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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Characterization of ORF39 from Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus, the Gene Containing RNA Recognition Motif

  • Xu, Hai-Jun (Institute of Insect Sciences, Zhejiang University) ;
  • Liu, Yan-He (Institute of Insect Sciences, Zhejiang University) ;
  • Yang, Zhang-Nv (Institute of Insect Sciences, Zhejiang University) ;
  • Zhang, Chuan-Xi (Institute of Insect Sciences, Zhejiang University)
  • Received : 2005.10.19
  • Accepted : 2006.01.20
  • Published : 2006.05.31
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Abstract

In the genome of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus, open reading frame 39 (Ha39) is the only gene predicted to encode an RNA recognition protein. Computer analysis revealed that Ha39 homologues were found in 15 NPVs, but not in GVs. Its transcripts were detected from 3 through 72 hours post infection (h p.i.) using RT-PCR and Northern blot analysis. The protein was detected in infected-cell lysates from 6 h p.i. Western blot assay of ODV and BV preparations revealed that Ha39 encodes a structural protein associated with BVs. Additionally, immunofluorescence microscopy demonstrated that the protein was present within cytoplasm in virus-infected cells, but not in the nuclear region.

Keywords

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