Mycobiology

The Korean Society of Mycology (한국균학회)
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Antitumor and Antioxidant Activities of the Extracts from Fruiting Body of Phellinus linteus

  • Published : 2006.12.31
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Abstract

Fruiting bodies of Phellinus linteus were extracted by hot water and alkali methods. Sugar contents of PL-H (hot water extract) and PL-A (alkali water extract) were 81.1%, 37.4% and protein contents were 6.2%, 21.8%, respectively. Amino acid pattern showed that two extracts contained large amount of aspartic acid and alanine. Two extracts showed characteristic IR absorption pattern for glycosidic bond at $890\;cm^{-1}$. PL-H was divided two fractions by gel filtration chromatography and the molecular weights of each fraction were estimated to be about 10 kD and 225 kD, respectively and also PL-A was estimated 10 kD. Two extracts showed strong antitumor, immunomodulating and antioxidant activities, and were compared with commercialized glycopeptide anticancer drugs.

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References

  1. Bao, X. F., Li, Y. Z., Ruan, L. and Fang, J. N. 2002. Purification, characterization, and modification of T lymphocyte stimulating polysaccharide from spore of Ganoderma lucidum. Chemical Pharmaceutical Bulletin 50: 623-629 https://doi.org/10.1248/cpb.50.623
  2. Chi, J. H., Ha, T. M., Kim, Y. H. and Ryo, Y. D. 1996. Studies on the main factors affecting the mycelial growth of Phellinus linteus. Korean Journal of Mycology 24: 214-222
  3. Cho, J. H., Cho, S. D., Hu, H., Kim, S. H., Lee, S. K., Lee, Y. S. and Kang, K. S. 2002. The role of ERK1/2 and p38 MAP kinases in the prevention mechanism of mushroom Phellinus linteus against the inhibition of gap junction intercellular communication by hydrogen peroxide. Carcinogen 23: 1164-1169
  4. Chung, K. S., Kim, H. S., Kim, K. Y., Han, M. W. and Kim, K. H. 1993. Effects of Kp, an antitumor protein polysaccharide from mycelial culture of Phellinus linteus on the humoral immune response of tumor bearing ICR mice to sheep red blood cells. Archives of Pharmacal Research 16: 336-338 https://doi.org/10.1007/BF02977527
  5. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28: 350-356 https://doi.org/10.1021/ac60111a017
  6. Fukushima, M. 1989. The over dose of drugs in Japan. Nature 342: 850-851 https://doi.org/10.1038/342850a0
  7. Han, S. B., Lee, C. W., Jeon, Y. J., Hong, N. D., Yoo, I. D. and Yang, K. H. 1999. The Inhibitory effect of polysaccharides isolated from Phellinus Iinteus on tumor growth and metastasis. Immunopharmacology 41: 157-164 https://doi.org/10.1016/S0162-3109(98)00063-0
  8. Hibbs, J. B., Taintor, R. R., Vavrin, Z. and Rachlin, E. M. 1988. Nitric oxide : a cytotoxic activated macrophage effector molecule. Biochemical Biophysical Research Communication 157: 87-94 https://doi.org/10.1016/S0006-291X(88)80015-9
  9. Hyun, J. W., Choi, E.C. and Kim, B.K. 1990. Studies on constituents of higher fungi of Korea (LXVII): antitumor component of the basidocarp of Ganoderma lucidum. Korean Journal of Mycology 18: 58-69
  10. Ikekawa, T., Nakanishi, M., Uehara, N. and Chihara, G 1968. Antitumor action of some basidomycetes, especially Phellinus linteus. Gann.59: 155-157
  11. Jong, S. C., Birmingham, J.M. and Pai, S.H. 1999. Immunomodulatory substances of fungal origin. EOS-J. Journal of Immunology Immunopharmacology 11: 115-122
  12. Kang, T. S., Lee, D. G and Lee, S. Y. 1997. Isolation and mycelial submerged cultivation of Phellinus sp. Korean Journal of Mycology 25: 257-267
