Role of Peripheral Glutamate Receptors to Mechanical Hyperalgesia following Nerve Injury or Antidromic Stimulation of L5 Spinal Nerve in Rats with the Previous L5 Dorsal Rhizotomy

제5효후근을 절단한 백서에서 제5요척수신경의 신경손상이나 전기자극에 의한 기계적 과민통 생성에 있어서 말초 글루타민산 수용기의 역할

  • Jang, Jun Ho (Departments of physiology, Yonsei University College of Medicine) ;
  • Nam, Taick Sang (Departments of physiology, Yonsei University College of Medicine) ;
  • Yoon, Duck Mi (Departments of Anesthesiology & Pain Medicine, Yonsei University College of Medicine) ;
  • Leem, Joong Woo (Departments of physiology, Yonsei University College of Medicine) ;
  • Paik, Gwang Se (Departments of physiology, Yonsei University College of Medicine)
  • 장준호 (연세대학교 의과대학 생리학교실) ;
  • 남택상 (연세대학교 의과대학 생리학교실) ;
  • 윤덕미 (연세대학교 의과대학 마취통증의학교실) ;
  • 임중우 (연세대학교 의과대학 생리학교실) ;
  • 백광세 (연세대학교 의과대학 생리학교실)
  • Received : 2006.01.19
  • Accepted : 2006.04.28
  • Published : 2006.06.30

Abstract

Background: Peripheral nerve injury leads to neuropathic pain, including mechanical hyperalgesia (MH). Nerve discharges produced by an injury to the primary afferents cause the release of glutamate from both central and peripheral terminals. While the role of centrally released glutamate in MH has been well studied, relatively little is known about its peripheral role. This study was carried out to determine if the peripherally conducting nerve impulses and peripheral glutamate receptors contribute to the generation of neuropathic pain. Methods: Rats that had previously received a left L5 dorsal rhizotomy were subjected to a spinal nerve lesion (SNL) or brief electrical stimulation (ES, 4 Hz pulses for 5 min) of the left L5 spinal nerve. The paw withdrawal threshold (PWT) to von Frey filaments was measured. The effects of an intraplantar (i.pl.) injection of a glutamate receptor (GluR) antagonist or agonist on the changes in the SNL- or ES-produced PWT was investigated. Results: SNL produced MH, as evidenced by decrease in the PWT, which lasted for more than 42 days. ES also produced MH lasting for 7 days. MK-801 (NMDAR antagonist), DL-AP3 (group-I mGluR antagonist), and APDC (group-II mGluR agonist) delayed the onset of MH when an i.pl. injection was given before SNL. The same application blocked the onset of ES-induced MH. NBQX (AMPA receptor antagonist) had no effect on either the SNL- or ES-induced onset of MH. When drugs were given after SNL or ES, MK-801 reversed the MH, whereas NBQX, DL-AP3, and APDC had no effect. Conclusions: Peripherally conducting impulses play an important role in the generation of neuropathic pain, which is mediated by the peripheral glutamate receptors.

Keywords

Acknowledgement

Supported by : 연세대학교

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