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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Korean BAC Library Construction and Characterization of HLA-DRA, HLA-DRB3

  • Park, Mi-Hyun (Center for Genome Science, National Institute of Health) ;
  • Lee, Hye-Ja (Center for Genome Science, National Institute of Health) ;
  • Bok, Jeong (Center for Genome Science, National Institute of Health) ;
  • Kim, Cheol-Hwan (Dong-A SeeTech, Co. Ltd.) ;
  • Hong, Seong-Tshool (Department of Microbiology, Chonbuk National University Medical School) ;
  • Park, Chan (Center for Genome Science, National Institute of Health) ;
  • Kimm, Ku-Chan (Center for Genome Science, National Institute of Health) ;
  • Oh, Berm-Seok (Center for Genome Science, National Institute of Health) ;
  • Lee, Jong-Young (Center for Genome Science, National Institute of Health)
  • Received : 2006.01.10
  • Accepted : 2006.04.13
  • Published : 2006.07.31
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Abstract

A human bacterial artificial chromosome (BAC) library was constructed with high molecular weight DNA extracted from the blood of a male Korean. This Korean BAC library contains 100,224 clones of insert size ranging from 70 to 150 kb, with an average size of 86 kb, corresponding to a 2.9-fold redundancy of the genome. The average insert size was determined from 288 randomly selected BAC clones that were well distributed among all the chromosomes. We developed a pooling system and three-step PCR screen for the Korean BAC library to isolate desired BAC clones, and we confirmed its utility using primer pairs designed for one of the clones. The Korean BAC library and screening pools will allow PCR-based screening of the Korean genome for any gene of interest. We also determined the allele types of HLA-DRA and HLA-DRB3 of clone KB55453, located in the HLA class II region on chromosome 6p21.3. The HLA-DRA and DRB3 genes in this clone were identified as the DRA*010202 and DRB3*01010201 types, respectively. The haplotype found in this library will provide useful information in future human disease studies.

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References

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