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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Effective Chemopreventive Activity of Genistein against Human Breast Cancer Cells

  • Shon, Yun-Hee (Intractable Disease Research Center, Dongguk University) ;
  • Park, Sun-Dong (Department of Herbal Pharmacology, College of Oriental Medicine and Cardiovascular Medical Research Center, Dongguk University) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University)
  • Received : 2006.03.07
  • Accepted : 2006.05.02
  • Published : 2006.07.31
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Abstract

Chemopreventive and cytotoxic effect of genistein against human breast cancer cell lines was investigated. Genistein inhibited cell proliferation in estrogen receptor-positive (MCF-7) and estrogen receptor-negative (MDA-MB-231) human breast carcinoma cell lines. Cytochrome P450 (CYP) 1A1-mediated ethoxyresorufin O-deethylase (EROD) activity was inhibited by genistein in a concentrationdependent manner. Genistein significantly inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cyclooxy-genase-2 activity and protein expression at the concentrations of 10 (p < 0.05), 25 (p < 0.05) and 50 mM (p < 0.01). In addition, ornithine decarboxylase (ODC) activity was reduced to 53.8 % of the control after 6 h treatment with 50 mM genistein in MCF-7 breast cancer cells. These results suggest that genistein could be of therapeutic value in preventing human breast cancer.

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References

  1. Begg, L., Kuller, L. H., Gutai, J. P., Caggiula, A. G., Wolmask, N. and Watson, C. G. (1987) Endogenous sex hormone levels and breast cancer risk. Genetic Epidemiol. 4, 233-247 https://doi.org/10.1002/gepi.1370040402
  2. Canizares, F., Salinas, J., de las Heras, M., Diaz, J., Tovar, I., Martinez, P. and Penafiel, R. (1999) Prognostic value of ornithine decarboxylase and polyamines in human breast cancer: correlation with clinicopathologic parameters. Clin. Cancer Res. 5, 2035-2041
  3. Cavalieri, E. L., Stack, D. E., Devanesan, P. D., Todorovic, R., Dwivedy, I., Higginbotham, S., Johansson, S. L., Patil, K. D., Gross, M. L., Gooden, J. K., Ramanathan, R., Cerny, R. L. and Rogan, E. G. (1997) Molecular origin of cancer: catechol estrogen-3,4-quinones as endogenous tumor initiators. Proc. Natl. Acad. Sci. USA 94, 10937-10942 https://doi.org/10.1073/pnas.94.20.10937
  4. Fotsis, T., Pepper, M. S., Aktas, E., Breit, S., Rasku, S., Adlercreutz, H., Wahala, K., Montesano, R. and Schweigerer, L. (1997) Flavonoids, dietary-derived inhibitors of cell proliferation and in vitro angiogenesis. Cancer Res. 57, 2916-2921
  5. Huang, Z., Fasco, M. J., Figge, H. L., Keyomarsi, K. and Kaminsky, L. S. (1996) Expression of cytochromes P450 in human breast tissue and tumors. Drug Metab. Dispos. 24, 899-905
  6. Kim, S. U., Lee, K. M., Park, S. K., Yoo, K. Y., Noh, D. Y., Choe, K. J., Ahn, S. H., Hirvonen, A. and Kang, D. H. (2004) Genetic polymorphism of glutathione S-transferase P1 and breast cancer risk. J. Biochem. Mol. Biol. 37, 582-585 https://doi.org/10.5483/BMBRep.2004.37.5.582
  7. Kudo, I. and Murakami, M. (2005) Prostaglandin E synthase, a terminal enzyme for prostaglandin E$_{2}$ biosynthesis. J. Biochem. Mol. Biol. 38, 633-638 https://doi.org/10.5483/BMBRep.2005.38.6.633
  8. Liu, X. H., Wiley, H. S. and Meikle, A. W. (1993) Androgens regulate proliferation of human prostate cancer cells in culture by increasing transforming growth factor-$\alpha$ (TGF-$\alpha$) and epidermal growth factor (EGF)/TGF-2 receptor. J. Clin. Endocrinol. Metab. 77, 1472-1478 https://doi.org/10.1210/jc.77.6.1472
  9. Mazhar, D., Ang, R. and Waxman, J. (2006) COX inhibitors and breast cancer. Br. J. Cancer 94, 346-350 https://doi.org/10.1038/sj.bjc.6602942
  10. Mimori, K., Mori, M., Shiraishi, T., Tanaka, S., Haraguchi, M., Ueo, H., Shirasaka, C. and Akiyoshi, T. (1998) Expression of ornithine decarboxylase mRNA and c-myc mRNA in breast tumors. Int. J. Oncol. 12, 597-601
  11. Pike, M. C., Spicer, D. V., Dahmoush, L. and Press, M. F. (1993) Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk. Epidemiol. Rev. 15, 17-35 https://doi.org/10.1093/oxfordjournals.epirev.a036102
  12. Schrey, M. P. and Patel, K. V. (1995) Prostaglandin E$_{2}$ production and metabolism in human breast cancer cells and breast fibroblasts. Regulation by inflammatory mediators. Br. J. Cancer. 72, 1412-1419 https://doi.org/10.1038/bjc.1995.523
  13. Shon, Y. H. and Nam, K. S. (2003) Inhibition of polyamine biosynthesis of Acanthamoeba castellanii and 12-O-tetradecanoylphorbol-13-acetate-induced ornithine decarboxylase activity by chitosanoligosaccharide. Biotechnol. Lett. 25, 701-704 https://doi.org/10.1023/A:1023480701270
  14. Shon, Y. H., Nam, K. S. and Kim, M. K. (2004) Cancer chemopreventive potential of Scenedesmus spp. cultured in medium containing bioreacted swine urine. J. Microbiol. Biotechnol. 14, 158-161
  15. Spink, D. C., Spink, B. C., Cao, J. Q., DePasquale, J. A., Penteoost, B. T., Fasco, M. J., Li, Y. and Sutter, T. R. (1998) Differential expression of CYP1A1 and CYP1B1 in human breast epithelial cells and breast tumor cells. Carcinogenesis 19, 291-298 https://doi.org/10.1093/carcin/19.2.291
  16. Steele, V. E. (2003) Current mechanistic approaches to the chemoprevention of cancer. J. Biochem. Mol. Biol. 36, 78-81 https://doi.org/10.5483/BMBRep.2003.36.1.078
  17. Thomas, T. and Kiang, D. T. (1987) Structural alterations and stabilization of rabbit uterine estrogen receptors by natural polyamines. Cancer Res. 47, 1799-1804

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