Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Production of Bacterial Cellulose Using Waste of Beer Fermentation Broth

맥주발효 폐액을 이용한 미생물 셀룰로오스 생산

  • Park, Joog Kon (Department of Chemical Engineering, Kyungpook National University) ;
  • Hyun, Seung Hoon (Department of Chemical Engineering, Kyungpook National University) ;
  • Ahn, Won Sool (Department of Chemical Engineering, Kyemyung University)
  • 박중곤 (경북대학교 화학공학과) ;
  • 현승훈 (경북대학교 화학공학과) ;
  • 안원술 (계명대학교 화학공학과)
  • Received : 2005.11.30
  • Accepted : 2006.02.02
  • Published : 2006.02.28
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Abstract

Bacterial cellulose (BC) was produced by Gluconacetobacter hansenii PJK (KCTC 10505 BP) strains using the waste of beer fermentation broth. It contained more C and N than a basal medium with a small amount of S and more than 4％ ethanol. The amount of BC produced in a shaking culture using the waste of beer fermentation broth was nearly the same as that of a basal medium. The production of BC decreased in a shear stress field in a jar fermenter although the conversion of cellulose producing ($Cel^+$) cells to non-cellulose producing ($Cel^-$) mutants was not severe. This study showed that the waste of beer fermentation broth is an inexpensive carbon, nitrogen source with ethanol and thus a worthy substitute for the conventional medium for BC production.

에탄올과 함께 탄소, 질소원을 함유한 맥주 폐 발효액을 저렴한 대체배지로 사용하고 Gluconacetobacter hansenii PJK(KCTC 10505 BP) 균주를 이용하여 미생물셀룰로오스를 생산하였다. 맥주 폐 발효액은 기본배지보다도 탄소원과 질소원이 풍부하였으며 미량의 황과 4％ 이상의 에탄올을 함유하였다. 진탕배양에서 맥주 폐 발효액을 사용하여 생산된 셀룰로오스량은 기본배지를 사용하여 생산된 셀룰로오스량과 필적하였다. 교반배양에서는 셀룰로오스 생산균주($Cel^+$ 균주)가 셀룰로오스를 생산하지 못하는 균주($Cel^-$ 돌연변이주)로 전환되는 률은 낮았지만 셀룰로오스 생산량은 감소하였다.

Keywords

References

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