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Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

  • Cho, Jung-Hee (Department of Agricultural Biology, Chungbuk National University) ;
  • Lee, Seung-Eun (Department of Agricultural Biology, Chungbuk National University) ;
  • Chang, Who-Bong (Chungbuk Agricultural Research and Extension Service) ;
  • Cha, Jae-Soon (Department of Agricultural Biology, Chungbuk National University)
  • Published : 2006.06.30

Abstract

Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.

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  2. Current Technologies and Related Issues for Mushroom Transformation vol.43, pp.1, 2015, https://doi.org/10.5941/MYCO.2015.43.1.1
  3. Establishment of an efficient transformation system for Pleurotus ostreatus vol.33, pp.12, 2017, https://doi.org/10.1007/s11274-017-2378-3
  4. Advances, Problems, and Prospects of Genetic Transformation of Fungi vol.52, pp.2, 2018, https://doi.org/10.3103/S009545271802007X