Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Antimicrobial Effect of Roselle (Hibiscus sabdariffa L.) Petal Extracts on Food-Borne Microorganisms

  • Published : 2006.04.30
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Abstract

In this study, we investigated the antimicrobial effect of 14 different herbal petal extracts on various foodborne and food spoilage bacteria. Herbal petal extracts were prepared with 70% ethanol followed by sequential hexane, chloroform, ethyl acetate, n-butanol, and water fractionation. Antimicrobial activity was highest in the ethanol fraction from roselle (Hibiscus sabdariffa L.) petals as determined by the paper disc method. The roselle ethanol extract retarded the growth of food spoilage bacteria in kimbap (rice rolled in dried laver). Foodborne microorganisms (e.g. Bacillus cereus and Clostridium perfringens), on the other hand, were most efficiently inhibited by the ethyl acetate fraction of the roselle petal extract as determined by growth inhibition curves. Our study shows that roselle petals harbor antimicrobial activity against foodborne and food spoilage microorganisms. The critical ingredient is highly enriched in the ethyl acetate fraction of the extract.

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References

  1. Lue KH, Lee SS. The Illustrated Book of New Vegetable. Herbworld Press, Seoul (1998)
  2. Tyler VE. The Honest Herbal, 3rd ed. Haworth Press Inc., Binghampton, New York, USA. pp. 57-73 (1993)
  3. Tyler VE. Herbs of Choice: The Therapeutic Use of Phytomedicinals. Haworth Press, New York, NY, USA. pp. 126-145 (1994)
  4. Balchin ML. Essential oils and 'aromatherapy': their modem role in healing. J. Rus. Soc. Health 117: 324-329 (1997) https://doi.org/10.1177/146642409711700511
  5. Eisenberg DM, Kessler RC, Foster C, Norlock FE, Calkins DR, Delbanco TL. Unconventional medicine in the United States, preference, costs and patterns of use. New Engl. J. Med. 328: 246-252 (1993) https://doi.org/10.1056/NEJM199301283280406
  6. Lee KS, Kim MG, Lee KY. Antimicrobial effect of the extracts of cactus Chounnyouncho (Opuntia humifusa) against food borne pathogens. J. Korean Soc. Food Sci. Nutr. 33: 1268-1272 (2004) https://doi.org/10.3746/jkfn.2004.33.8.1268
  7. Davidson PM, Parish ME. Methods for testing the efficacy of food antimicrobials. Food Technol. 43: 148-155 (1989)
  8. Bae JH. Antimicrobial effect of Sophora angustifolia extracts on food-borne pathogens. Food Sci. BioTechnol. 14: 311-316 (2005)
  9. Montanari G, Borsari A, Chiavari C, Ferri G, Zambonelli C, Grazia L. Morphological and phenotypical characterization of Bacillus sporothermodurans. J. Appl. Microbiol. 97: 802-809 (2004) https://doi.org/10.1111/j.1365-2672.2004.02371.x
  10. Lee JY, Kim YS, Shin DH. Antimicrobial synergistic effects of linolenic acid in combination with preservatives against food-borne bacteria. Food Sci. Biotechnol. 13: 323-327 (2004)
  11. El-Shayeb NM, Mabrook SS. Utilization of some edible and medicinal plants to inhibit aflatoxin formation. Nutr. Rep. Int. 29: 273-282 (1984)
  12. Ali BH, Wabel NA, Blunden G. Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L.: A Review. Phytother. Res. 19: 369-375 (2005) https://doi.org/10.1002/ptr.1628
  13. Oangrade H, Mishra SH, Kaushal R. Antimicrobial activity of the oil and unsaponifiable matter of red roselle. Indian Drug. 16: 147-148 (1979)
  14. Esselen WB, Sammy GM. Roselle: a natural red colorant for foods? Food Prod. Dev. 7: 80-82 (1975)
  15. Seshadri TR, Thakur RS. Constitution of gossytrin, a new glycoside from the flower petals of Hibiscus sabdariffa. J. Indian Chern. Soc. 38: 649-651 (1961)