Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Soy Isoflavones Do Not Alter the Effects of Fructooligosaccharide on the Intestinal Ecosystem of Colon-Cancer Model Rats

  • Published : 2006.12.31
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Abstract

This study sought to investigate any additive or interactive effects that soy isoflavones may have on the ecosystem of the gut, which is influenced by fructooligosaccharide (FOS) in colon-cancer model rats. Male Sprague-Dawley rats treated with 1,2-dimethylhydrazine were given experimental diets containing 0, 3, 6, or 9% FOS with or without 0.1% soy isoflavone for 12 weeks. In addition to the effects of FOS dosage on the gut ecosystem, dietary supplementation with soy isoflavone reduced the number of colonic aberrant crypts (ACs). The fecal weight, fecal pH, and gut transit time significantly decreased in a dose-dependent manner in rats fed FOS and the fecal concentration of bifidobacteria was higher in rats fed FOS than in control rats. The fecal output of total short-chain fatty acids, acetate, and propionate was significantly increased by the presence of FOS and was negatively correlated with the number of ACs, whereas the fecal output of butyrate showed no significant correlation with FOS dosage. The addition of soy isoflavone to the diet did not result in any significant differences in gut ecosystem parameters. Therefore, we conclude that the suppressive effect of soy isoflavone on ACs was not associated with the intestinal ecosystem, which was significantly altered by the dosage of FOS.

