral infusion of green tea polyphenols. Proc. Natl. Acad. Sci. USA 98: 10350-10355
  20. Kuo, P. L. and C. C. Lin (2003) Green tea constituent (-) -epigallocatechin-3-gallate inhibits Hep G2 proliferation and induces apoptosis through p53-dependent and fas-mediated pathways. J. Biomed. Sci. 10: 219-227
  21. Yang, C. S., P. Maliakal, and X. Meng (2002) Inhibition of carcinogenesis by tea. Annu. Rev. Pharmacol. Toxicol. 42: 25-54 https://doi.org/10.1146/annurev.pharmtox.42.082101.154309
  22. Prakash, G., C. J. S. K. Emmannuel, and A. K. Srivastava (2005) Variability of azadirachtin in Azadirachta indica (neem) and batch kinetics studies of cell suspension culture. Biotechnol. Bioprocess Eng. 10: 198-204 https://doi.org/10.1007/BF02932013
  23. Park, Y. G., S. J. Kim, H. Y. Jung, Y. M. Kang, S. M. Kang, D. T. Prasad, S. W. Kim, and M. S. Choi (2004) Variation of ginkgolides and bilobalide contents in leaves and cell cultures of Ginkgo biloba L. Biotechnol. Bioprocess Eng. 9: 35-40 https://doi.org/10.1007/BF02949319
  24. Strekova, V Y., N. V. Zagoskina, G. A. Subbotina, and M. N. Zaprometev (1989) Effect of prolonged illumination on synthesis of phenolic compounds and chloroplast formation in tea callus tissues. Sov. Plant Physiol. 36: 65-71
  25. Folin, O. and W. Denis (1912) On phosphotungastic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12:239-243
  26. Bae, J. H. and J. E. Kim (2004) Inhibitory effect of Plantago asiatica extracts on the growth of gastric and colon cancer cell lines. Food Sci. Biotechnol. 13: 11-16
  27. Choi, D. B., W. S. Cha, S. H. Kang, and B. R. Lee (2004) Effect of Pleurotus ferulae extracts on viability of human lung cancer and cervical cancer cell lines. Biotechnol. Bioprocess Eng. 9: 356-361 https://doi.org/10.1007/BF02933057
  28. Shibasaki-Kitakawa, N., J. Takeishi, and T. Yonemoto (2003) Improvement of catechin productivity in suspension cultures of tea callus cells. Biotechnol. Prog. 19: 655-658 https://doi.org/10.1021/bp025539a