Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Phenolic Glycosides Isolated from Safflower (Carthamus tinctorius L.) Seeds Increase the Alkaline Phosphatase (ALP) Activity of Human Osteoblast-like Cells

  • Kim, Dong-Hyun (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Jin-Hee (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Ahn, Eun-Mi (Department of Herbal Food Science, Daegu Haany University) ;
  • Lee, Youn-Hyung (Department of Biotechnology, Major in Horticultural Biotechnology, Kyung Hee University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Kim, In-Ho (Material Processing Technology Division, Korea Food Research Institute)
  • Published : 2006.10.30
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Abstract

The chemical compositions of the seeds of the safflower (Carthamus tinctorius L.) plant were evaluated to determine possible compound having proliferative effects on human osteoblast cells. Three-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test and alkaline phosphatase (ALP) activity were used to assess the effects of the isolates on the human osteoblast-like line (Saos-2). Activity guided fractionation led to the isolation of ALP activating lignin and alkaloid glycosides through the extraction of the seeds, solvent partitioning and repeated silica gel and octadecyl silica (ODS) column chromatographic separations. The data from Nuclear Magnetic Resonance (NMR), Mass (MS), and Infrared (IR) analyses enabled the determination of the chemical structure and characterization of two compounds as a tracheloside and an N-(p-coumaroyl)-serotonin mono-${\beta}$-D-glucopyranoside. These two compounds showed respectively $149.2{\pm}4.2$ and $138.9{\pm}3.5%$ ALP activity compared to the control when evaluated at a concentration of $100\;{\mu}g/mL$.

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References

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