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References

  1. Messina M, Bennink M. Soyfoods, isoflavones and risk of colonic cancer: a review of the in vitro and in vivo data. Bailliere Clin. Endoc. 12: 707-728 (1998) https://doi.org/10.1016/S0950-351X(98)80012-8
  2. Tham DM, Gardner CD, Haskell WL. Clinical review 97: potential health benefits of dietary phytoestrogens: a review of the clinical, epidemiological and mechanistic evidence. J. Clin. Endocr. Metab. 83: 2223-2235 (1998) https://doi.org/10.1210/jc.83.7.2223
  3. Turner NJ, Thomson BM, Shaw IC. Bioactive isoflavones in functional foods: the importance of gut microflora on bioavailability. Nutr. Rev. 61: 204-213 (2003) https://doi.org/10.1301/nr.2003.jun.204-213
  4. Atkinson C, Frankenfeld CL, Lampe Jw. Gut bacterial metabolism of the soy isoflavone daidzein: exploring the relevance to human health. Exp. Biol. Med. 230: 155-170 (2005) https://doi.org/10.1177/153537020523000302
  5. Lim CC, Ferguson LR, Tannock Gw. Dietary fibres as 'prebiotics': implications for colorectal cancer. Mol. Nutr. Food Res. 49: 609-619 (2005) https://doi.org/10.1002/mnfr.200500015
  6. Gazzaniga JM, Lupton JR. Dilution effect of dietary fiber sources in vivo study in the rat. Nutr. Res. 7: 1261-1268 (1987) https://doi.org/10.1016/S0271-5317(87)80086-6
  7. Tokunaga R. Novel physiological function offructooligosaccharides. Biofactors 21: 89-94 (2004) https://doi.org/10.1002/biof.552210117
  8. Pierre F, Perrin P, Champ M, Bomet F, Meflah K, Menanteau J. Short-chain fructo-oligosaccharides reduce the occurrence of colon tumors and develop gut-associated lymphoid tissue in Min mice. Cancer Res. 57: 225-228 (1997)
  9. De Boever P, Deplancke B, Verstraete W. Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by supplementing a soygerm powder. J. Nutr. 130: 2599-2606 (2000)
  10. Steer TE, Johnson IT, Gee JM, Gibson GR. Metabolism of the soybean isoflavone glycoside genistin in vitro by human gut bacteria and the effect of prebiotics. Brit. J. Nutr. 90: 635-642 (2003) https://doi.org/10.1079/BJN2003949
  11. Pool-Zobel BL. Inulin-type fructans and reduction in colon cancer risk: review of experimental and human data. Brit. J. Nutr. 93: S73-S90 (2005) https://doi.org/10.1079/BJN20041349
  12. Brady LJ, Gallaher DD, Busta FF. The role of probiotic cultures in the prevention of colon cancer. J. Nutr. 130: 410S-414S (2000) https://doi.org/10.1093/jn/130.2.410S
  13. Rafter J. Probiotics and colon cancer. Best Prac Res. Cl. Ga. 17: 849-859 (2003)
  14. Pool-Zobel BL, Neudecker C, Domizlaff I, Ji S, Schillinger U, Rumney C, Moretti M, Vilarini I, Scassellati-Sforzolini R, Rowland I. Lactobacillus- and befidobacterium-mediated antigenotoxicity in the colon of rats. Nutr. Cancer 26: 365-380 (1996) https://doi.org/10.1080/01635589609514492
  15. D'Argenio G, Cosenza V, Delle Cave M, Iovino P, Delle Valle N, Lombardi G, Mazzacca Ci Butyrate enemas in experimental colitis and protection against large bowel cancer in a rat model. Gastroenterology 110: 1727-1734 (1996) https://doi.org/10.1053/gast.1996.v110.pm8964397
  16. American Institute of Nutrition. Report of the ad hoc committee on standards for nutrition studies. J. Nutr. 123: 1939-1951 (1993) https://doi.org/10.1093/jn/123.11.1939
  17. Bird RP. Role of aberrant crypt foci in understanding the pathogenesis of colon cancer. Cancer Lett. 93: 55-71 (1995) https://doi.org/10.1016/0304-3835(95)03788-X
  18. Ji GE, Kim IH, Lee SK. Investigation of selective medium for isolation and enumeration of Bacteriodes sp. from the feces of the Korean people. Korean J. Food Sci. Technol. 26: 295-299 (1994)
  19. Choi YS, Jang JH. Effects of rice bran and wheat bran on the levels of lipids in plasma, liver and feces, and short-chain fatty acids in large bowel of rats. Food Sci. Biotechnol. 10: 166-171 (2001)
  20. Harris PJ, Ferguson LR. Dietary fibre: its composition and role in protection against colorectal cancer. Mutat. Res. 290: 97-110 (1993) https://doi.org/10.1016/0027-5107(93)90037-G
  21. Jackson PE, Cooper DP, O'Connor PJO, Povey AC. The relationship between 1,2-dimethylhydrazine dose and the induction of colon tumours: tumour development in female SWR mice does not require a K-ras mutational event. Carcinogenesis 20: 509-513 (1999) https://doi.org/10.1093/carcin/20.3.509
  22. Onoue M, Kado S, Sakaitani Y, Uchida K, Morotomi M. Specific species of intestinal bacteria influence the induction of aberrant crypt foci by 1,2 dimethylhydrazine in rats. Cancer Lett. 113: 179-186 (1997) https://doi.org/10.1016/S0304-3835(97)04698-3
  23. Gallaher D, Stallings W, Blessing L, Busta F, Brady L. Probiotics, cecal microflora, and aberrant crypts in the rat colon. J. Nutr. 126: 1362-1371 (1996)
  24. Fu H, Shi YQ, Mo SJ. Effect of short-chain fatty acids on the proliferation and differentiation of the human colonic adenocarcinoma cell line Caco-2. Chinese J. Dig. Dis. 5: 115-117 (2004) https://doi.org/10.1111/j.1443-9573.2004.00167.x
  25. Hague A, Manning AM, Hanlon KA, Huschtscha L, Hart D, Paraskeva C. Sodium butyrate induces apoptosis in human colonic tumour cell lines in a p53-independent pathway: implications for the possible role of dietary fibre in the prevention of large-bowel cancer. Int. J. Cancer 55: 498-505 (1993) https://doi.org/10.1002/ijc.2910550329
  26. Heerde BG, Houston MA, Augenlicht LH. Potentiation by specific short-chain fatty acids of differentiation and apoptosis in human colonic carcinoma cell lines. Cancer Res. 54: 3288-3293 (1994)
  27. Sung HY, Choi YS, Cho SH, Yun Jw. Fructooligosaccharides alter profiles of fecal short-chain fatty acids and bile acids in rats. Food Sci. Biotechnol. 15: 51-56 (2006)
  28. Zoran DL, Tumer ND, Taddeo SS, Chapkin RS. Wheat bran diet reduces tumor incidence in a rat model of colon cancer independent of effects on distal luminal butyrate concentrations. J. Nutr. 127: 2217-2225 (1997)
  29. Cademi G, Luceri C, De Filippo C, Salvadori M, Giannini A, Tessitore L, Dolara P. Slow-release pellets of sodium butyrate do not modify azoxymethane (AOM)-induced intestinal carcinogenesis in F344 rats. Carcinogenesis 22: 525-527 (2001) https://doi.org/10.1093/carcin/22.3.525
  30. Lupton JR. Microbial degradation products influence colon cancer risk: the butyrate controversy. J. Nutr. 134: 479-482 (2004)
  31. Hakkak R, Korourian S, Ronis MJ, Johnston JM, Badger TM. Soy protein isolate consumption protects against azoxymethane-induced colon tumors in male rats. Cancer Lett. 166: 27-32 (2001) https://doi.org/10.1016/S0304-3835(01)00441-4
  32. Guo JY, Li X, Browning JD, Rottinghaus GE, Lubahn DB, Constantinou A, Bennink M, MacDonald RS. Dietary soy isoflavones and estrone protect ovariectomized ERalphaKO and wild-type mice from carcinogen-induced colon cancer. J. Nutr. 134: 179-182 (2004)
  33. Rao CV, Wang CX, Simi B, Lubet R, Kelloff G, Steele V, Reddy BS. Enhancement of experimental colon cancer by genistein. Cancer Res. 57: 3717-3722 (1997)
  34. Allred CD, Allred KF, Ju YH, Goeppinger TS, Doerge DR, Helferich Wei Soy processing influences growth of estrogendependent breast cancer tumors. Carcinogenesis 25: 1649-1657 (2004) https://doi.org/10.1093/carcin/bgh178
  35. Allred CD, Twaddle NC, Allred KF, Goeppinger TS, Churchwell MI, Ju YH, Helferich WG, Doerge DR. Soy processing affects metabolism and disposition of dietary isoflavones in ovariectomized Balb/c mice. J. Agr. Food Chem. 53: 8542-8550 (2005) https://doi.org/10.1021/jf051246w