Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Synergistic Antimicrobial Action of Thymol and Sodium Bisulfate against Burkholderia cepacia and Xanthomonas maltophilia Isolated from the Space Shuttle Water System

  • Kim, Du-Woon (Department of Food and Nutrition, Chonnam National University) ;
  • Day, Donal F. (The Audubon Sugar Institute, LSU Agricultural Experiment Station)
  • Published : 2006.04.30
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Abstract

A combination of thymol and sodium bisulfate was found to be an effective biocidal agent against strains of Burkholderia cepacia and of Xanthomonas maltophilia that were found in the space shuttle water system. Potassium iodide (KI), the biocidal agent used in the past, had a minimum inhibitory concentration (MIC) of 50,000 ppm against the two B. cepacia (541 STS-81 and 1119 STS-91) strains, whereas that of thymol and sodium bisulfate was 2,400 and 950 ppm, which was 21 and 53 times lower than that of KI for B. cepacia, respectively. The MIC value for the combination of thymol and sodium bisulfate was 4 times lower than that for thymol or sodium bisulfate alone against B. cepacia (541 STS-81, 1119 STS-91) or Pseudomonas cepacia (ATCC 31941). The fractional inhibitory concentration (FIC) of the combination of thymol and sodium bisulfate for all organisms tested was less than 0.5, indicating a strong synergistic effect.

Keywords

References

  1. Burkholder WH. Sour skin, a bacterial rot of onion bulbs. Phytopathology 40: 115-117 (1950)
  2. Balandreau J, Viallard V, Cournoyer B, Coenye T, Laevens S, Vandamme P. Burkholderia cepacia genomovar III is a common plant-associated bacterium. Appl. Environ. Microbiol. 67: 982-985 (2001) https://doi.org/10.1128/AEM.67.2.982-985.2001
  3. Olapade OA, Gao X, Leff LG Abundance of three bacterial populations in selected streams. Microbial Ecol. 49: 461-467 (2005) https://doi.org/10.1007/s00248-004-0030-x
  4. Miller SCM, Lipuma JJ, Parke JL. Culture-based and non-growthdependent detection of the Burkholderia cepacia complex in soil environments. Appl. Environ. Microbiol. 68: 3750-3758 (2002) https://doi.org/10.1128/AEM.68.8.3750-3758.2002
  5. Henry D, Campbell M, Mcgimpsey C, Clarke A, Louden L, Burns JL, Roe MH, Vandamme P, Speert D. Comparison of isolation media for recovery of Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis. J. Clin. Microbiol. 37: 1004-1007 (1999)
  6. Messi P, Guerrieri E, Bondi M. Antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin. Sci. Total Environ. 346: 213-219 (2005) https://doi.org/10.1016/j.scitotenv.2004.12.005
  7. Miller CH. Microbes in dental water units. J. Can. Dental Assoc. 24: 47-52 (1996)
  8. Day DF, Ott CM, Mayo JA, Kim D. Multi-component, safe biocidal complex. US Patent 6,692,757 (2004)
  9. Koenig DW, Mishra SK, Pierson DL. Removal of Burkholderia cepacia biofilms with oxidants. Biofouling 9: 51-62 (1995) https://doi.org/10.1080/08927019509378291
  10. Arena MP, Porter MD, Fritz JS. Rapid, specific determination of iodine and iodide by combined solid-phase extraction/diffuse reflectance spectroscopy. Anal. Chem.74: 185-190 (2002) https://doi.org/10.1021/ac0109366
  11. Mayo JA, Oertling KM, Andrieu SC. Bacteria biofilm. Clin. Preventive Dentistry. 12: 13-20 (1990)
  12. Bae JH. Antimicrobial effect of Sophora angustifolia extracts on food-borne pathogens. Food Sci. Biotechnol. 14: 311-316 (2005)
  13. Rhee JH, Park KH, Yoon KR, Yim CB, Lee IH. Isolation of Bacillus subtilis producing the Cheongkukjang with reduced off-flavor by suppressing the growth of bacteria causing off -flavor. Food Sci. Biotechnol. 13: 801-805 (2004)
  14. Berenbaum MC. A method for testing for synergy with any number of agents. J. Infect. Dis. 137: 122-130 (1978) https://doi.org/10.1093/infdis/137.2.122
  15. Sweeney CR, McDonnell S, Russell GE, Terzich M. Effect of sodium bisulfate on ammonia concentration, fly population, and manure pH in a horse bam. Am. J. Vet. Res. 57: 1795-1798 (1996)
  16. Hawa SG, Morrison GJ, Fleet GH. Method to rapidly enumerate Salmonella on chicken carcasses. J. Food Prot. 47: 932-936 (1984) https://doi.org/10.4315/0362-028X-47.12.932
  17. Marais JT. Dental equipment and method of operating such equipment. US Patent 6,878,287 (2005)
  18. Pond GJ. Butler MS. Multi-valve dental handpiece supply reservoir. US Patent 6 302 692 (2001)
  19. Childs SJ. Safety of the fluoroquinolone antibiotics. Infect. Urol. 13: 3-10 (2000)