Enhancement of L-lysine Productivity by Strain Improvement and Optimization of Fermentation Conditions in Corynebacterium glutamicum

Corynebacterium glutamicum 균주 개량 및 발효 공정 최적화에 의한 L-lysine 생산성 증진

  • Seo, Jin-Mi (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Hyun, Hyung-Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • 서진미 (한국외국어대학교 생명공학과) ;
  • 현형환 (한국외국어대학교 생명공학과)
  • Published : 2006.04.28


In order to minimize the reduction of lysine productivity by accumulation of lysine and byproducts in the end of fed-batch fermentations, a salt-tolerant mutant C14-49-3-15-7-3-20, which could grow at high concentrations of NaCl was isolated through mutagenesis from the Corynebacterium glutamicum mother strain I. In the evaluation of L-lysine productivity by fed-batch fermentations using a 5 L jar fermenter, the salt-tolerant mutant strain C14-49-3-15-7-3-20 produced 130.6 g/L of L-lysine with a 48.6% of yield. The mother strain I produced L-lysine concentration only 104.9 g/L with a yield 41.8%, implying the improvement of L-lysine productivity by introduction of salt-tolerance character.


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