  13. Kim, G. Y., Oh, Y. H. and Park, Y. M. 2003a. Acidic polysaccharide isolate from Phellinus linteus induces nitric oxide-mediated tumoricidal activity of macrophages through protein tyrosine kinase and protein kinase C. Biochemical Biophysical Research Communication 309: 399-407 https://doi.org/10.1016/j.bbrc.2003.08.018
  14. Kim, G. Y., Park, H. S., Nam, G. H., Lee, S. J. and Lee, J. D. 2003b. Purification and characterization from the fruiting body of Phellinus linteus (Berk. & M.A Curtis) Teng. Bioresource Technology 89: 81-87 https://doi.org/10.1016/S0960-8524(02)00273-0
  15. Kim, G Y., Park, S. K., Lee, M. K., Lee, S. H., Oh, Y. H., Kwak, J. Y., Yoon, S., Lee, J. D. and Park, Y. M. 2003c. Proteoglycan isolated from Phellinus linteus activates murine B lymphocytes via protein kinase C and protein tyrosine kinase. International Immunopharmacology 3: 1281-1292 https://doi.org/10.1016/S1567-5769(03)00115-2
  16. Kim, H. M., Han, S. B., Oh, G. T., Kim, Y. H., Hong, D. H., Hong, N. D. and Yoo, I. D. 1996. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. International Immunopharmacology 18: 295-303 https://doi.org/10.1016/0192-0561(96)00028-8
  17. Kim, S. H., Song, Y. S., Kim, S. K., Kim, B. C., Lim, C. J. and Park, E. H. 2004 Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus. Journal of Ethnopharmacology 93: 141-146 https://doi.org/10.1016/j.jep.2004.03.048
  18. Law, S. K. and Reid, K. B. M. 1988. Complement. IRL Press Oxford. UK. Pp 1-2
  19. Lee, J. H., Cho, S. M., Kim, M. H., Hong, N. D. and Yoo, I. D. 1997. Immunostimulating activity of polysaccharides from mycelia of Phellinus linteus grown under different culture conditions. Journal of Microbiology and Biotechnology 7: 52-55
  20. Ma, Y., Mizuno, T. and Chihara, G 1990. Antitumor polysaccharides isolated from a Chinese mushroom, Huangmo, the fruiting body of Hohenbuehelia serotina, Agricultural Biological Chemistry 55: 2701-2710
  21. Moncad, S., Palmer, R. M. J. and Higgs, E. A. 1991. Nitric oxide: Physiology, pathology, and pharmacology. Pharmaceutical Review 43: 109-141
  22. Okuda, T., Yoshioka, Y., Ikekawa, T., Chihara, G and Nishioka, K. 1972. Anticomplementary activity of anti-tumor polysacchardes. Nature New Biology 238: 59-60 https://doi.org/10.1038/238059a0
  23. Song, K. S., Cho, S. M., Lee, J. H., Kim, H. M., Han, S. B., Ko, K. S. and Yoo, I. D. 1995. B-lymphocyte-stimulating polysaccharide from mushroom. Phellinus linteus. Chemical Pharmaceutical Bulletin 43: 2105-2108 https://doi.org/10.1248/cpb.43.2105
  24. Song, Y. S., Kim, S. H., Sa, J. H., Jin, C. B., Lim, C. J. and Park, E. H. 2003. Anti-angiogenic, antioxidant and xanthine oxidase inhibition activities of the mushoroom Phellinus linteus. Journal of Ethnopharmacology 88: 113-116 https://doi.org/10.1016/S0378-8741(03)00178-8
  25. Stoscheck, C. M. 1990. Quantitation of Protein. Methods in Enzymology 182: 50-69 https://doi.org/10.1016/0076-6879(90)82008-P
  26. Suzuki, I., Hashimoto, K., Oikawa, S., Osawa, M. and Yadomoe, T. 1989. Antitumor and immunomodulating activities of a $\beta$-glucan obtained form liquid cultured Grifola fromdosa. Chemical Pharmaceutical Bulletin 37: 410-413 https://doi.org/10.1248/cpb.37.410
  27. Wasser, S. P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulationg polysaccharides. Applied Microbiology and Biotechnology 60: 258-274 https://doi.org/10.1007/s00253-002-1076-7
  28. Zhang, P., Zhang, L. and Cheng, S. 1999. Chemical structure and molecular weight of $\alpha$-(1$\rightarrow$3)-D glucan from Lentius edodes. Bioscience Biotechnology Biochemistry 63: 1197-1202 https://doi.org/10.1271/bbb.63.1197

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